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Detecting method for divalent mercury ions

A DNA molecule, single-stranded technology, applied in the field of divalent mercury ion detection, can solve the problems of insufficient specificity, insufficient sensitivity, complicated design and operation, etc., and achieve good accuracy, precision, and high selectivity.

Active Publication Date: 2013-11-13
BEIJING INST OF COLLABORATIVE INNOVATION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods are either not sensitive enough, not specific enough, or the design and operation are cumbersome and unsuitable for rapid on-site detection of mercury ions

Method used

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  • Detecting method for divalent mercury ions
  • Detecting method for divalent mercury ions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, immobilization of DNA capture probe

[0039] DNA Capture Probe 1: 5'-NH 2 -(CH 2 ) 6 -TACAAACA-3' (SEQ ID NO: 2);

[0040] DNA capture probe 2: 5'-NH 2 -(CH 2 ) 6 -TACAAACAAA-3' (Sequence Listing Sequence 3);

[0041] The DNA capture probes 1 and 2 are respectively modified on the evanescent wave optical fiber sensing probe according to the method comprising the following steps:

[0042] 1. Probe cleaning

[0043] Immerse the evanescent wave fiber optic sensing probe in piraha solution (concentrated H 2 SO 4 with H 2 o 2 The volume ratio of water is 3:1) for 30 minutes; then put it into an ultrasonic cleaner and wash it thoroughly with ultrapure water until the pH value of the cleaning solution is neutral, and finally dry it with nitrogen at room temperature and store in Store in a vacuum oven.

[0044] 2. Silanize the probe surface treated in step 1 according to the method comprising the following steps:

[0045] Put the probe treated in ste...

Embodiment 2

[0048] Example 2, Hg 2+ detection

[0049] 1. Hg 2+ Establishment of the Functional Relation between Concentration and Fluorescent Signal Intensity

[0050] 1. Load the evanescent wave optical fiber sensing probe whose surface is modified with the single-stranded DNA molecule A shown in Sequence 2 of the Sequence Listing obtained in Example 1 into the evanescent wave optical fiber sensing analysis platform;

[0051] 2. The known Hg 2+ The standard solution (solvent is water) with concentrations of 0, 10, 30, 100, 300 and 1000nmol / L were respectively added with the final concentration of 20nmol / L the 5' end modified by fluorescein Strand DNA molecule B is then pumped into the sample pool at 300 μL / min (the pool is equipped with an evanescent wave fiber optic sensing probe whose surface is modified with single-stranded DNA molecule A shown in sequence number 2 of the sequence listing) for 30 seconds, and further reaction 4min; the reaction principle is as follows figure 1 s...

Embodiment 3

[0060] Example 3, Hg 2+ detection

[0061] 1. Hg 2+ Establishment of the Functional Relation between Concentration and Fluorescent Signal Intensity

[0062] 1. Load the evanescent wave optical fiber sensing probe whose surface is modified with the single-stranded DNA molecule A shown in Sequence 3 of the Sequence Listing obtained in Example 1 into the evanescent wave optical fiber sensing analysis platform;

[0063] 2. The known Hg 2+ The standard solution (solvent is water) with concentrations of 0, 10, 30, 100, 300 and 1000nmol / L were respectively added with the final concentration of 20nmol / L the 5' end modified by fluorescein Strand DNA molecule B is then pumped into the sample pool at 300 μL / min (the pool is equipped with an evanescent wave optical fiber sensor probe whose surface is modified with single-stranded DNA molecule A shown in sequence 3 of the sequence listing), and the time is 30s, and further reaction 4min;

[0064] 3. Same as 3 in step 1 in embodiment 2...

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Abstract

The invention discloses a detecting method for divalent mercury ions. The principle for detecting mercury ions by using the method is as follows: a single-chain DNA (Deoxyribose Nucleic Acid) molecule B modified by fluorescein contains a sequence which is complementary with a sequence of a DNA catching needle (a single-chain DNA molecule A) fixed on the surface of a probe, and also contains a T-T base pair mismatch structure which can be combined with the divalent mercury ions. During detection, the single-chain DNA molecule B modified by the fluorescein and the mercury ions are simultaneously input to the surface of an optical fiber sensor probe for evanescent waves, and the divalent mercury ions are combined with the single-chain DNA molecule B modified by the fluorescein by the DNA catching needle in a competition way. The more the concentration of the divalent mercury ions is, the fewer the single-chain DNA molecule B modified by the fluorescein and combined with the DNA catching needle on the optical fiber sensor probe for evanescent waves is, so that a fluorescence intensity signal detected by an optical detecting platform for evanescent waves is weaker. An effective means is provided for the rapid on-site and real-time detection of heavy metal mercury ions in the fields of foods, medicines and environments.

Description

technical field [0001] The invention relates to a method for detecting divalent mercury ions. Background technique [0002] Mercury is a highly toxic heavy metal pollutant that widely exists in nature. Mercury may be contained in various media such as air, water, and soil. Mercury in the environment comes from natural releases and human activities. From the perspective of local pollution, anthropogenic sources are quite important, mainly including: smelting of mercury ore and other metals, incineration of solid waste, use in chlor-alkali industry and electrical industry, and combustion of fossil fuels. Mercury in the environment mainly exists in two forms: inorganic state and organic bonded state. Different forms of mercury have different toxicity, among which methylmercury is the most toxic. Mercury can enter the human body through breathing inhalation, skin adsorption, or food intake, causing brain and kidney damage, cell division disorders, and central nervous system da...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 龙峰
Owner BEIJING INST OF COLLABORATIVE INNOVATION
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