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Composite nano-grade novel material based on cancer early-stage integrated detection, diagnoses, and treatment, and preparation method thereof

A technology for early detection and new materials, applied in the field of nanoscience, can solve the problems of low sensitivity and specificity, difficult detection, drug resistance, etc., and achieve the effects of high sensitivity and accuracy, improved accuracy, and stable performance

Inactive Publication Date: 2013-06-19
SHENYANG POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sensitivity and specificity of the current conventional diagnostic methods are relatively low, it is difficult to detect in the early stage of tumor occurrence, there are also poor targeted delivery effect, strong toxic side effects and drug resistance of tumor cells, etc., it is difficult to achieve the expected therapeutic effect

Method used

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  • Composite nano-grade novel material based on cancer early-stage integrated detection, diagnoses, and treatment, and preparation method thereof
  • Composite nano-grade novel material based on cancer early-stage integrated detection, diagnoses, and treatment, and preparation method thereof
  • Composite nano-grade novel material based on cancer early-stage integrated detection, diagnoses, and treatment, and preparation method thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0041] A method for preparing a new composite nano material based on the integration of early detection, diagnosis and treatment of cancer as described above is characterized in that: the specific steps are as follows:

[0042] (1) Take the silver nanocube as a template and add AuCl 2 - And AuCl 4 - , The hollow gold nanocube-shaped particles (nanoboxes) and hollow porous gold nanocage-shaped particles (nanocages) were prepared, and the hollow gold nanocube-shaped particles were used H 2 O 2 Corrosion to obtain angled hollow gold nanocubic box-shaped particles, such as figure 1 Not in

[0043] (2) Add 0.02 mol FeCl to the hollow gold nanocubic box particles and hollow porous gold nano cage particles obtained in step (1) 2 ·6H 2 O dissolved in 200mL deionized water and FeCl 3 ·4H 2 O, Fe 3+ With Fe 2+ The molar ratio is 1.75, and the reaction precursor solution is stirred rapidly, and ultrasonically oscillated to ensure Fe 2+ And Fe 3+ The solution can enter the hollow gold nanocage o...

Embodiment 1

[0066] Take Au Nanocages / Fe 3 O 4 As an example to prepare biological probes

[0067] (1) Reference literature [Chinese Sci Bull, 2010, 55] Preparation of Nano Fe 3 O 4 For particles, refer to the literature [Adv Mater, 2008, 20] to prepare gold nanocages or gold nanocube boxes.

[0068] (2) Take (1) 2g 10mmol of Au Nanocages particles and add 0.02mol FeCl 3 ·6H 2 O is dissolved in 200mL deionized water to make a 0.1mol / L solution, Fe 3+ With Fe 2+ The molar ratio is 1.75. When the reaction starts at 60°C, first add 30mL of ammonia water, and then add 0.5mL of surfactant when the temperature is raised to 80°C. The aging time is 40~130min; the stirring speed is close to 300r / min to obtain composite nanoparticles .

[0069] (3) At room temperature, add 1.5 mL of 20% PDDAC aqueous solution into 5 mL of deionized water and stir quickly, then slowly add the 7nM Au Nanocages / Fe prepared in (2) 3 O 4 The solution is 5mL, after continuing to stir for two hours, centrifuge at 15000rpm for 30 ...

Embodiment 2

[0074] Synthesis of 2,3-dimercaptosuccinic acid (DMSA) modified nanocomposites

[0075] (1) Reference [J Colloid Interface Sci. 1997, 194: 427-433] method, add 1mL DMSA solution (3mM) to 1.5mL nanocomposite colloidal solution (7.0×10 12 particles / mL), slightly stirred for 2 hours to obtain DMSA-modified composite nanomaterials.

[0076] (2) Centrifugally wash (1) with Milli-Q water to remove unreacted DMSA (3000 rpm). The nanomaterials were re-dispersed in 3 mL of aqueous solution for later use.

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Abstract

The invention provides a composite nano-grade novel material based on cancer early-stage integrated detection, diagnoses, and treatment, and a preparation method thereof. According to the invention, gold nano-cage (gold nano-box) and nano-grade iron oxide are assembled, such that gold nano-cage (box) / nano-grade iron oxide composite particles are synthesized. With an assembling method from the inside to the outside, the gold nano-cage (box) is first prepared; iron oxide is nucleated and grown in the gold nano-cage (box); with a surfactant (such as glucan or the like), mono-dispersed iron oxide is obtained; the iron oxide is packaged by using biological molecules, such that the gold nano-cage (box) / nano-grade iron oxide composite particles are obtained; surface modification is carried out by using biological targeting molecules (antibodies or DNA molecules). The material is used in tumor magnetic resonance imaging, fluorescence imaging, dark field imaging, specific targeting multifunctional diagnosis, and photothermal therapy. The material can also be used as a targeting medicine carrier.

Description

Technical field: [0001] The invention mainly relates to a new composite material based on the integration of early detection, diagnosis and treatment of cancer-gold nano cage / nano iron oxide, which belongs to the field of nano science and technology. Background technique: [0002] Biologically active substances such as antibody proteins, etc., are difficult to quantitatively analyze or detect due to relatively weak self-detectable signals. Cancer is a common and frequently-occurring disease that seriously endangers human health. According to the World Health Organization, there are more than 10 million new cancer cases and more than 7 million deaths worldwide every year, including 5.3 million men and 4.7 million women. Accounted for 12% of the total deaths. It is expected that by 2020, there will be 15 million new cases worldwide each year. The total number of cancer patients will increase by 73% in developing countries and 29% in developed countries. Cancer is one of the mai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/06A61K41/00A61K47/02B82Y5/00B82Y40/00A61P35/00
Inventor 杨玉东
Owner SHENYANG POLYTECHNIC UNIV
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