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Preparation method and application of stemona effective part extract

A technology of effective parts and extracts, which is applied in the field of preparation of extracts of effective parts of basil, can solve problems that have not yet been seen

Inactive Publication Date: 2013-11-13
LANZHOU UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no report about the preparation of anti-acetylcholinesterase drugs from the effective parts of C.

Method used

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  • Preparation method and application of stemona effective part extract
  • Preparation method and application of stemona effective part extract
  • Preparation method and application of stemona effective part extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Preparation of the effective part extract

[0016] (1) The preparation of the total alkaloid extract of Herba Melonifolia

[0017] Take 5kg of herbal medicines, crush them, add 10L of 95% ethanol to reflux and extract twice, each time for two hours, combine the extracts, concentrate to dryness, add 3L of distilled water to dissolve, adjust the pH value to 1-2 with 4M concentrated hydrochloric acid, and statically After 12 to 16 hours, filter with suction. The filtrate was adjusted to pH 9-11 with concentrated ammonia water or aqueous sodium hydroxide solution, and then extracted twice with 5 L of chloroform, and the extracts were combined and concentrated to dryness to obtain the total alkaloid extract (75 g).

[0018] (2) Purification of the total alkaloid extract of Herba Cinnamomi

[0019] After dissolving the above-mentioned total alkaloid extracts of Radix radicis with 100mL ethanol, pass through the macroporous resin column chromatography, use ethanol...

Embodiment 2

[0020] Embodiment 2: The modified Ellman method measures the acetylcholinesterase inhibitory activity of each eluted fraction after the macroporous resin column chromatography of the total alkaloids of the plant

[0021] The modified Ellman method was used to determine the inhibition rate of acetylcholinesterase by each elution fraction after macroporous resin column chromatography of the total alkaloids of Phetoperaceae, and Huperzine A was used as the positive control drug. The specific experimental process is as follows:

[0022] Use a 96-well microtiter plate to determine the inhibitory activity of the sample on the enzyme. Add 140 μL of 0.1M phosphate buffer (pH=8.0) to each well of the sample group, and then add 20 μL of the sample. Add 160 μL of phosphate buffer to the blank well without adding For any sample, 20 μL of 0.1 mg / mL physostigmine and 140 μL of phosphate buffer were added to the positive control control group. Add 15 μL of acetylcholinesterase, mix well and...

Embodiment 3

[0027] Embodiment 3: Morris water maze experiment

[0028] 70 healthy Kunming male mice were randomly divided into 7 groups: normal control group, model control group, high-dose total alkaloids group (1 mg / kg), low-dose total alkaloids group (0.5 mg / kg), F component High-dose group (1mg / kg), low-dose group of F component (0.5mg / kg) and donepezil control group (0.1mg / kg). Except the normal control group and the model control group were intraperitoneally injected with the same amount of normal saline, the mice in the other groups were injected intraperitoneally once a day at a fixed time for 5 consecutive days. After 2 days of administration, water maze positioning navigation training was carried out. Except for the normal control group, which was intraperitoneally injected with the same amount of normal saline, the mice in the other groups were intraperitoneally injected with 2 mg / kg of scopolamine before training. One hour after the last administration, positioning navigatio...

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Abstract

The invention relates to a preparation method and an application of a stemona effective part extract. According to the invention, stemona is subjected to reflux extraction by using 95% alcohol, such that an alcohol extract is obtained; the alcohol extract is dissolved by using water; the pH of the solution is regulated to 1-2 by using concentrated hydrochloric acid; the solution is filtered; the pH value of the filtrate is regulated to 9-11 by using concentrated ammonia water or a sodium hydroxide water solution; extraction is carried out by using a moderate polarity organic solvent; reduced-pressure concentration is carried out, such that a stemona total alkaloid extract is obtained; and the stemona total alkaloid extract is dissolved by using a small amount of ethanol; the solution is subjected to macroporous resin column chromatography; ethanol-water gradient elution is carried out; 50-80% ethanol-water elution part is collected; and the part is concentrated until dry, such that the stemona effective part extract is obtained. The application of the stemona effective part extract is the application in preparing an actylcholinesterase inhibitor.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the preparation and application of the extract of the effective part of the basilica. Background technique [0002] Alzheimer's disease (AD) is a chronic neurodegenerative disease characterized by memory impairment, cognitive dysfunction, behavioral disturbance and decline in self-care ability, and is the most common disease in senile dementia. The incidence of AD is positively correlated with age. Acetylcholinesterase (AChE) is a key enzyme in biological nerve conduction. In cholinergic synapses, this enzyme degrades acetylcholine, terminates the excitatory effect of neurotransmitters on the postsynaptic membrane, and ensures that nerve signals Normal transmission in living organisms. If the activity of this enzyme is inhibited, it can produce cholinergic effect. In recent years, clinical studies at home and abroad have shown that acetylcholinesterase inhibitors (actylcholinesterase i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/904A61P25/28A61P1/00A61P21/04A61P27/06
Inventor 杨中铎赖东海
Owner LANZHOU UNIVERSITY OF TECHNOLOGY
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