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Cloud point extraction based psoralen and isopsoralen extracting method

A technology of isopsoralen and psoralen, which is applied in the field of extraction of plant active ingredients, can solve the problems of poor enrichment rate, low extraction rate, cumbersome operation, etc., and achieves simple operation, high extraction rate and low dosage little effect

Inactive Publication Date: 2013-11-27
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the main disadvantages are: cumbersome operation, low extraction rate and poor enrichment rate
However, organic solvents still need to be used, and the toxicity and pollution problems of these solvents cannot be avoided

Method used

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  • Cloud point extraction based psoralen and isopsoralen extracting method
  • Cloud point extraction based psoralen and isopsoralen extracting method
  • Cloud point extraction based psoralen and isopsoralen extracting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] The selection of embodiment 1 surfactant

[0064] Tried anionic surfactants: such as stearic acid, sodium dodecylbenzenesulfonate, etc.; cationic surfactants: such as quaternary ammonium compounds; zwitterionic surfactants: such as lecithin, amino acid type, betaine type, Lauryl alcohol polyoxyethylene ether (GENAPOL X080), etc.; nonionic surfactants: such as polyethylene glycol octylphenyl ether (Triton X series), fatty acid glycerides, fatty acid sorbitan, polysorbate, etc.;

[0065] The results show that the above-mentioned surfactants are all suitable for the method of extracting psoralen and isopsoralen based on cloud point extraction, and the extraction effect of GENAPOL X080 is the best, probably because GENAPOL X080 is a non-aromatic ring Fatty surfactant, no UV absorption at 210nm, will not interfere with the content determination of psoralen and isopsoralen.

[0066] In the following examples, GENAPOL X080 is used as the surfactant for cloud point extraction....

Embodiment 2

[0067] Example 2 Effect of surfactant concentration on extraction efficiency of psoralen and isopsoralen.

[0068] In the case of other extraction conditions unchanged, the effect of surfactant concentration on the extraction efficiency of psoralen and isopsoralen is as follows: figure 1 Shown, wherein, psoralen is the variation curve of the extraction efficiency of psoralen, and ispsoralen is the variation curve of different psoralen extraction efficiency;

[0069] It can be seen that when the concentration of surfactant increases from 1.0% to 15% (v / v, volume ratio), the extraction rate of psoralen and isopsoralen increases, while the concentration of surfactant increases from 15% to At 20%, the extraction rate increased but the change was not significant. Moreover, when it is shown that the concentration of the active agent increases to 20%, the extraction rate continues to increase, but the viscosity also increases, making it difficult to handle. Therefore, under this con...

Embodiment 4

[0072] The influence of embodiment 4 ultrasonic extraction time on extraction recovery rate

[0073] In the case of other extraction conditions unchanged, the ultrasonic-assisted extraction time of 5-60min was investigated. When the time is 30 minutes, the extraction rate of psoralen and isopsoralen is the highest. If the time is extended to more than 35 minutes, it may be because the active ingredients are easy to change under the long-term ultrasonic state. Therefore, in the steps of the following examples, 30 min is used as the ultrasonic dissolution time.

[0074] The addition of electrolyte and raising the temperature can facilitate the separation of the two phases, so the effects of equilibrium time, equilibrium temperature, and the amount of electrolyte on the extraction rate were investigated.

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Abstract

The invention discloses a cloud point extraction based psoralen and isopsoralen extracting method. The method comprises the following steps: fully mixing Psoralea corylifolia powder with a surfactant solution, and extracting under an ultrasonic condition; collecting the obtained extract liquid after extracting completion, centrifuging, collecting the obtained supernatant, adding an electrolyte for adjusting the ion intensity, balancing in a constant temperature water bath, and allowing the supernatant obtained after the centrifugation to stand in order to obtain an aqueous surfactant solution layer and a surfactant enrichment layer; and filtering the surfactant enrichment layer by an ultrafilter membrane after the two-phase layering, recovering the surfactant, and carrying out rotation evaporation of the obtained filtrate to obtain a fat-soluble crude extract containing psoralen and isopsoralen. The method has the advantages of no need of toxic harmful organic solvents, extracting rate increase, environmental pollution reduction, and suitableness for the industrialized large-scale extraction.

Description

technical field [0001] The invention belongs to the technical field of extraction of plant active ingredients, and relates to a method for extracting psoralen and isopsoralen based on cloud point extraction. Background technique [0002] Psoralen and isopsoralen are important active ingredients in psoralen. Due to the low content of psoralen and the like in plants, the extraction rate of monomers is lower. It is of great significance to study efficient extraction methods of psoralen and isopsoralen. In fact, the main reason for the low monomer extraction rate is that when the effective part (crude extract) is obtained from the plant in the first step, most of them use the traditional organic solvent extraction method. The process has a high reflux temperature, and the active ingredients are oxidized or decomposed. resulting in a low extraction rate. It can be seen that improving the extraction rate of psoraleae crude extract is the premise and key to realize high-yield ex...

Claims

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Application Information

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IPC IPC(8): C07D493/04
Inventor 陈惠罗晶王巧峰刘雪英姜茹
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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