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Preparation of macranthoin G in eucommia ulmoides, and application of macranthoin G in neuroprotective medicine

A technique for neuroprotection and honeysuckle, applied in the preparation of neuroprotective drugs, in the field of preparing honeysuckle honeysuckle G, can solve the problem that the control of monomer components of honeysuckle G and the antiplatelet aggregation activity have not been seen yet. And other issues

Inactive Publication Date: 2014-02-05
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, in the biological activity evaluation experiment, Lonicera cinereus G showed a very significant activating effect on NK cells in vitro, without any anti-platelet aggregation activity (Chen Min et al., Acta Pharmaceutica Sinica, 1994, 29 (8) , 617~620)
However, so far, there has been no research report on the activity of the G monomer component of Lonicera cinereus in preventing and treating any neurodegenerative diseases.

Method used

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  • Preparation of macranthoin G in eucommia ulmoides, and application of macranthoin G in neuroprotective medicine
  • Preparation of macranthoin G in eucommia ulmoides, and application of macranthoin G in neuroprotective medicine
  • Preparation of macranthoin G in eucommia ulmoides, and application of macranthoin G in neuroprotective medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The Preparation of Lonicera G

[0024] (1) Take the crushed root bark of Eucommia ulmoides as raw material, add 95% ethanol aqueous solution at a mass ratio of 1:5, extract at room temperature for 3 times, each time for 12 hours, filter, and concentrate the filtrate under reduced pressure to the original 3% of the volume to obtain the crude extract;

[0025] (2) Add water with 2 times the mass of the crude extract, stir, add 3 times the normal hexane of the mass of the crude extract to extract 3 times, separate, add the remaining water layer to extract 3 times with methylene chloride with 3 times the mass of the crude extract, Separation, adding ethyl acetate with 3 times the mass of the crude extract to the remaining water layer and extracting 3 times, separating the ethyl acetate layer and the final water layer, concentrating the ethyl acetate layer under reduced pressure to obtain the ethyl acetate extract phase;

[0026] (3) The ethyl acetate extract phase was prep...

Embodiment 2

[0028] The Preparation of Lonicera G

[0029] (1) Take the pulverized Eucommia ulmoides wood as raw material, add 80% ethanol aqueous solution at a mass ratio of 1:10, heat and extract 5 times, 6 hours each time, filter, and concentrate the filtrate to its original volume under reduced pressure 5% of the crude extract;

[0030] (2) Add water with 3 times the mass of the crude extract, stir, add 4 times the normal hexane of the mass of the crude extract to extract 4 times, separate, add the remaining water layer to extract 4 times with dichloromethane with 4 times the mass of the crude extract, Separation, adding the remaining water layer to ethyl acetate with 4 times the weight of the crude extract for extraction 4 times, separating the ethyl acetate layer and the final water layer, and concentrating the ethyl acetate layer under reduced pressure to obtain the ethyl acetate extract phase;

[0031] (3) The ethyl acetate extract phase was prepared by silica gel column chromatog...

Embodiment 3

[0033] Lonicerin G vs Hydrogen Peroxide H 2 o 2Protective effect of induced oxidative stress injury in rat adrenal pheochromocytoma cells (PC12 cells)

[0034] (1) Cultivation of PC12 cells

[0035] PC12 cells were cultured in RPMI1640 medium containing 5% FBS, 10% HS, 100 U / ml penicillin and 100 μg / ml streptomycin at 37°C with 5% CO 2 cultured in an incubator.

[0036] (2) Effects of Lonicera aureus G on the survival rate of normal PC12 cells

[0037] The survival rate of PC12 cells treated with different concentrations (0, 3.125, 6.25, 12.5, 25 and 50 μM) of Lonicerin G for 24 hours was determined by MTT method. The result is as figure 1 As shown, compared with the control group that was not treated with Lonicerin G, the relative survival rate of PC12 cells in each group was almost 100%, that is, Lonicerin G versus H 2 o 2 Uninduced normal PC12 cells had little toxic effect.

[0038] (3) Ash felted Lonicera G vs. H 2 o 2 Induced effects on PC12 cell viability

[0...

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Abstract

The invention belongs to the technical fields of tree secondary metabolism active ingredients and natural medicines, and particularly relates to a method for preparing macranthoin G by taking medicinal tree seed eucommia ulmoides as a raw material, and use of the macranthoin G in preparation of a neuroprotective medicine. The macranthoin G is prepared from eucommia ulmoides tree plant materials as raw materials in manners of drawing, extracting and separating. Themacranthoin G is simple in preparation technology, low in cost, short in cycle and high in yield. An experiment proves that themacranthoin G plays a significant protection role on a model cell injury, and indicates that the macranthoin G can be applied to preparation of the neuroprotective medicine, so as to prevent and treat various neurodegenerative diseases.

Description

technical field [0001] The invention belongs to the technical field of active components of secondary metabolism of trees and natural medicines, and specifically relates to a method for preparing Lonicera arvensis G from medicinal tree Eucommia ulmoides and the application of Lonicerin G in the preparation of neuroprotective drugs . Background technique [0002] Eucommia ulmoides Oliv. is a plant of Eucommia genus Eucommia (Eucommiaceae), a deciduous tree with a height of more than 20 meters. Medicinal Eucommia ulmoides is the dry bark of Eucommia ulmoides, which is brittle and easy to break. After breaking, there are fine, white, and elastic silver threads. , 2004, 38(5), 40-44), the Chinese Pharmacopoeia stipulates that the bark of Eucommia ulmoides is used as a traditional Chinese medicine Eucommia ulmoides. Eucommia ulmoides is a national second-class rare tree species. Due to its ancient origin, it has extremely important research value and medicinal value in science....

Claims

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Application Information

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IPC IPC(8): C07C69/757C07C67/48A61P25/00A61P25/28
CPCA61K31/216C07C67/48C07C2601/14C07C69/757
Inventor 司传领吴磊胡卫成黄晓凤杜振国
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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