Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Alicyclobacillus immunomagnetic microspheres and application thereof

A Bacillus and magnetic microsphere technology, applied in the field of immunomagnetic microspheres, can solve the problems of high cost, increased production cost, decreased filtering effect, etc., and achieves the effects of low cost, good effect and fast speed

Active Publication Date: 2013-11-06
NORTHWEST A & F UNIV
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

High-temperature treatment will have a certain impact on the flavor of the juice, and the membrane filtration method is costly and the filtration effect decreases after a certain period of use, so the filter membrane needs to be replaced periodically according to the filtration effect, resulting in an increase in production costs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Alicyclobacillus immunomagnetic microspheres and application thereof
  • Alicyclobacillus immunomagnetic microspheres and application thereof
  • Alicyclobacillus immunomagnetic microspheres and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (1) Silanization reaction of magnetic microspheres

[0045] Dissolve 9.5g of sodium silicate in 200mL of deionized water and adjust the pH value to 12-13 with hydrochloric acid, add 5g of Fe to the sodium silicate solution 3 o 4 Ultrasonic treatment with magnetic core for 30 minutes, adjust the pH of the reaction system to 6-7 with hydrochloric acid in a water bath at 80-90°C, obtain microspheres by magnetic separation and wash with water to obtain silanized magnetic microspheres with a neutral pH;

[0046] (2) Amination modification of magnetic microspheres

[0047] Add 2 grams of silanized magnetic microsphere suspension, 1mL deionized water and 10mL APTES to 150mL methanol, add 150mL glycerin after ultrasonic treatment for 30min, fully react at 85-90℃ for 3h, obtain microspheres by magnetic separation, and use methanol and deionized water Washing three times respectively, freeze-drying to obtain aminated magnetic microspheres, and the particle diameter of the amina...

Embodiment 2

[0053] This example differs from example 1 in that the Fe used in this example 3 o 4 The magnetic core is prepared by the following method:

[0054] 3.4g of FeCl 3 ·6H 2 O with 1.2g FeCl 2 4H 2 O was added to 800mL deionized water, and 4g PEG-4000 was added, fully stirred and dissolved under the protection of nitrogen, and ammonia solution was added dropwise to make the pH of the reaction system greater than 10; after that, the reaction system was fully matured in a water bath at 75-85°C for 20-40min , the microspheres were obtained by magnetic separation and washed with water to obtain Fe 3 o 4 Magnetic nucleus; the Fe was detected 3 o 4 The magnetic core particle size is 15-25nm, and the magnetic saturation magnetization is 72.18emu / g.

Embodiment 3

[0056] The difference between this example and Example 1 is that the immunomagnetic microspheres of this example are blocked by a blocking solution: use a mixed system containing 5g BSA and 0.05ml Tween-20 per 100ml of PBS as the blocking solution, and add the adsorbed antibody The immunomagnetic microspheres were reacted at 37° C. for 60 min, and then magnetically separated and fully washed to obtain the immunomagnetic microspheres after blocking treatment.

[0057] One, utilize the immunomagnetic microsphere prepared in embodiment 3 to separate Alicyclobacillus:

[0058] The specific operation steps are: prepare 10 mL of the concentration gradient samples shown in Table 1, add 20 mg of immunomagnetic microspheres to each sample to separate and remove the target bacteria: 120-180 rpm, reaction adsorption time 60 min; magnetic separation and adsorption of bacteria The immunomagnetic microbeads were washed with sterile water and suspended in 200 μL of PBS buffer. The change of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Saturation magnetizationaaaaaaaaaa
Login to View More

Abstract

The invention discloses alicyclobacillus immunomagnetic microspheres and an application thereof. The disclosed alicyclobacillus immunomagnetic microspheres are obtained by coating aminated magnetic microspheres with oxidized alicyclobacillus specific antibodies. A preparation method of the oxidized alicyclobacillus specific antibodies comprises the following steps: alicyclobacillus specific antibodies are oxidized by using a PBS solution of sodium persulfate, and then the oxidized alicyclobacillus specific antibodies are collected to obtain. The disclosed alicyclobacillus immunomagnetic microspheres can be used for effectively separating alicyclobacillus in fruit juice especially in apple juice.

Description

technical field [0001] The invention belongs to the technical field of food microorganism detection, and in particular relates to immunomagnetic microspheres of Alicyclobacillus and applications thereof. Background technique [0002] Alicyclobacillus, commonly known as acidophilic and thermostable bacteria, belongs to Gram-positive bacillus, rod-shaped, spore-forming, strictly aerobic, and can survive and grow in a wide temperature range of pH 2.5-6.0 and 20-60°C , is a common contaminating bacteria in apple and other fruit juices. Due to its heat resistance and acid resistance, ordinary pasteurization is difficult to kill it. The bacterium does not multiply in concentrated fruit juice, but when the concentrated fruit juice is diluted into low-concentration fruit juice, it can metabolize and produce substances with unpleasant flavors - guaiacol and 2,6-dibromophenol, which make the taste and flavor of the juice worse , forming a white precipitate or foggy turbidity, which ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68A23L3/3526
Inventor 岳田利王周利袁亚宏蔡瑞牛晨郭彩霞
Owner NORTHWEST A & F UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com