Production method and application of immune globulin G
A technology of immunoglobulin and products, applied in the direction of immunoglobulin, immunoglobulin from serum, application, etc., can solve the problems of not being suitable for large-scale industrial production, small production scale, and cumbersome production process
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Embodiment 1
[0135] small amount of immunoglobulin G
[0136] 1. Whole blood anticoagulation
[0137] The anticoagulant potassium oxalate, sodium chloride, and sodium citrate were mixed according to the ratio of 3:2:5 to configure an anticoagulant solution with a concentration of 10%, with a total volume of 1 kg, and added to 9 kg of whole blood to obtain Anticoagulated whole blood;
[0138] 2. Centrifuge
[0139] Centrifuge the anticoagulated whole blood through a tube centrifuge (16,000 rpm) to obtain 5.3 kg of light liquid plasma and 4.7 kg of heavy liquid blood cells;
[0140] 3. Dilution
[0141] Add 40 kg of pure water to the centrifuged light plasma to obtain the diluted plasma;
[0142] 4. Precipitation reaction
[0143] Addition of FeCl to diluent plasma 3 , so that FeCl in the liquid 3 The concentration is 5mmol / L, the pH of the material is adjusted to 3.5, the temperature is 45°C, and the precipitation reaction is carried out for 1 hour to obtain the reaction solution;
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Embodiment 2
[0158] mass production of immunoglobulin G
[0159] 1. Whole blood anticoagulation
[0160] Take 1 ton of pig blood, mix the anticoagulants potassium oxalate, sodium chloride, and sodium citrate in a ratio of 3:2:5, take 8 kilograms to prepare an anticoagulant solution with a concentration of 10%, and add it while stirring. Obtain anticoagulated whole blood;
[0161] 2. Centrifuge
[0162] Centrifuge the anticoagulated whole blood through a tube centrifuge (16,000 rpm) to obtain 600 kg of light liquid plasma and 480 kg of heavy liquid blood cells;
[0163] 3. Dilution
[0164] Add 4000 kg of pure water to 600 kg of plasma obtained by centrifugation to obtain the diluted plasma;
[0165] 4. Precipitation reaction
[0166] Addition of FeCl to diluent plasma 3 , so that FeCl in the liquid 3 The concentration is 5mmol / L, the pH of the material is adjusted to 5, the temperature is 40°C, and the precipitation reaction is carried out for 2 hours to obtain the reaction solution...
Embodiment 3
[0181] Preparation of Feed Containing Immunoglobulin G and Its Effect
[0182] Feed 1
[0183] There are 99 parts of commercially available suckling pig feed and 1 part of immunoglobulin G.
[0184] Feed 2
[0185] 100 parts of commercially available suckling pig feed.
[0186] The above two feeds were fed to piglets, and the results showed that the diarrhea rate of piglets fed with immunoglobulin products could be reduced by more than 50%, and the blood immunoglobulin content increased by more than 40%. It shows that the immunoglobulin G prepared by the method of the invention has significant curative effect on intestinal diseases of young animals.
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