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Silk fibroin nanofiber membrane and preparation method thereof

A technology of nanofiber membrane and silk fibroin, applied in the field of silk fibroin nanofiber membrane and its preparation, can solve the problems of poor mechanical properties of silk fibroin nanofiber membrane and damage to nanostructure, etc. Fast, excellent biocompatibility results

Active Publication Date: 2013-10-09
上海丝波敦生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] On the other hand, inside the natural silk, there is a wire structure with a diameter of tens of nanometers in the silk gland before silkworm spinning, and this nanostructure plays a key role in the formation of silk and the excellent mechanical properties of silk; however, the existing technology In the natural silk regeneration process, the dissolution step severely damaged the nanostructure, which became one of the main reasons for the poor mechanical properties of the regenerated silk fibroin nanofiber membranes.

Method used

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  • Silk fibroin nanofiber membrane and preparation method thereof
  • Silk fibroin nanofiber membrane and preparation method thereof
  • Silk fibroin nanofiber membrane and preparation method thereof

Examples

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Effect test

Embodiment 1

[0037] (1) Natural mulberry silk was boiled with 0.5wt% sodium carbonate solution for 30 minutes, degummed, and degummed mulberry silk was obtained after repeating 3 times;

[0038] (2) Put the degummed mulberry silk in 95wt% formic acid containing 5wt% lithium bromide at a concentration of 20wt%, stir and dissolve at room temperature for 2 hours, then pour it into a polyethylene plate to dry to form a film;

[0039] (3) Soak the membrane in step (2) in running water for 3 hours to remove lithium bromide, and then dry it in a vacuum oven at 40°C for 24 hours;

[0040] (4) dissolving the membrane in step (3) in 80wt% formic acid to prepare 5wt% and 8wt% spinning solutions, and then electrospinning to obtain nanofiber membranes;

[0041] (5) The nanofiber membrane obtained in step (4) is left for 48 hours, or directly soaked in ethanol solution for 30 minutes to obtain a silk fibroin nanofiber membrane.

[0042] attached figure 1 It is the scanning electron micrograph of the a...

Embodiment 2

[0044] (1) Natural mulberry silk was degummed by boiling 0.5wt% sodium carbonate solution for 30 minutes, and degummed mulberry silk was obtained after repeating 3 times;

[0045](2) Put degummed mulberry silk at a concentration of 10wt% in 50wt% mixed acid containing 5wt% lithium thiocyanate (phosphoric acid: formic acid = 1:4, mass ratio), stir and dissolve at 60°C for 1 hour, and then pour it into poly Dried into a film on a vinyl plate;

[0046] (3) Soak the membrane in step (2) in running water for 3 hours to remove salt and residual acid, and then dry it in a vacuum oven at 60°C for 20 hours;

[0047] (4) Dissolve the film in step (3) in hexafluoroisopropanol to prepare a 10wt% solution, and dissolve chitosan in hexafluoroisopropanol to prepare a 10wt% solution, and then mix the two solutions at a ratio of 1:1 Volume blending preparation concentration is 10wt%, silk fibroin and chitosan 1:1 (mass ratio) blended spinning solution;

[0048] (5) Process the blended spinni...

Embodiment 3

[0051] (1) Natural mulberry silk was degummed by boiling 0.5wt% sodium carbonate solution for 30 minutes, and degummed mulberry silk was obtained after repeating 3 times;

[0052] (2) Put the degummed mulberry silk in 80wt% formic acid containing 10wt% zinc chloride at a concentration of 10wt%, stir and dissolve at room temperature for 2 hours, and then pour it into a polyethylene plate to dry to form a film;

[0053] (3) Soak the silk fibroin film in step (2) in flowing water for 3 hours to remove zinc chloride and residual acid, and then dry it in a vacuum drying oven at 80°C for 15 hours;

[0054] (4) dissolving the membrane in step (3) in 80wt% formic acid to prepare an 8wt% spinning solution, and then electrospinning to obtain a silk fibroin nanofiber membrane;

[0055] (5) The silk fibroin nanofiber membrane obtained in step (4) was treated in 80 wt% methanol for 30 minutes to realize the transformation of the silk fibroin structure from amorphous to β-sheet.

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Abstract

The invention discloses a silk fibroin nanofiber membrane and a preparation method of the silk fibroin nanofiber membrane. The preparation method comprises the following specific steps of: dissolving natural silk taken as a main raw material by an acid salt solution, forming a membrane, desalting the membrane, dissolving the membrane by methanoic acid and hexafluoroisopropanol to prepare a spinning solution, and carrying out electrostatic spinning to prepare the silk fibroin nanofiber membrane. The silk fibroin nanofiber membrane comprises fibers with the diameter of 10nm-10 microns and has the excellent mechanical property, the breaking strength of greater than 8Mpa at dry state, the breaking elongation of greater than 15% at dry state, the breaking strength of greater than 1Mpa at wet state and the breaking elongation of greater than 100% at wet state. In addition, the silk fibroin nanofiber membrane prepared by the method is stable and controllable in structure, has good biocompatibility and can serve as a medicinal biological material. The preparation method disclosed by the invention is simple and short in flow path, has high film formation and spinning efficiency and is suitable for industrialization large-scale production.

Description

technical field [0001] The invention relates to a nanofiber membrane and a preparation method thereof, in particular to a silk fibroin nanofiber membrane and a preparation method thereof. The method is simple and efficient and can be widely used in the fields of biology and medicine. Background technique [0002] my country is the main producer of silk, and its silk output accounts for more than 70% of the world's output. In recent years, the research and application of silk has extended from the traditional textile field to high-tech fields, such as optoelectronics and biomedical materials, especially as biomedical materials have made important progress. Silk fibroin is obtained by degumming silk from silkworm cocoons. It is a natural structural protein with no physiological activity. It is mainly composed of three simple amino acids--glycine, alanine and serine, which account for about 85% of the total protein. . The degummed silk has good biocompatibility and mechanical...

Claims

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Application Information

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IPC IPC(8): A61L27/22
Inventor 左保齐吕强张锋
Owner 上海丝波敦生物科技有限公司
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