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Arabidopsis thaliana heat shock transcription factor gene HSFA5, its encoded protein and application

A technology of heat shock transcription factor and Arabidopsis thaliana, which is applied in the field of plant molecular biology and transgenic, and can solve the problems of increasing the difficulty of gene transgenic application

Inactive Publication Date: 2013-09-11
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

But so far there is no report about the heat stress response of HSFA5 gene, which increases the difficulty of understanding the function of the gene and its transgenic application

Method used

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  • Arabidopsis thaliana heat shock transcription factor gene HSFA5, its encoded protein and application
  • Arabidopsis thaliana heat shock transcription factor gene HSFA5, its encoded protein and application
  • Arabidopsis thaliana heat shock transcription factor gene HSFA5, its encoded protein and application

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Embodiment Construction

[0014] The present invention will be further described below in conjunction with experimental methods and data.

[0015] 1. Arabidopsis seedling culture:

[0016] Wild-type and mutant seeds were sterilized by soaking in 0.5% sodium hypochlorite solution for 10 min, and the sterilized seeds were placed in a 4°C refrigerator for vernalization for 2 days, and then spread on MS (Murashige and Skoog) solid medium containing 1% agar. Germinate and grow in a light incubator at 22°C.

[0017] 2. Homozygous mutant identification:

[0018] DNA extraction: All experimental drugs used were purchased from Zhongke Ruitai Bioengineering Co., Ltd. Take about 0.5g fresh leaves of Arabidopsis thaliana, grind them into a paste, add 400μl SDS extract (0.5% SDS (W / V); 200mM Tris-HCl, pH=8.0; 250mM NaCl; 25mM EDTA); centrifuge at 12,000r / min 3min; take the supernatant, add an equal volume of isopropanol to gently shake, let stand for 30min; centrifuge at 13,000r / min for 5min; discard the superna...

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Abstract

The invention belongs to plant molecular biology and transgenosis related technical field, and specifically relates to verification of the high temperature resistance function of the Arabidopsis thaliana heat shock transcription factor gene HSFA5, and application of the gene in breeding new varieties of high temperature resistant plants. In the invention, the model plant Arabidopsis thaliana is adopted as the material, and T-DNA of the HSFA5 gene is inserted into a homozygous mutant strain salk_004385 to study the function of the HSFA5 gene in the aspect of plants' high temperature resistance. Experimental results show that: the HSFA5 gene knockout mutant shows a heat resistant phenotype under heat shock stress, and its survival rate is significantly higher than that of a wild type; and the HSFA5 gene plays an important role in a signal transduction pathway of plants' stress response to heat shock stress, and it is estimated that overexpression of the gene can improve heat resistance of plants and has great economic value in molecular breeding of plant stress resistance.

Description

Technical field: [0001] The invention relates to the field of plant molecular biology and transgenic technology, specifically the verification of the new function of HSFA5, a member of the Arabidopsis thaliana heat shock transcription factor (Heat Shock Transcription Factors, HSFs) gene family, for high temperature resistance, and the application of the gene in new high temperature resistant plant varieties applications in cultivation. Background technique: [0002] The change of temperature has an important impact on the growth and development of plants. Appropriate temperature can ensure or promote the normal growth and development of plants, while unsuitable temperature conditions will inhibit the growth of plants and even cause plant death. Since the 20th century, with the rapid growth of world population and the continuous deepening of industrialization, the greenhouse effect has become more and more serious, and the gradual increase of global temperature has become an ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415A01H5/00
Inventor 徐江徐小栋鲁黎明王西瑶张少斌东方阳迟志海洪雪麻艳超赵明丁在松付金东
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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