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Preparation method and application of electrochemical micro-fluidic sensing chip

A sensor chip and microfluidic technology, applied in chemical instruments and methods, scientific instruments, laboratory containers, etc., can solve the problems of poor repeatability of electrochemical detection, complicated preparation process, high cost, etc., and achieve ultra-high detection Sensitivity and accuracy, detection repeatability, light weight effect

Active Publication Date: 2013-07-03
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, the current preparation of electrochemical microfluidic sensor chips is to process metal wires as electrodes into microfluidic chips, such as the microfluidic electrochemical detection device reported by James F. Rusling (Electrochemistry Communications11 (2009) 819–822) , is to use a gold wire with a diameter of 0.5mm. After being treated with aqua regia, the surface is further modified and then processed onto a microfluidic chip. The preparation process of this device is complicated, time-consuming and costly, and it needs to be completed in a clean room. , the general laboratory conditions are difficult to achieve, and its electrochemical detection repeatability is poor

Method used

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  • Preparation method and application of electrochemical micro-fluidic sensing chip
  • Preparation method and application of electrochemical micro-fluidic sensing chip
  • Preparation method and application of electrochemical micro-fluidic sensing chip

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Experimental program
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Effect test

Embodiment 1

[0043] (a) Using Freehand drawing software to design the microfluidic pipeline drawing mask, the pipeline design is based on the fluid mechanics design such as figure 1 As shown, the inlet and outlet are designed with a certain radian, and there is no right angle. The middle of the pipe is oval to ensure that the liquid passes smoothly and evenly across the surface of the working electrode. This technology is different from other microfluidic chips that usually use rectangular pipe design.

[0044] (b) Fabrication of PDMS chips using soft lithography standard microfabrication techniques.

[0045] (c) Preparation of glass solution, 3-aminopropyltriethoxysilane (APTS), ethyl orthosilicate (TEOS), ethanol (Et OH), water (H 2 O pH=2–8) mixed at a volume ratio of 1:1:1:1, ultrasonicated for 5–30 minutes, and aged at 20-90°C for 2–24 hours. , For example, the ultrasound can be 5min, 15min, 30min, the curing temperature can be 20°C, 50°C, 90°C, the curing time can be 2h, 15h, 24h an...

Embodiment 2

[0054] (a) Use the Freehand drawing software to design the microfluidic pipeline drawing mask, the specific design is shown in the appendix figure 1 .

[0055] (b) Fabrication of PDMS chips using soft lithography standard microfabrication techniques.

[0056] (c) Preparation of glass solution, 3-aminopropyltriethoxysilane (APTS), ethyl orthosilicate (TEOS), ethanol (Et OH), water (H 2 O pH=2-8) mixed according to the volume ratio of 5:1:1:1 to 1:5:10:20, ultrasonicated for 5-15min, and aged at 20-90℃ for 2-24h.

[0057] (d) Apply a layer of glass solution evenly on the surface of the standardized printed electrode, and let it dry at room temperature.

[0058] (e) A PDMS chip and a glass-coated printed electrode O 2 Plasma treatment for 60s, then bonding.

[0059] (f) Immobilize 50 μL of 0.05-0.5 mg / m magnetic beads with CEA capture antibody to the working area of ​​the electrode of the chip under the action of an external magnetic field.

[0060] (g) Inject 100 μL of 0.1 ...

Embodiment 3

[0065] (a) Use the Freehand drawing software to design the microfluidic pipeline drawing mask, the specific design is shown in the appendix figure 1 .

[0066] (b) Fabrication of PDMS chips using soft lithography standard microfabrication techniques.

[0067] (c) Preparation of glass solution, 3-aminopropyltriethoxysilane (APTS), ethyl orthosilicate (TEOS), ethanol (Et OH), water (H 2 O pH=2-8) mixed at a volume ratio of 1:5:10:20, ultrasonicated for 5-20min, and aged at 20-90°C for 2-24h.

[0068] (d) Apply a layer of glass solution evenly on the surface of the standardized printed electrode, and let it dry at room temperature.

[0069] (e) A PDMS chip and a glass-coated printed electrode O 2 Plasma treatment for 90s, followed by bonding.

[0070] (f) Immobilize 50 μL of 0.05–0.5 mg / m magnetic beads with CA199 capture antibody to the working area of ​​the chip electrode under the action of an external magnetic field.

[0071] (g) Inject 100 μL of 10 ng / mL CA199 antigen i...

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Abstract

The invention provides a preparation method and the application of an electrochemical micro-fluidic sensing chip. The preparation method comprises the following steps: directly coating an improved glass solution on a commercial standard printed electrode; and performing vacuum plasma treatment on a PDMS (Polydimethylsiloxane) chip with pre-designed pipelines and the printed electrode coated with the glass solution together, and directly bonding the PDMS chip on the commercial standard printed electrode to form a novel electrochemical microfluidic sensing platform. A sensor provided by the invention can perform ultrasensitive detection on various sample analytes in a biological fluid sample, taking the detection of a prostate cancer marker PSA (Prostate-specific Antigen) in human serum as an example, a coulomb amperometry is used for detection, and a result shows that the detection sensitivity can reach 0.84 pg / mL which is improved by two magnitudes than the standardized clinical testing requirement of 0.1 ng / mL, so that the sensor has superhigh detection sensitivity and accuracy, which are higher than those of other electrochemical detection devices, is convenient in operation, and can integrates sample processing, separation and the like on one micro electrochemical microfluidic sensing chip.

Description

technical field [0001] The invention relates to a microfluidic sensor chip, in particular to a preparation method and application of an electrochemical microfluidic sensor chip. Background technique [0002] The microfluidic system is a micro-volume liquid (10 -9 –10 -18 L) The process of manipulation in pipes of tens to hundreds of microns, this technology has very broad application prospects in biomedicine, environmental monitoring, and food safety. Microfluidic devices have the following advantages, such as small size, reduced reagent consumption, parallel detection of multiple samples, increased reliability, and sensitivity. The electrochemical system can be easily integrated into the microfluidic chip. Compared with traditional analysis platforms such as mass spectrometry and optical detection, electrochemical microfluidics has more flexible sample processing, superior sensitivity and versatility, without the aid of Huge optical inspection equipment. [0003] Howeve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N27/416
Inventor 陈守慧王智华聂志鸿陈小元崔大祥
Owner SHANGHAI JIAO TONG UNIV
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