Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method and kit for establishing experimental model of diabetic foot ulcer big mouse infected by staphylococcus aureus

A technique for diabetic foot ulcer and staphylococcal infection, applied in the field of establishing experimental model of diabetic foot ulcer rats infected by Staphylococcus aureus

Inactive Publication Date: 2014-01-01
SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
View PDF1 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The results of literature research show that the existing animal models of diabetic foot ulcers mostly simulate a single pathogenic factor (such as peripheral arterial disease, neuropathy or infection), and there is a big gap between the similarity between the animal model and the actual simulation and the research purpose, and the actual The combination of two or more factors is more common in clinical practice

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method and kit for establishing experimental model of diabetic foot ulcer big mouse infected by staphylococcus aureus
  • Method and kit for establishing experimental model of diabetic foot ulcer big mouse infected by staphylococcus aureus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Rats with type 2 diabetes mellitus induced by high-fat feed combined with streptozotocin, using a scald apparatus to make deep second-degree burns to induce skin ulcers, and using subcutaneous injection of quality control bacteria Staphylococcus aureus-ATCC25923 (Staphylococcus aureus) strain to make A rat model of diabetic foot ulcer infection.

[0024] 1 Materials and methods

[0025] 1.1 Animals: SD male rats, SPF grade, body weight (180±10) g, provided by the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine.

[0026] 1.2 Main reagents and drugs: Streptozotocin (STZ), purchased from Alexis Company; high-fat feed (ingredients: basic feed 73%, lard 20%, milk powder 2%, cholesterol 1%, sucrose 4%; The basic feed formula: 15% barley, 31% wheat, 15% corn, 15% bran, 6% fishmeal, 18% soybean meal, oil and salt, the basic feed was purchased from the Naval Medical Research Institute of the Chinese People’s Liberation Army), high The preparat...

Embodiment 2

[0039] Example 2 Western medicine-recombinant bovine basic fibroblast growth factor for external use (trade name: Beifuji) intervention test results

[0040] 1. Methods: Diabetic control group (DM) without treatment; normal ulcer group (N), diabetic ulcer group (DU), S. aureus control group (SD-C), normal saline dressing change every day; S. aureus treatment group ( SD-T), Beifuji 800iu wound dressing was changed daily. Measure 0d, 3d, 10d after dressing change, wound area after healing, 3% pentobarbital sodium in 2ml Kg after healing -1 The dose was anesthetized by intraperitoneal injection, blood was collected from the abdominal aorta, serum was separated by centrifugation, and skin tissue was taken for examination.

[0041] 2. Data processing: SPSS16.0 was used for statistical analysis, and measurement data was used Indicates that the data conforming to normality and homogeneity of variance were analyzed by one-way analysis of variance, and the components were tested b...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for establishing an experimental model of a diabetic foot ulcer big mouse infected by staphylococcus aureus. The method includes the following steps that after the big mouse is fed on high-fat diet for eight weeks, streptozotocin is injected, and a diabetic model is manufactured. After the diabetic model is stabilized, II-degree scalding is performed on the skin of the big mouse, and after three days, subcutaneous injection of suspension liquid with staphylococcus aureus as the bacterial strain is performed in each ulcer position. In addition, the invention further discloses a kit for establishing the experimental model of the diabetic foot ulcer big mouse infected by the staphylococcus aureus, wherein the kit comprises the staphylococcus aureus suspension liquid. The diabetic foot ulcer experimental animal model detects variation of biochemical indexes after pharmacological intervention, and a result shows that the model has the infection features of diabetic foot ulcer and concurrent gram-positive bacteria. The model has the advantages of being short in ulcer wound surface healing time, sensitive to drug reaction and the like.

Description

technical field [0001] The invention belongs to the field of experimental animal models, and in particular relates to a method and a kit for establishing an experimental model of diabetic foot ulcer rats infected by Staphylococcus aureus. Background technique [0002] With the sharp increase in the incidence of diabetes in my country, the number of patients with diabetic ulcers has also increased. Establishing a disease model that truly simulates diabetic ulcers at the overall animal level is an important tool for medical research such as exploring the pathogenesis of diabetic ulcers and preclinical pharmacodynamic evaluation. It has very important scientific and clinical significance. [0003] At present, animal models of diabetic foot ulcers are mostly induced animal models. The results of literature research show that the existing animal models of diabetic foot ulcers mostly simulate a single pathogenic factor (such as peripheral arterial disease, neuropathy or infection)...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K35/74A61K31/7008A01K67/02
Inventor 柳国斌韩强
Owner SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com