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Synthesis method and application of fluorescence DNA-polyphenylene acetylene hydrogel

A polyphenylene acetylene and synthesis method technology, applied in the research field of DNA hybrid hydrogel, can solve the problems of cracking, complex preparation methods, cell membrane aggregation, etc., and achieve mild conditions, controllability, and simple synthesis method Effect

Inactive Publication Date: 2013-04-24
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] There are many literature reports on many electrolytes that can undergo conjugated polymerization with them. For example, water-soluble conjugated oligomeric electrolytes have been proposed. Among them, some water-soluble conjugated oligomeric electrolytes have bactericidal effects and good water solubility. The cell membrane has a good affinity, which leads to cell membrane aggregation, cleavage and even death
However, its preparation method is complicated, and its sterilization process is uncontrollable when it is used for sterilization, so it is necessary to design a controllable release bactericide system

Method used

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  • Synthesis method and application of fluorescence DNA-polyphenylene acetylene hydrogel
  • Synthesis method and application of fluorescence DNA-polyphenylene acetylene hydrogel
  • Synthesis method and application of fluorescence DNA-polyphenylene acetylene hydrogel

Examples

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Effect test

Embodiment 1

[0024] Taking 1 g of the raw material oligophenylene acetylene as an example, the method for synthesizing fluorescent DNA-oligophenylene acetylene hydrogel is: 35 g of molecular weight 1 × 10 6 ~1×10 7 DNA (Sigma Company) was dissolved in hydroxyethylpiperazine ethylsulfonic acid (Sigma Company) solution with a concentration of 0.01mol / L and a pH of 7 until completely dissolved to form a DNA solution, to which 25g of ethylene glycol diol was added Glycidyl ether (Bailingwei Technology Co., Ltd.), and then dissolve 1g of oligophenylene acetylene in water until it is completely dissolved to form an oligophenylene acetylene solution. Add the oligophenylene acetylene solution to the solution of the above DNA, and oligomerize The mass ratio of phenylene acetylene to DNA and ethylene glycol diglycidyl ether was 1:35:25, and the DNA-oligophenylene acetylene hydrogel was obtained by dialysis in ultrapure water for 20 hours.

[0025] For the preparation method of the above-mentioned o...

Embodiment 2

[0027] Taking 1 g of raw material oligophenylene acetylene as an example, the method for synthesizing fluorescent DNA-oligophenylene acetylene hydrogel is: 40 g of molecular weight is 1 × 10 6 ~1×10 7 The DNA (Sigma Company) was dissolved in the hydroxyethylpiperazine ethylsulfonic acid (Sigma Company) solution with a concentration of 0.01mol / L and a pH of 7 until completely dissolved to form a DNA solution, to which 28g of ethylene glycol diol was added Glycidyl ether (Bailingwei Technology Co., Ltd.), and then dissolve 1g of oligophenylene acetylene in water until it is completely dissolved to form an oligophenylene acetylene solution. Add the oligophenylene acetylene solution to the solution of the above DNA, and oligomerize The mass ratio of phenylene acetylene to DNA and ethylene glycol diglycidyl ether was 1:40:28, and the DNA-oligophenylene acetylene hydrogel was obtained by dialysis in ultrapure water for 20 hours.

Embodiment 3

[0029] Taking 1 g of raw material oligophenylene acetylene as an example, the method for synthesizing fluorescent DNA-oligophenylene acetylene hydrogel is: 30 g of molecular weight 1 × 10 6 ~1×10 7 The DNA (Sigma Company) was dissolved in the solution of hydroxyethylpiperazine ethylsulfonic acid (Sigma Company) with a concentration of 0.01mol / L and a pH of 7 until it was completely dissolved to form a DNA solution, and 20g of ethylene glycol diol was added thereto. Glycidyl ether (Bailingwei Technology Co., Ltd.), and then dissolve 1g of oligophenylene acetylene in water until it is completely dissolved to form an oligophenylene acetylene solution. Add the oligophenylene acetylene solution to the solution of the above DNA, and oligomerize The mass ratio of phenylene acetylene to DNA and ethylene glycol diglycidyl ether was 1:30:20, and the DNA-oligophenylene acetylene hydrogel was obtained by dialysis in ultrapure water for 20 hours.

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Abstract

The invention discloses a synthesis method for synthesizing a fluorescence DNA-polyphenylene acetylene hydrogel, and the method adopts DNA as a hydrogen netlike frame to synthesize a novel DNA hybrid hydrogel together with polyphenylene acetylene by electrostatic interaction. The synthesis method is simple and mild in condition; the hydrogel can effectively coat the polyphenylene acetylene in the netlike frame of the DNA so as to prevent the polyphenylene acetylene from having contact with bacteria; the hydrogel can be applied to sterilization regulation and control; when the hydrogel is added with deoxyribonuclease, the netlike frame of the DNA is damaged to release polyphenylene acetylene, the dissociative polyphenylene acetylene can approach to the bacteria or enter the bacteria to generate singlet oxygen or active oxygen substance by the irradiation of white light, so that the bacteria are killed; and the deoxyribonuclease can be utilized to realize controllable sterilization.

Description

technical field [0001] The invention belongs to the technical field of research on DNA hybrid hydrogels, and in particular relates to a synthesis method of fluorescent DNA-oligophenylene acetylene hydrogels and its new application in sterilization regulation. Background technique [0002] The gel state is similar to a solid state, and its molecules form a three-dimensional network structure through some non-covalent forces, and the solvent molecules are fixed in it. [0003] In recent years, deoxyribonucleic acid (DNA) hydrogels have broad application prospects in drug release, tissue engineering, imaging, medical equipment, gene therapy, and biosensing. Hydrogels have a three-dimensional network structure and can absorb a large amount of The water swells, and after swelling, it continues to maintain its original structure without being dissolved. Hydrogels are similar to living tissue materials and exhibit good biocompatibility when in contact with blood, body fluids and h...

Claims

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Application Information

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IPC IPC(8): A01N31/16A01N25/26A01P1/00
Inventor 唐艳丽曹阿丽刘越
Owner SHAANXI NORMAL UNIV
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