Method capable of rapidly evaluating extent of low temperature injury of straw mushroom

Abstract

The invention relates to a method capable of rapidly evaluating an extent of low temperature injury of straw mushroom. The method capable of rapidly evaluating the extent of low temperature injury of the straw mushroom includes: (1) inoculating activated straw mushroom bacterial strains on a sterile personal digital assistant (PDA) culture medium, culturing for three to four days through static culture; transferring hypha to PDY culture solution after the hypha overgrows a plate, culturing for three to four days through shake cultivation; (2) filtering the hypha in the PDY culture solution, conducting low-temperature treatment, respectively taking hypha balls to conduct vibration under temperature of twenty to twenty-five DEG C at each low-temperature treatment time point; determining an initial conductivity E1, sterilizing again, vibrating, determining a final conductivity E2; and evaluating the extent of low temperature injury of straw mushroom according to a calculated result. The method capable of rapidly evaluating the extent of low temperature injury of the straw mushroom is easy and convenient to operate and easy to master, enables the identification of the extent of low temperature injury of the straw mushroom of a whole system to be conducted under a controllable condition, enables determined data to be reliable, is capable of being used in rapid judgment of low temperature resistance among different straw mushroom bacterial strains and is capable of greatly reducing a breeding period of screening low temperature resistant mutant strains.

Description

technical field [0001] The invention belongs to the field of evaluating the degree of low temperature damage to edible fungi, in particular to a method for quickly evaluating the degree of low temperature damage to straw mushrooms. Background technique [0002] Straw mushroom (Volvariella volvacea (Bull ex Fr.) Sing), also known as straw mushroom, orchid mushroom, and Chinese mushroom, belongs to Basidiomycetes, Agaricales, Phyllosporaceae, and Physophthora genus. Straw mushroom is delicious and nutritious, and it is a high-quality mushroom species in edible fungi. According to research reports, fresh straw mushroom contains 92.39% water, 2.66% protein, 2.24% fat, 1.66% reducing sugar, 0.95% invert sugar, and 0.91% ash; there are 18 kinds of amino acids in the straw mushroom protein, including 8 that cannot be synthesized by the human body. an essential amino acid. In addition to edible value, straw mushrooms also have the effects of improving human immunity, inhibiting th...

AI Technical Summary

Problems solved by technology

Although low temperature-induced genes have been reported at the molecular level, there is still a lack of a physiological and biochemical method for quickly identifying the degree of low-temperature injury of V.

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