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Related protein capable of adjusting translation efficiency of chloroplast protein and improving heat resistance of plants, and application thereof

A chloroplast and plant technology, applied in the fields of botany and biology, can solve the problems of undiscovered chloroplast protein translation efficiency, plant heat-resistant thylakoid membrane heat-resistant proteins, etc.

Inactive Publication Date: 2013-09-11
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] So far, no heat-resistant protein that can regulate the translation efficiency of chloroplast proteins, improve plant heat tolerance and maintain thylakoid membrane stability has been found in the art

Method used

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  • Related protein capable of adjusting translation efficiency of chloroplast protein and improving heat resistance of plants, and application thereof
  • Related protein capable of adjusting translation efficiency of chloroplast protein and improving heat resistance of plants, and application thereof
  • Related protein capable of adjusting translation efficiency of chloroplast protein and improving heat resistance of plants, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Identification of rps1 T-DNA mutants

[0088] The inventor purchased the rps1 T-DNA mutant from the Salk website, and in the current planting period, the rps1 T-DNA mutant isolated plants with yellow leaves. According to Salk's prediction, the T-DNA insertion site of rps1 is in the first exon of the RPS1 gene.

[0089] Based on the sequence information, the inventors synthesized the identification primer sequence of RPS1 (CS874869) T-DNA insertion mutant:

[0090] LP(874869-L): 5'-GACTCCAGCTGGTTTAGAGGG-3' (SEQ ID NO.: 4);

[0091] RP(874869-R): 5'-CAAGTAAGCCGATGACTTTGC-3' (SEQ ID NO.: 5);

[0092] LB1: 5'-GCCTTTTCAGAAATGGATAAATAGCCTTGCTTCC-3' (SEQ ID NO.: 6); and

[0093] LB2: 5'-GCTTCCTATTATATCTTCCCAAATTACCAATACA-3' (SEQ ID NO.: 7).

[0094] Use the above primers as a primer pair, and use the genomic DNA of the rps1 T-DNA mutant as a template to perform a PCR reaction to identify the T-DNA insertion position in the rps1 mutant (the PCR product is connected to the T...

Embodiment 2

[0106] Downregulation of RPS1 expression reduces the stability of the thylakoid membrane system in a dose-dependent manner

[0107] 1. Thylakoid stacking degree determination method: reference (Khatoon et al., 2009), with appropriate modifications:

[0108] 1). Take 3-4g leaves of Arabidopsis thaliana, add 20ml ice-cold GB buffer solution, and quickly grind it in a pre-cooled mortar until it is homogenized;

[0109] 2). The homogenate is filtered with dust-free paper (2-4 layers);

[0110] 3). The filtrate was centrifuged at 4°C, 2600g, for 3min;

[0111] 4). Discard the supernatant, add 10ml RB buffer, and resuspend;

[0112] 5). Centrifuge at 2600g for 3min at 4°C;

[0113] 6). Repeat steps 4 and 5 once;

[0114] 7). Resuspend the precipitate in Solution C, measure the chlorophyll concentration with the acetone method, and adjust the chlorophyll concentration to 0.25mg / ml with Solution C;

[0115] 8). Incubate at 4°C for 15 minutes in the dark;

[0116] 9). Add digiton...

Embodiment 3

[0126] To construct an estrogen-inducible RPS1-RNAi binary vector, the construction method is as follows:

[0127] The sequence between 25 bp upstream and 457 bp downstream of the initiation codon ATG of RPS1 was amplified by RT-PCR.

[0128] The sense fragment primers are:

[0129] Upstream primer: 5′AAACCCGGGCTCGAGCTGTGTGAGTGAGTGAGACTC-3′

[0130] (SEQ ID NO.: 14)

[0131] Downstream primer: 5′-CGCTCTAGATGACAAACTCTTCCACCATAC-3′

[0132] (SEQ ID NO.: 15)

[0133] Antisense fragment primers are:

[0134] Upstream primer: 5′-AAAGAGCTCCTCGAGCTGTGTGAGTGAGTGAGACTC-3′

[0135] (SEQ ID NO.: 16)

[0136] Downstream primer: 5′-CACGCGGCCGCTGACAAACTCTTCCACCATAC-3′

[0137] (SEQ ID NO.: 17)

[0138] PCR conditions: annealing at 54°C for 15s, extension at 72°C for 0.5min, 30 cycles.

[0139] The amplified sense fragment and antisense fragment were connected to the commercially available pMD19-T vector by TA cloning method, and then transferred into the commercially available host...

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Abstract

The invention relates to related protein capable of adjusting translation efficiency of chloroplast protein and improving heat resistance of plants, and application thereof. In particular, the inventor screens one mutant rps1 related to the translation efficiency of the chloroplast protein and the heat resistance of the plants from various different arabidopsis mutants, and identifies a plant chloroplast ribosome small subunit S1 (RPS1) gene from the mutant; and the gene and polypeptide can effectively maintain the heat stability of a thylakoid membrane, can improve the translation efficiency of the chloroplast protein of the plants, can improve the survival ability and the photosynthesis efficiency of the plants under heat stress, and can effectively avoid and reduce adverse influence of high temperature on the plants.

Description

technical field [0001] The invention belongs to the fields of botany and biotechnology. Specifically, the invention relates to a related protein that regulates the translation efficiency of chloroplast protein and improves the heat tolerance of plants and its application. Background technique [0002] Due to the increase of carbon dioxide emissions, the earth's greenhouse effect continues to intensify, leading to global warming. Plants growing in a natural environment are affected by rising temperature, which hinders the growth of plants, especially some spring crops, such as rice, corn, etc., are easily affected by high temperature weather during the earing and filling period, causing crops production cuts. According to the data of the International Rice Research Institute, from 1998 to 2003, every 1°C increase in temperature will reduce the global crop production by 10%. According to experts' prediction, the global temperature may rise by 5-6°C in the next 100 years. In ...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/84A01H5/00
Inventor 郭房庆于海东杨小飞
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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