Actinomycete capable of inhibiting phytophthoramelonis and screening method thereof
A cucumber Phytophthora, inhibition technology, applied in the field of new strains, can solve the problems of human health hazards, large environmental impact of chemical agents, easy to produce drug resistance, etc., and achieve good application prospects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0023] The screening method of the actinomycetes that the present invention has inhibitory effect to cucumber Phytophthora is as follows:
[0024] 1. Collection of soil samples
[0025] Four soil samples were randomly collected from near the Jinbian River in Zhangjiajie National Forest Park, Hunan Province. When collecting soil samples, first remove the topsoil, take about 200g of soil samples at a depth of 5-20cm, pack and mark them, and bring them back to the laboratory.
[0026] 2. Isolation of actinomycetes
[0027] Separation by dilution. Weigh 10 grams of the soil sample, put it into a conical flask equipped with small glass beads and 90 ml of sterile water, shake it for 20 minutes to dilute it. After standing for 30 seconds, dilute 10 times sequentially to prepare 10 -4 、10 -5 、10 -6 Dilution solution, draw 0.1ml of the dilution solution and add dropwise to Gao’s No. 1 medium (20 grams of soluble starch, 20 grams of agar, K 2 HPO 4 0.5g, NaCl 0.5g, KNO 3 1 g...
Embodiment 2
[0044] On February 10, 2012, cucumber seedlings were cultivated, and the variety was Shuyan Baiyu. Soak cucumber seeds in warm water at 50°C for 10 minutes, then overnight at 26°C. On February 11, the seeds were wrapped in gauze and kept at 26°C to accelerate germination. On February 12th, prepare seedling trays, sow seeds, and put them into a light incubator at 27°C for 12h during the day and 16°C for 12h at night, keeping RH80%. On February 29, the cucumber seedlings were inoculated with sporangia of Phytophthora cucumber when two true leaves appeared, and the concentration of sporangia was 5×10 3 zoospores / ml. Before inoculation, water the seedling tray thoroughly, and use the root irrigation inoculation method to take 2ml of inoculation suspension and add it around the roots of the plants. The inoculated cucumber seedlings were divided into 2 groups. One group was perfused with 2ml diluted 20×Gaoshi No. 1 culture solution in the roots, and the other group was perfused ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com