Effective Ustilaginoidea virens separation method
A technology of rice smut fungus and separation method, applied in the field of microorganisms, to achieve the effects of ensuring germination ability, avoiding killing effect, and weakening interference effect
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Embodiment 1
[0025] (1) The source of the rice curved ball standard sample: rice curved balls stored at room temperature for 3 months.
[0026] (2) Culture medium preparation
[0027] A: The medium used for separation is Wakimoto Tetsu’s medium: 300g potato, 5g peptone, 15g sucrose, 0.5g Ca(NO3)2 4H2O, 2.0g Na2HPO4 12H:0, 16g agar, add water to 1000mL . The culture medium was sterilized by high-pressure damp heat at 121°C for 20 minutes, and when the culture medium was cooled to 45°C-50°C, add chloramphenicol (50 μg / ml) to inhibit bacterial growth, mix well, and pour it into a petri dish, and the medium was solidified for later use use.
[0028] B: The culture medium used is PSA solid medium and PS liquid medium. The PSA solid medium is 200g of potatoes, 20g of agar, 20g of sucrose, and dilute to 1000mL with water. PS liquid medium is 200g of potatoes, 20g of sucrose, and dilute to 1000mL with water. All the above culture media were sterilized under high pressure and damp heat at 121°...
Embodiment 2
[0038] Example 2 Comparison of the results of several methods for the isolation of rice smut bacteria
[0039] 1, the effective isolation method of rice false smut bacteria of the present invention, its step is as follows:
[0040] 1) Isolation of rice smut bacteria
[0041] Same as Example 1
[0042] 2) The characteristics of growth and conidia germination of rice false smut and microscopic observation
[0043] Same as Example 1
[0044] 2. Chlamydospore suspension method
[0045] Referring to Zhou Yongli's 1999 method (Zhou Yongli, 1999, A Preliminary Study on the Isolation Technology of Rice Algeria, China Rice Science, 1999.13(3): l86-188), that is, the rice balls were sterilized with 75% ethanol for 30 seconds, and then sterile Wash 3 times with water, then place in an Eppendorf tube and vibrate to prepare a chlamydospore suspension, take 2 to 3 drops and add it to a petri dish, and pour it into a petri dish when the culture medium is cooled to 45°C to 50°C, gently S...
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