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Streptomy cesgriseochromogenes for preventing tobacco wildfire

A technology for Streptomyces griseochromogenes and tobacco wildfire disease, applied in the direction of chemicals for biological control, methods based on microorganisms, bacteria, etc., can solve the unreasonable use of technology, aggravate the pollution of the pesticide environment and the destruction of the ecosystem , wildfire pathogenic strains and other problems, to achieve good control effect

Inactive Publication Date: 2013-07-03
SOUTHWEST UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extensive and long-term continuous use of chemical pesticides in tobacco production and the unreasonable use of technology have resulted in the resistance of tobacco wildfire pathogens, and there are differences in the resistance of wildfire pathogen strains from different sources
The emergence of drug-resistant strains not only increases the cost of disease control, but also aggravates the environmental pollution and ecosystem damage caused by the use of pesticides, and also directly affects the quality of tobacco leaves

Method used

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  • Streptomy cesgriseochromogenes for preventing tobacco wildfire
  • Streptomy cesgriseochromogenes for preventing tobacco wildfire
  • Streptomy cesgriseochromogenes for preventing tobacco wildfire

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1. Identification of Streptomyces grisea chromogenes TYFCQ272 bacterial strain

[0036] 1.1 Observation of colony characteristics ： Yeast refined maltose agar medium (ISP-2), oat flour agar medium (ISP-3), inorganic salt starch agar medium (ISP-4), glycerol asparagine agar medium (ISP-5) and potato Inoculate juice medium, Czapck medium and Gao's No. 1 medium on the above 7 kinds of medium plates by streaking and inoculating at 28°C for 7-14 days. After the mycelium grows stably, use The dissecting microscope was used to observe the single colony morphology, aerial hyphae, basal hyphae, and sporocystium color.

[0037] 1.2 Observation of characteristics of mycelia and spores ：The morphology and spore characteristics of the fungi to be tested were observed by the insert method. Take the suspension of the bacteria to be tested and spread it on Gaoshi No. 1 medium, pick up the sterilized cover glass with tweezers, insert it into the medium at an angle of about...

Embodiment 2

[0041] Embodiment 2. Disease control effect of Streptomyces griseochromogenes TYFCQ272 bacterial strain

[0042] 2.1 Antibacterial effect of strain TYFCQ272: inoculate the strain on the center of the PDA plate, incubate at 28°C for 3 days, then put the filter paper dipped in chloroform into the bottom of the inverted petri dish and fumigate for 20 minutes to kill TYFCQ272 bacteria. Then use monkey head sprayer to spray 3×10 8 Spray the cfu / mL P. tobacco suspension on the plate (about 100 μL / dish), repeat 3 times. After culturing at a constant temperature of 28°C for 48 hours, measure the diameter of the inhibition zone. To determine the inhibitory effect of the strain TYFCQ272 on the tobacco wildfire pathogen.

[0043] 2.2 Disease control effect of strain TYFCQ272: 4 treatments were set up. 1: Use spray inoculation, spray the antagonistic bacteria suspension until the leaves are covered with fog spots, and keep moist for 24 hours after inoculation; 2: Spray the antagonistic...

Embodiment 3

[0045] Example 3. Analysis of metabolites of bacterial strain TYFCQ272

[0046] 3.1 Detection of chitinase activity

[0047] Sole carbon source medium (Chi-Ayers): 1.0g ammonium dihydrogen phosphate, 0.2g magnesium sulfate, 0.2g potassium chloride, 20g agar, 1% colloidal chitin to 1000mL, pH: 7.0.

[0048] Preparation of colloidal chitin: Dissolve 20g of chitin in 350mL of concentrated hydrochloric acid, place at 4°C for 24h, filter through glass wool, add 2L of ice-free ethanol to the filtrate overnight at -20°C, centrifuge at 10,000 r / min for 20min, rinse with tap water Precipitate to neutral pH, freeze-dry, and store in a sealed seal at -20°C. When using colloidal chitin, it must be ground more than 5 times with a manual homogenizer.

[0049] Inoculate the antagonistic bacteria on the plate made of the above medium, culture at 28°C for 7-10 days, observe whether there is a transparent circle and measure the size of the transparent circle, if there is a transparent circle,...

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Abstract

The invention relates to a streptomy cesgriseochromogenes strain TYFCQ272 for preventing tobacco wildfire, and belongs to the field of biological control of agricultural diseases and insect pests. The strain is separated from the host plant rhizosphere soil in natural environment, and named streptomy cesgriseochromogenes according to the morphological characteristics and molecular biological identification. The strain has a strong inhibition effect for tobacco wildfire, and has a good control effect for tobacco wildfire. Meanwhile, the strain also has broad-spectrum antagonistic activity, and has better antibacterial effects for tobacco gray mold, apple canker, thielaviopsis basicola and other pathogenic fungi, the radius of each inhibition zone is 10.0 mm above, the strain also has stronger antibacterial effects for citrus canker, pseudomonas angulata and other pathogenetic bacteria, and the radius of each inhibition zone is above 20.0 mm. The secondary metabolites of the strain include cellulase, protease, siderophores, and the like. The TYFCQ272 cell preparation or the bacteria-free filtrate has good control effect for tobacco wildfire, the environment-friendly biological pesticide can be produced by cell fermentation or the secondary metabolites extraction technology, therefore, the streptomy cesgriseochromogenes has important commercial development and application values.

Description

technical field [0001] The invention relates to the technical field of biological control of plant diseases, in particular to a strain of Streptomyces chromogenes used for preventing and treating tobacco wildfire disease. Background technique [0002] Tobacco wildfire disease is an important disease that commonly occurs in various tobacco producing areas in the world. It is caused by Pseudomonas tobacco, Pseudomonas syringae pathogenic species Pseudomonas syringae pv. tabaci )caused. The disease was first reported by Wolf and Foser (1917), and has since been reported in many countries such as the former Soviet Union, the United States, Germany, Japan, and Brazil. Since the 1980s, the disease has risen as a major disease in my country, and it has become one of the major diseases in tobacco production in three northeastern provinces, Sichuan, Henan, Yunnan, Guizhou, and Chongqing. The disease mainly damages the leaves, especially in the middle and late stages, seriously a...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01P1/00C12R1/465
Inventor 马冠华肖崇刚董国菊邢小军王勇卢军
Owner SOUTHWEST UNIVERSITY
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