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Novel green process for extracting ganoderma lucidum polysaccharides

A Ganoderma lucidum polysaccharide and working pressure technology, applied in the field of microorganisms, can solve the problems of production cost and product quality, alcohol and labor consumption, low monosaccharide removal rate, etc., to reduce steam consumption, reduce membrane cost, and monosaccharide removal high rate effect

Inactive Publication Date: 2014-01-15
JIANGSU SHENHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, during the alcohol precipitation process, since monosaccharides are often accompanied by polysaccharides, the removal rate of monosaccharides is low, so multiple alcohol precipitations are required, which consumes more alcohol and labor, and the production cost is higher; at the same time, the traditional thermal Concentration method consumes a lot of steam, which has a great impact on production cost and product quality

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  • Novel green process for extracting ganoderma lucidum polysaccharides

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Ganoderma lucidum polysaccharide extraction method of the present invention is carried out according to the following steps:

[0026] 1. Source of Ganoderma lucidum fermentation liquid: Ganoderma lucidium, provided and identified by the China Agricultural Microorganism Culture Collection Management Center, preservation number ACCC50059. Transfer the strain to the slant medium for conventional culture at 26-28°C for 5-6 days to obtain the activated Ganoderma lucidum strain; inoculate the activated Ganoderma lucidum strain into the seed medium, wherein the composition of the seed medium is soybean cake powder 2.0%, sucrose 4.0%, yeast powder 2.0%, potassium dihydrogen phosphate 0.4%, magnesium sulfate 0.09%, defoamer 0.1%, prepared with water, pH value is natural, shake culture at 26-28°C for 2-3 days to obtain the first-class seed liquid of Ganoderma lucidum; Put the primary seed liquid into the secondary seed medium, wherein the secondary seed medium is composed of soyb...

Embodiment 2

[0032] Ganoderma lucidum polysaccharide extraction method of the present invention, carry out according to the following steps:

[0033] 1, microfiltration filtration: the Ganoderma lucidum fermented liquid 7500Kg (fermentation liquid mycelium content is 3.7%) in the embodiment 1 is filtered by microfiltration, obtains the wet mycelium of the microfiltration dialysate 6372Kg of Ganoderma lucidum and 1128Kg (water content 76.1%), the light transmittance of the dialysate is 84%, and the wet mycelia can be directly dried and pulverized at low temperature to obtain fermented Ganoderma lucidum powder. The membrane material used for microfiltration is a ceramic membrane with a pore size of 100nm. The specific process conditions are: the operating temperature is 50-60°C, the inlet pressure is 2.0bar, the outlet pressure is 1.0bar, and the pressure difference is 1.0bar.

[0034]2. Ultrafiltration filtration: 6372Kg of the microfiltration dialysate obtained in step 1 is filtered by ult...

Embodiment 3

[0038] Ganoderma lucidum polysaccharide extraction method of the present invention, carry out according to the following steps:

[0039] 1. Microfiltration filtration: 7500Kg of fermented liquid of Ganoderma lucidum (the mycelium content of the fermentation liquid is 3.7%) is filtered by microfiltration to obtain 6374Kg of microfiltration dialysate of Ganoderma lucidum and 1126Kg of wet mycelium (water content of 77%. %), the light transmittance of the dialysate is 80%, and the wet mycelium can be directly dried and pulverized at low temperature to obtain fermented Ganoderma lucidum powder. The membrane material used for microfiltration is a ceramic membrane with a pore size of 500nm. The specific process conditions are: the operating temperature is 50-60°C, the inlet pressure is 2.3bar, the outlet pressure is 0.9bar, and the pressure difference is 1.4bar.

[0040] 2, ultrafiltration filtration: the microfiltration dialysate 6374Kg obtained in step 1 is filtered by ultrafiltra...

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Abstract

The invention discloses a novel green process for extracting ganoderma lucidum polysaccharides and in particular relates to a method for extracting ganoderma lucidum polysaccharides by a double membrane process. According to the method, ganoderma lucidum polysaccharides are obtained by separating a lucid ganoderma fermentation liquor using a micro-filtration membrane and an ultrafiltration membrane under special conditions. According to the invention, the conventional ganoderma lucidum polysaccharide extraction mode of water extraction and ethanol precipitation is changed, the purification effect of ganoderma lucidum polysaccharides is improved, and the consumption of ethanol during production is reduced, therefore the economic benefit is increased. Besides, with adoption of the method, the emission of filtrate wastewater is reduced and the social benefit is remarkably increased.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a method for extracting fungal exopolysaccharides, more precisely, a new method for extracting ganoderma lucidum polysaccharides based on membrane technology. Background technique [0002] Ganoderma lucidum polysaccharide is a polysaccharide composed of glucose, arabinose, xylose, fucose, rhamnose, galactose and other monosaccharides, which is extracted and refined from the fermentation filtrate of Ganoderma lucidum by modern bioengineering technology Proteoglycan is one of the most effective components of Ganoderma lucidum. Experiments have proved that Ganoderma lucidum polysaccharide has many pharmacological activities: it can improve the body's immunity, accelerate blood microcirculation, improve blood oxygen supply capacity, reduce the ineffective oxygen consumption in the body's resting state, eliminate free radicals in the body, and improve the sealing of the b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 徐玲王文风张瑞华
Owner JIANGSU SHENHUA PHARMA
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