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Preparation method for low-phytic acid calcium-tolerant soybean protein

A technology of soybean protein and phytic acid, which is applied in the field of food processing, can solve problems affecting food quality, affecting protein calcium resistance, protein functional properties, and affecting protein extraction rate, so as to weaken electrostatic interaction, increase protein retention rate, and reduce Effect of membrane fouling phenomenon

Active Publication Date: 2013-04-24
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most calcium supplement products currently on the market are derived from milk. However, due to the sensitivity of soybean protein to metal ions such as calcium, adding soybean protein to foods rich in minerals (especially alkali metal elements) tends to aggregate and form Flocculation and precipitation affect the quality of food, thus limiting the application of soybean protein in soy milk, special functional products such as medical clinical nutrition, liquid food, etc.
[0003] At present, phytase treatment is used alone in the industry to remove phytic acid, but the removed phytic acid and other small molecules cannot be completely dialyzed and exist in the solution in free form, which still affects the calcium resistance of the protein and the functional properties of the protein itself. , the effect is not very significant
When the ultrafiltration membrane molecular interception is large, the protein retention rate is greatly reduced, which affects the protein extraction rate. When the ultrafiltration membrane molecular interception is low, the membrane fouling is very serious, causing difficulties for industrial production.
From the perspective of calcium, using molecular calcium (such as calcium carbonate, calcium phosphate, etc.) or embedding calcium can reduce the contact between calcium and protein to a certain extent to avoid precipitation, but these methods are not conducive to calcium absorption.

Method used

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  • Preparation method for low-phytic acid calcium-tolerant soybean protein

Examples

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Effect test

Embodiment 1

[0025] A preparation method of low phytic acid and calcium-tolerant soybean protein: mix defatted soybean flour (g) with water (ml) in a mass volume ratio of 1:10, fully stir and control the temperature to 50°C, add phytase and adjust the temperature with hydrochloric acid When the pH value reaches 4.5, the amount of phytase is 3000U (relative to each gram of soybean flour) and is treated with phytase for 2 hours under stirring, then the pH is adjusted to 8.0 with sodium hydroxide, and after stirring, it is homogenized in a colloid mill for 10 minutes to obtain The defatted soy flour slurry; the defatted soy flour slurry is subjected to jet cooking heat treatment, the treatment temperature is 140 ° C, and the time is 90 s; the jet cooking slurry is adjusted to pH 7.5 with sodium hydroxide, and the obtained heat treated slurry is cooled to 25 ° C. Centrifuge for 30min at a speed of 3000rpm; take the centrifuged supernatant, adjust the pH of the supernatant to 4.5 with hydrochlor...

Embodiment 2

[0027] A preparation method of low phytic acid and calcium-resistant soybean protein: mix defatted soybean powder (g) with water (ml) in a mass-volume ratio of 1:15, fully stir and control the temperature to 50°C, add phytase and adjust the temperature with hydrochloric acid The pH value is 5.5, and the amount of phytase is 2000U (relative to each gram of soybean flour) and is treated with phytase for 2 hours under stirring, and the pH is adjusted to 8.5 with sodium hydroxide, and after stirring, it is homogenized in a colloid mill for 30 minutes, and the obtained Defatted soy flour slurry; heat-treat the defatted soy flour slurry by jet cooking at a temperature of 120°C for 10s; adjust the pH value of the jet-cooked slurry to 8.5 with sodium hydroxide, cool the resulting heat-treated slurry to 25°C, and centrifuge Treat for 30 minutes with a centrifugation speed of 3000rpm; take the centrifuged supernatant, adjust the pH of the supernatant to 4 with hydrochloric acid, re-disso...

Embodiment 3

[0029] A preparation method of low phytic acid and calcium-resistant soybean protein: mix defatted soybean powder (g) with water (ml) in a mass-volume ratio of 1:15, fully stir and control the temperature to 50°C, add phytase and adjust the temperature with hydrochloric acid The pH value is 4.5, and the amount of phytase is 1000U (relative to each gram of soybean flour) and is treated with phytase for 2 hours under stirring, and the pH is adjusted to 7.5 with sodium hydroxide, and after stirring, it is homogenized in a colloid mill for 30 minutes to obtain Defatted soy flour slurry; heat-treat the defatted soy flour slurry by jet cooking at a temperature of 150°C for 100s; adjust the pH of the jet-cooked slurry to 7.5 with sodium hydroxide, cool the resulting heat-treated slurry to 25°C, and centrifuge Treat for 30 minutes, centrifuge at a speed of 3000rpm; take the centrifuged supernatant, adjust the pH of the supernatant to 4.5 with hydrochloric acid, re-dissolve the precipit...

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Abstract

The invention discloses a preparation method for low-phytic acid calcium-tolerant soybean protein. The method comprises the following steps of dissolving low-temperature defatted soybean flour into water, and processing low-temperature defatted soybean flour milk for more than 2 h by phytase to obtain soybean flour milk which is preliminarily processed; performing jet cooking on the soybean flour milk, regulating the pH value to be between 7.5 and 8.5, centrifugally separating, regulating the pH value of supernatant fluid to be between 4.0 and 4.5, dissolving precipitation again with water after centrifuging, and regulating the pH value to be between 6.5 and 7.5; filtering obtained protein solution by an ultrafiltration membrane of which the molecular weight cutoff is 80 kDa, performing spray drying or freeze drying on concentrated solution, and thus obtaining the low-phytic acid calcium-tolerant soybean protein powder. A production process of the low-phytic acid calcium-tolerant soybean protein is low in production cost, the content of the phytic acid reaches 0.12 percent, and the calcium tolerance amount of the protein is 1.0 percent of the weight of the protein. The low-phytic acid calcium-tolerant soybean protein served as a substitute of sodium caseinate can be applied to child and adolescent nourishments, calcium supplement food and medical and clinical nourishments.

Description

technical field [0001] The invention belongs to the technical field of food processing, and relates to a preparation method of functional soybean protein with low phytic acid content and good calcium tolerance and an application example of the soybean protein. Background technique [0002] Soy protein is a high-quality vegetable protein comparable to animal protein, and it is a healthy food raw material with a wide range of sources. At present, domestic soybean protein deep-processing products mainly include soybean protein isolate (SPI) and soybean protein concentrate (SPC). Soybean contains high phytate, the content is as high as 17.9mg / g (dry weight). When soy protein isolate is used in the production of dairy products, the content of phytic acid is too high, which will not only directly affect the solubility characteristics, but also reduce the The calcium content in dairy products also largely affects the body's absorption of nutritional elements such as Ca, Mg, Zn, Cu...

Claims

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Application Information

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IPC IPC(8): A23J1/14
Inventor 杨晓泉张元元尹寿伟王金梅郭健
Owner SOUTH CHINA UNIV OF TECH
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