Method for extracting, separating and preparing lignin monomers from schisandra chinensis

Abstract

The invention discloses a method for extracting, separating and preparing lignin monomers from schisandra chinensis. The method comprises the following steps: performing supercritical extraction on schisandra chinensis medicinal powder by using CO2 to obtain a crude extract; performing primary high-speed counter-current chromatographic separation on the crude extract; forming a solvent system A by using hexane, ethyl acetate, methyl alcohol and water in the volume ratio of (2-10):(0-10):(0-10):5, wherein the upper phase A is a stationary phase A and the lower phase A is a mobile phase A; preparing a first schizandrol, a second schizandrol and a third schizandrol; recovering the separated stationary A to serve as a sample to be tested; performing secondary high-speed counter-current chromatographic separation; forming a solvent system B by using hexane, ethyl acetate, methyl alcohol and water in the volume ratio of (1-10):(0-4):(0-9):1, wherein the upper phase B is a stationary phase B, and the lower phase B is a mobile phase B; preparing deoxyschizandrin and schisandrin b. By adopting the method which integrates supercritical extraction and high-speed counter-current chromatographic separation, the deoxyschizandrin and the schisandrin b can be directly used for separating and purifying subsequent monomers without complex sample treatment after the extraction; the steps of extracting and separating are simple and efficient.

Description

Technical field [0001] The invention belongs to the field of natural medicine chemistry, and relates to a fast and efficient combined preparation method for extracting, separating and purifying lignan monomer components from Schisandra. It specifically relates to the use of supercritical extraction combined with high-speed countercurrent chromatography to extract and separate the monomer components of Schisandra. Background technique [0002] Schisandra is the mature fruit of Schisandra chinensis (Turcz.) Baill or Schisandra sphenanthera Rehd.et Wils. The former is called "Northern Schisandra" and the latter is called "Southern Schisandra". It was first published in "Shen Nong's Materia Medica" and was listed as top grade. Schisandra is warm in nature, sweet and sour in taste, and belongs to the lung, heart and kidney meridians. It has the effects of astringent, astringent, nourishing qi, nourishing body fluid, nourishing the kidney and calming the heart. It can be used to tr...

AI Technical Summary

Problems solved by technology

But so far there is no report on the separation and purification of lignan monomers in Schisandra chinensis by supercritical extraction and high-speed countercurrent chromatography

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