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Fingerprint spectrum detection method of Zhenju anti-hypertension tablets and application thereof

A technology of Zhenju Jiangya tablets and fingerprints, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problem of insufficient quality control methods for related components of wild chrysanthemum, and achieves good system applicability, good repeatability, and reduced The effect of operating errors

Active Publication Date: 2014-01-08
SHANGHAI LEIYUNSHANG PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In summary, the current research on the quality control methods of the related components of chrysanthemum in Zhenju Jiangya Tablets is not deep enough

Method used

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  • Fingerprint spectrum detection method of Zhenju anti-hypertension tablets and application thereof
  • Fingerprint spectrum detection method of Zhenju anti-hypertension tablets and application thereof
  • Fingerprint spectrum detection method of Zhenju anti-hypertension tablets and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Example 1 Determination of Chlorogenic Acid, Luteolin, Menghuaside, Hydrochlorothiazide and Rutin in Zhenju Jiangya Tablets

[0074] 1. Determination method:

[0075] 1.1 Chromatographic conditions

[0076] Instrument: Agilent1200 high performance liquid chromatography

[0077] Chromatographic column: Kromasil C18 (5μm, 4.6×250mm)

[0078] Mobile phase: Phase A is acetonitrile, phase B is 0.05% phosphoric acid aqueous solution, linear gradient elution (see Table 1)

[0079] Flow rate: 1.0mL / min Column temperature: 30℃ Detection wavelength: 334nm

[0080] Table 1 Gradient elution method

[0081]

[0082] 1.2 Preparation of solution

[0083] Preparation of reference solution (method 1): take chlorogenic acid, luteolin, and mongoside as reference substances and put them in a phosphorus pentoxide dryer for 24 hours in vacuum; take about 10 mg of luteolin reference substances and weigh them accurately. Put it in a 100mL measuring bottle, add methanol to dissolve, se...

Embodiment 2

[0144] Example 2 Establishment of Fingerprint of Zhenju Jiangya Tablets

[0145] 1. Detection method:

[0146] 1.1 Chromatographic conditions

[0147] Instrument: Agilent1200 high performance liquid chromatography

[0148] Chromatographic column: Kromasil C18 (5μm, 4.6×250mm)

[0149] Mobile phase: Phase A is acetonitrile, phase B is 0.05% phosphoric acid aqueous solution, linear gradient elution (see Table 1)

[0150] Flow rate: 1.0mL / min Column temperature: 30℃ Detection wavelength: 334nm

[0151] 1.2 Preparation of solution

[0152] Preparation of reference substance solution: take chlorogenic acid, luteolin, and mongoside reference substances and put them in a phosphorus pentoxide dryer for 24 hours in a vacuum; In the bottle, add methanol to dissolve, dilute to the mark, shake well, and use as luteolin stock solution. Take 10 mg each of chlorogenic acid reference substance and mongoside reference substance, weigh them accurately, put them in a 250mL measuring bottle...

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Abstract

The invention provides a fingerprint spectrum detection method of Zhenju anti-hypertension tablets and application of the method. The fingerprint spectrum detection method of the Zhenju anti-hypertension tablets comprises the steps of preparation of a test solution, namely evenly mixing a ground test sample and a methyl alcohol aqueous solution extract solvent with the volume percent of more than 30%, and then carrying out ultrasonic treatment for more than 30min to obtain the test solution after solid-liquid separation; measurement of the test solution, namely under appropriate conditions, measuring or obtaining the fingerprint spectrum of the test solution by high performance liquid chromatography. The invention further provides the application of the fingerprint spectrum detection method to the quality detection of the Zhenju anti-hypertension tablets or the content measurement of one or more of chlorogenic acid, galuteolin, linarin, hydrochlorothiazide, rutin and the like in the Zhenju anti-hypertension tablets. The method not only is suitable for measuring the finished product of the Zhenju antihypertension tablets, but also can be used for guiding the production process control or the technology improvement.

Description

technical field [0001] The invention relates to the field of quality detection of traditional Chinese medicinal materials, in particular to a fingerprint detection method and application of Zhenju Jiangya Tablets. Background technique [0002] The current standard of Zhenju Jiangya Tablets is recorded in the "Traditional Chinese Medicine Prescription Volume 20" (WS3-B3925-98), limited by the technical level at the time of development, the standard only stipulates the properties and identification, and the routine is carried out according to the general principles of preparations Project inspection, without formulating quantitative measurement items for main active ingredients, it is difficult to achieve quality control, and it is impossible to guarantee the effectiveness, safety and quality controllability of clinical use. In recent years, with the continuous deepening of the secondary development of products and the research and development of generic drugs, researchers hav...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 敬应春郭美兰蔡国琴张聪
Owner SHANGHAI LEIYUNSHANG PHARMA
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