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Calculus enzyme and preparation method thereof

A calculus enzyme and calculus technology, applied in biochemical equipment and methods, methods based on microorganisms, enzymes, etc., can solve the problems of long treatment time, intolerance of patients, and large side effects, and achieve simple preparation methods and no toxic side effects , The effect of low production cost

Inactive Publication Date: 2014-01-29
容瑜
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Clinical practice has proved that the effect of Western medicine alone in the treatment of calculus is not ideal, and long-term use of Western medicine has serious side effects and is difficult for patients to tolerate.
When stone disease is severe, surgery is needed to solve the problem, causing great pain to people
In recent years, people have studied the use of traditional Chinese medicine to treat stone disease, but there are defects. Because the specificity of traditional Chinese medicine is not strong and it is mainly cold medicine, its side effects are difficult to use for the prevention of stone disease at ordinary times. It is still necessary to crush the stone first and then use traditional Chinese medicine to remove the stone. The treatment time is long and the curative effect is not significant

Method used

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  • Calculus enzyme and preparation method thereof
  • Calculus enzyme and preparation method thereof
  • Calculus enzyme and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1, the calculus enzyme described in this embodiment is obtained by using the following parts by weight of the raw and auxiliary materials to carry out microbial fermentation culture, leaching, concentration and refining by solid-state fermentation method:

[0049]

[0050] Wherein said carbon source refers to the materials of D-glucose, flour, chaff, wort, persimmon, sugar beet and walnut residue.

[0051] Wherein the nitrogen source is dry yeast, fish meal, bean cake powder, chitin, ammonium phosphate, and ammonium nitrate.

[0052] Wherein the nutrient salt is a mixed salt solution containing magnesium, potassium, calcium, zinc and selenium.

[0053] Wherein said auxin is the material of corn steep liquor.

[0054] Wherein the enzyme-producing inducer is crushed human calculus.

[0055] The calculus enzyme is used as an enzyme for degrading calculus, specifically as an application of food, drink, health care product, medicine or injection in dissolving...

Embodiment 2

[0086] Embodiment 2, the calculus enzyme described in this embodiment is obtained by the following parts by weight of raw and auxiliary materials using solid-state fermentation method for microbial fermentation culture, leaching, concentration, and refining:

[0087]

[0088]

[0089] Wherein said carbon source refers to the materials of wheat bran, sucrose, tangerine, orange, apple and banana.

[0090] Wherein said nitrogen source is the material of beef extract, dry yeast, peanut cake powder, shrimp shell, ammonium sulfate.

[0091] Wherein the nutrient salt is a mixed salt solution containing phosphorus, sulfur, sodium, iron, calcium, zinc, cobalt and manganese ions.

[0092] Wherein said growth hormone is the material of vitamin.

[0093] Wherein, the enzyme-producing inducer is a material of artificial bovine gallstones and pulverized human body stones.

[0094] The calculus enzyme is used as an enzyme for degrading calculus, specifically as an application of food...

Embodiment 3

[0126] Embodiment 3, the calculus enzyme described in this embodiment is obtained by the following parts by weight of raw and auxiliary materials using solid-state fermentation method for microbial fermentation culture, extraction, concentration, and refinement:

[0127]

[0128] Wherein the carbon source refers to D-fructose, D-sorbitol, starch hydrolyzed sugar, starch, corn flour, straw, sunflower disc, malt, papaya, pineapple, fruit peel, fruit pomace, beet pomace, celery, pearl powder, The material of walnut powder.

[0129] Wherein the nitrogen source is yeast extract, fish meal, peptone, protein, snail, fish skin, crab shell, chitin, urea, amine, amide, purine base, pyrimidine base, ammonium phosphate, ammonium chloride, ammonium carbonate, liquid ammonia of materials.

[0130] Wherein the nutrient salt is a mixed salt solution containing phosphorus, sulfur, magnesium, potassium, sodium, iron, calcium, zinc, cobalt, manganese and selenium ions.

[0131] Wherein said...

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Abstract

The invention discloses a calculus enzyme which is an enzyme capable of defusing calculus. The calculus enzyme can be applied to a human body as food, beverage, health-care products, drugs or injection to prevent and defuse body stones. The calculus enzyme is convenient to use, mild in reaction, and free of toxic and side effects, and can prevent and treat renal calculus, urinary stones, gastric concretion, gall-stones and the like inside the human body. Clinical practices prove that the stones with different sizes inside the human body can be mildly defused and removed without crushing the stones. The preparation method of the calculus enzyme comprises the following steps: adopting the following raw materials in parts by weight: 20-30 parts of carbon source, 1-10 parts of nitrogen source and 40-94 parts of water, and 1-10 parts of nutritive salt and 1-5 parts of auxin serving auxiliaries; inducing microorganisms to ferment, culture, digest, separate and concentrate, so as to obtain a liquid crude product by adopting an artificial ox-gall calculi or body stones by a solid fermentation method; carrying out spray drying to obtain a solid crude product; filtering and refining the liquid crude product, so as to obtain a liquid refined product; separating, settling, centrifuging, and freeze-drying the liquid refined product, so as to obtain a lyophilized product, namely a solid refined product.

Description

technical field [0001] The invention relates to the technical field of enzymes or composite enzyme products, in particular to a calculus enzyme for dissolving human calculi and a preparation method thereof. Background technique [0002] Calculus enzyme is an enzyme that can degrade calculus, and it can be well used to dissolve human calculus; it is an enzyme or compound obtained from relevant raw and auxiliary materials using solid-state fermentation method for microbial fermentation culture, leaching, concentration and refining type enzyme products. [0003] Reform and opening up have made our people gradually move from poverty to prosperity. After entering a well-off society, people eat more and eat better, but more people suffer from gallstones. The reason is that the substances eaten too much are not absorbed and transformed into beneficial substances, but transformed or accumulated into harmful substances. For example, sub-healthy people who eat high-fat foods, high-c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/00C12R1/80C12R1/685C12R1/885C12R1/69
CPCC12N9/00A61K38/00A61P1/14A61P13/12
Inventor 黄虹寓
Owner 容瑜
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