Culture medium using waste pomegranate twigs as matrix and preparation method thereof
A preparation method and culture medium technology, applied in the field of agricultural microorganisms, can solve the shortage of raw materials for edible fungus cultivation, and achieve the effects of high sustainability and economic benefits, increasing varieties, and alleviating the shortage of raw materials
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Embodiment 1
[0035] The raw materials are: 38% pomegranate branches, 20% cottonseed hulls, 5% corncobs, 5% miscellaneous sawdust, 10% wine residue, 15% wheat bran, 4% oily dryness, 0.5% sucrose, 0.5% potassium dihydrogen phosphate, gypsum 1% and traditional Chinese medicine yeast 1%.
[0036] a. Crush pomegranate branches into particles with a particle size of 0.1 to 10 mm, and soak them in lime water with a mass concentration of 1% for 24 hours;
[0037] b. Mix the soaked pomegranate branches with cottonseed husks, corncobs, miscellaneous wood chips, wine residues, and traditional Chinese medicine yeasts, and adjust the water to about 60%. Sterilize under high pressure at 0.1 MPa for 2 hours at ℃, and cool down to room temperature to obtain a culture medium.
[0038] Inoculation, germination and fruiting are consistent with common edible fungus cultivation methods.
Embodiment 2
[0040] The raw materials are 50% of pomegranate branches, 10% of cottonseed hulls, 10% of corncobs, 6% of wine lees, 15% of wheat bran, 5% of oilseed, 1% of sucrose, 1% of potassium dihydrogen phosphate, 3% of gypsum, and traditional Chinese medicine yeast 1%.
[0041] a. Crush the pomegranate branches into particles with a particle size of 0.1 to 10 mm, and soak them in lime water with a mass concentration of 3% for 48 hours;
[0042] b. Mix the soaked pomegranate branches with cottonseed husks, corn cobs, wine residues, and Chinese medicine yeasts evenly and adjust the water to about 65%. After composting and fermenting for 8 days, add other raw materials and mix evenly. MPa sterilized for 1.5h, lowered to room temperature, to obtain the culture medium.
[0043] Inoculation, germination and fruiting are consistent with common edible fungus cultivation methods.
Embodiment 3
[0045] The raw materials are: 58% pomegranate branch, 10% cottonseed husk, 10% wine residue, 15% wheat bran, 4% oil keratin, 0.5% sucrose, 0.5% potassium dihydrogen phosphate, 1% gypsum, and 1% traditional Chinese medicine yeast.
[0046] a. Crush the pomegranate branches into particles with a particle size of 0.1 to 10 mm, and soak them in lime water with a mass concentration of 1% for 72 hours;
[0047] b. Mix the soaked pomegranate branches with wine residues, cottonseed husks, and traditional Chinese medicine yeast evenly and adjust the water to about 60%. After composting and fermenting for 5 days, add other raw materials and mix evenly, pack them into bags, and sterilize at 95°C for 12 hours , down to room temperature, to obtain the culture medium.
[0048] Inoculation, germination and fruiting are consistent with common edible fungus cultivation methods.
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