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Preparation method for tubular bacterial cellulose

A bacterial cellulose, tubular technology, applied in the field of tubular bacterial cellulose preparation, can solve problems such as poor suture performance, intima hyperplasia, and insufficient compliance, and achieve the effects of increasing production rate, dense and smooth outer wall, and shortening the production cycle

Inactive Publication Date: 2013-04-03
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the tubular bacterial cellulose prepared only by a single-layer oxygen-permeable material has an asymmetric structure with a dense inner cavity and a loose outer wall.
The loose outer wall makes the material less seamable
It is easy to form intimal hyperplasia at the suture site due to insufficient compliance between the material and the surrounding tissue, which in turn leads to the occurrence of thrombus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] 1) Preparation of fermentation medium;

[0054] Components of the fermentation medium, in mass percent, in wt%: glucose, fructose, sucrose or mannitol 2, peptone 0.05, yeast extract 0.05, citric acid 0.01, disodium hydrogen phosphate 0.02, potassium dihydrogen phosphate 0.01, and amount of water;

[0055] The pH of the fermentation broth is 4.0;

[0056] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;

[0057] 2) Bacteria expansion;

[0058] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of strain cells is 2×10 5 pieces / ml.

[0059] 3) Static cultivation;

[0060] After the expanded bacterial liquid is evenly mixed with the prepared fermentation culture liquid, it is poured into the area between the outer wall of the inner tube and the inner wall of the outer...

Embodiment 2

[0070] 1) Preparation of fermentation medium;

[0071] Components of the fermentation medium, in mass percent, in wt%: 5 glucose, fructose, sucrose or mannitol, 0.5 peptone, 0.5 yeast extract, 0.1 citric acid, 0.2 disodium hydrogen phosphate, 0.1 potassium dihydrogen phosphate, and amount of water;

[0072] The pH of the fermentation broth is 6.0;

[0073] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;

[0074] 2) Bacteria expansion;

[0075] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of strain cells is 2×10 7 pieces / ml.

[0076] 3) Static cultivation;

[0077] After the expanded bacterial liquid is evenly mixed with the prepared fermentation culture liquid, it is poured into the area between the outer wall of the inner tube of the hollow oxygen-permeable mold ...

Embodiment 3

[0087] 1) Preparation of fermentation medium;

[0088] Components of the fermentation medium, in mass percent, in wt%: 3 glucose, fructose, sucrose or mannitol, 0.3 peptone, 0.3 yeast extract, 0.05 citric acid, 0.1 disodium hydrogen phosphate, 0.05 potassium dihydrogen phosphate, and amount of water;

[0089] The pH of the fermentation broth is 5.0;

[0090] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;

[0091] 2) Bacteria expansion;

[0092] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of strain cells is 2×106 pieces / ml.

[0093] 3) Static cultivation;

[0094] After the cultured bacteria liquid is evenly mixed with the prepared fermentation culture liquid, it is poured into the area between the outer wall of the inner tube of the hollow oxygen-permeable mold a...

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Abstract

The invention relates to a preparation method for tubular bacterial cellulose, which adopts double layers of oxygen permeation materials to form a hollow mould by nesting for culturing bacterial cellulose. Based on the disclosed technology, the pressure of internal pressure gas of inner tube of a hollow oxygen permeation mould, external gas of an outer tube of the hollow oxygen permeation mould, and oxygen volume concentration are precisely adjusted, so that the inner tube and the outer tube of the hollow oxygen permeation mould can generate periodic alternating changing of shrinkage and expansion along the radial direction. The radial deformation amplitude is small and does not exceed 5%, and so the cultivation process is a quasi-static process. Based on the prior art, the preparation method provided by the invention doubles the effective region in the bacterial cellulose, shortens the cultivation period of the tubular bacterial cellulose, enables the outer wall of the tubular bacterial cellulose to be more compact, improves the sewing performance of the material and reduces the probability that the tubular bacterial cellulose generates intimal hyperplasia and thrombus at the sewn part. Besides, the preparation method has a short preparation period, a green and environment-friendly preparation process, simplicity and quickness, and low preparation cost.

Description

technical field [0001] The invention relates to a preparation method of tubular bacterial cellulose. Background technique [0002] Bacterial cellulose refers to the general term for cellulose synthesized by certain microorganisms in the genus Acetobacter, Agrobacterium, Rhizobium and Sarcina under different conditions. Acetobacter xylinum in the genus Acetobacter is widely used as a template microorganism for basic and applied research on bacterial cellulose because of its highest cellulose production efficiency. [0003] The preparation methods of bacterial cellulose are mainly static culture and dynamic culture. Due to the oxygen-dependent survival properties of the Gram-negative primary cell body, bacterial cellulose is formed at the junction of the sugar source and the aerobic region. The bacterial cellulose material obtained by static culture can form regular geometric shapes such as film and tube according to the different shapes of culture containers and application...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/01C12R1/02C12R1/38C12R1/41
Inventor 陈仕艳杨敬轩郑羿李喆王利群王华平
Owner DONGHUA UNIV
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