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Production method and application of immune globulin G

A technology of immunoglobulin and products, applied in the direction of immunoglobulin, immunoglobulin from serum, application, etc., can solve the problems of not being suitable for large-scale industrial production, small production scale, and cumbersome production process

Inactive Publication Date: 2013-04-17
SHANGHAI GENON BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the production of immunoglobulin products at home and abroad is usually through laboratory trial methods such as ammonium sulfate precipitation and ethanol precipitation. The production process is cumbersome and the production scale is small, which is not suitable for large-scale industrial production.

Method used

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  • Production method and application of immune globulin G
  • Production method and application of immune globulin G
  • Production method and application of immune globulin G

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0135] small amount of immunoglobulin G

[0136] 1. Whole blood anticoagulation

[0137] The anticoagulant potassium oxalate, sodium chloride, and sodium citrate were mixed according to the ratio of 3:2:5 to configure an anticoagulant solution with a concentration of 10%, with a total volume of 1 kg, and added to 9 kg of whole blood to obtain Anticoagulated whole blood;

[0138] 2. Centrifuge

[0139] Centrifuge the anticoagulated whole blood through a tube centrifuge (16,000 rpm) to obtain 5.3 kg of light liquid plasma and 4.7 kg of heavy liquid blood cells;

[0140] 3. Dilution

[0141] Add 40 kg of pure water to the centrifuged light plasma to obtain the diluted plasma;

[0142] 4. Precipitation reaction

[0143] Addition of FeCl to diluent plasma 3 , so that FeCl in the liquid 3 The concentration is 5mmol / L, the pH of the material is adjusted to 3.5, the temperature is 45°C, and the precipitation reaction is carried out for 1 hour to obtain the reaction solution;

...

Embodiment 2

[0158] mass production of immunoglobulin G

[0159] 1. Whole blood anticoagulation

[0160] Take 1 ton of pig blood, mix the anticoagulants potassium oxalate, sodium chloride, and sodium citrate in a ratio of 3:2:5, take 8 kilograms to prepare an anticoagulant solution with a concentration of 10%, and add it while stirring. Obtain anticoagulated whole blood;

[0161] 2. Centrifuge

[0162] Centrifuge the anticoagulated whole blood through a tube centrifuge (16,000 rpm) to obtain 600 kg of light liquid plasma and 480 kg of heavy liquid blood cells;

[0163] 3. Dilution

[0164] Add 4000 kg of pure water to 600 kg of plasma obtained by centrifugation to obtain the diluted plasma;

[0165] 4. Precipitation reaction

[0166] Addition of FeCl to diluent plasma 3 , so that FeCl in the liquid 3 The concentration is 5mmol / L, the pH of the material is adjusted to 5, the temperature is 40°C, and the precipitation reaction is carried out for 2 hours to obtain the reaction solution...

Embodiment 3

[0181] Preparation of Feed Containing Immunoglobulin G and Its Effect

[0182] Feed 1

[0183] There are 99 parts of commercially available suckling pig feed and 1 part of immunoglobulin G.

[0184] Feed 2

[0185] 100 parts of commercially available suckling pig feed.

[0186] The above two feeds were fed to piglets, and the results showed that the diarrhea rate of piglets fed with immunoglobulin products could be reduced by more than 50%, and the blood immunoglobulin content increased by more than 40%. It shows that the immunoglobulin G prepared by the method of the invention has significant curative effect on intestinal diseases of young animals.

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Abstract

The invention relates to a production method and an application of an immune globulin G. Specifically, the production method comprises steps of: carrying out whole blood anticoagulating, centrifugal separation, diluting, FeCl3 precipitation reaction, clarifying, centrifugal separation, nano filtration and desalination, embedding, spray-drying, etc. The production method disclosed by the invention not only solves the problems of resource waste, environmental pollution and the like of animal byproduct pig blood, but also greatly improves the utilization value of the pig blood through continuous mass production processing process; the prepared immune globulin G, through an enzyme-linked immuno sorbent assay (ELISA) activity identification, is greater than 90% in activity, and has a special treating effect on intestinal diseases of small animals; and the immune globulin G can be used as an immuno potentiator for a weak animal group and as normal nutrition supplementary for the body.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for producing immunoglobulin G and its application. Background technique [0002] Our country is very rich in animal blood resources. About 2.3 million tons of animal blood are produced every year, but most of them are not properly processed and used. Only a small part is processed into mid-range products such as plasma protein powder and blood globulin powder. Therefore, animal blood products The added value is very low, causing great waste. [0003] Immunoglobulin G is ubiquitously present in blood, interstitial fluid, and exocrine fluid of mammals and humans, and is widely used in the biomedical field. Animal blood is rich in immunoglobulin, and its content is generally 25-35%. Immunoglobulin is an immune component that protects the body against external infections. It is the most durable and important antibody in the primary immune response. It has antibacterial, antit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/00C07K16/06A23K1/16A23L1/305A61K39/395A61P1/12A61P1/00
Inventor 成国祥江国永于伟
Owner SHANGHAI GENON BIOLOGICAL PROD
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