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Biodegradable and medical tricalcium phosphate/gamma-polyglutamic acid composite and preparation method thereof

A technology of tricalcium phosphate and polyglutamic acid, applied in the field of biomedicine, can solve the problems of difficult control of degradation rate, limited depth of bone ingrowth, and long time, etc., and achieve the effect of uniform distribution of tricalcium phosphate and appropriate pore size

Active Publication Date: 2013-04-03
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when tricalcium phosphate is used alone as a bone repair material, there are various defects, which limit its clinical application.
The main disadvantages of tricalcium phosphate are: (1) It has poor toughness, high brittleness, low flexural strength, and cannot bear weight, so it can only be used for non-load-bearing bone defects; (2) Lack of inductive activity, the material can only It can grow bone tissue by osteoconduction, the amount of bone growth is small, it takes a long time, and the depth of bone ingrowth is limited, so it is not suitable for repairing long bone defects; (3) The degradation rate is difficult to control and cannot match the growth rate of new bone

Method used

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  • Biodegradable and medical tricalcium phosphate/gamma-polyglutamic acid composite and preparation method thereof
  • Biodegradable and medical tricalcium phosphate/gamma-polyglutamic acid composite and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Dissolve 1 g of γ-polyglutamic acid in 50 mL of deionized water, and stir until a transparent viscous emulsion is formed to obtain a γ-polyglutamic acid solution, which is set aside. Add 5g of β-tricalcium phosphate into 10ml of 0.1mol / L dilute hydrochloric acid, stir for 0.5h until completely dissolved, then treat it with ultrasonic waves (ultrasonic frequency: 40KHZ, 10min), and then use sodium hydroxide solution to bring the pH value to 4.0 , and set the volume to 50mL to obtain a β-tricalcium phosphate solution. Add gamma-polyglutamic acid solution into the treated beta-tricalcium phosphate solution, stir and mix evenly to obtain gamma-polyglutamic acid / beta-tricalcium phosphate solution. In the γ-polyglutamic acid / β-tricalcium phosphate solution, add 10ml of an aqueous solution containing 0.05g of allyl glycidyl ether dropwise under stirring, and stir the mixture at room temperature for 1 hour, and a white colloidal suspended substance appears in the solution , st...

Embodiment 2

[0031]Dissolve 5 g of γ-polyglutamic acid in 50 mL of deionized water, and stir until a transparent viscous emulsion is formed to obtain a γ-polyglutamic acid solution, which is set aside. Add 1g of β-tricalcium phosphate into 10ml of 1.0mol / L dilute hydrochloric acid, stir for 1h until completely dissolved, then treat it with ultrasonic waves (ultrasonic frequency: 40KHZ, 10min), adjust the pH value to 5.0 with ammonia solution, and dilute to 40mL , to obtain β-tricalcium phosphate solution. Add gamma-polyglutamic acid solution into the treated beta-tricalcium phosphate solution, stir and mix evenly to obtain gamma-polyglutamic acid / beta-tricalcium phosphate solution. In the γ-polyglutamic acid / β-tricalcium phosphate solution, add dropwise 10ml of an aqueous solution containing 0.2g of glycerol triglycidyl ether, and stir the mixture at room temperature for 1 hour. A white colloidal suspended substance appears in the solution. Stop stirring. After all the colloidal substance...

Embodiment 3

[0034] Dissolve 2.5g of γ-polyglutamic acid in 50mL of deionized water, and stir until a transparent viscous emulsion is formed to obtain a γ-polyglutamic acid solution, which is set aside. Add 10g of β-tricalcium phosphate to 20mL of 1.0mol / L dilute hydrochloric acid, stir for 0.5h until completely dissolved, then treat it with ultrasonic waves (ultrasonic frequency: 40KHZ, 10min), then adjust the pH value to 6.0 with sodium hydroxide, and set Make up to 40mL to obtain β-tricalcium phosphate solution. Add gamma-polyglutamic acid solution into the treated beta-tricalcium phosphate solution, stir and mix evenly to obtain gamma-polyglutamic acid / beta-tricalcium phosphate solution. In the γ-polyglutamic acid / β-tricalcium phosphate solution, add dropwise 10ml aqueous solution containing 1.0g polyethylene oxide sorbitol polyglycidyl ether under stirring, and stir the mixture at room temperature for 1h, and the solution appears White colloidal suspended substance, stop stirring, af...

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Abstract

The invention discloses a biodegradable and medical tricalcium phosphate / gamma-polyglutamic acid composite and a preparation method thereof. According to the invention, an in situ complexing method is adopted for preparing the biodegradable and medical tricalcium phosphate / gamma-polyglutamic acid composite, the composite is formed by complexing tricalcium phosphate and gamma-polyglutamic acid by virtue of intermolecular group carboxylate radicals, tricalcium phosphate powder is deposited on a gamma-polyglutamic acid substrate, mechanical strength of the composite is enhanced under the action of a crosslinking agent, toughness of tricalcium phosphate is improved, and bone bonding ability and degradability of the tricalcium phosphate are improved, so that repair of bone defect can be promoted. Pore size of the composite is 60-280Mum, porosity range is 64-85%, weight loss ratio is 8.60-39.57% after the composite is soaked in 0.9% normal saline and stands still for three months at constant temperature of 37 DEG C, the composite has the characteristics that the tricalcium phosphate is uniformly distributed, the tricalcium phosphate and the gamma-polyglutamic acid are tightly combined and degradation rate is continuous and adjustable, the pore size of the composite is appropriate, and the composite can be taken as bone repair material to be applied to the biomedical fields such as bone tissue engineering.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a biodegradable medical tricalcium phosphate / γ-polyglutamic acid composite material and a preparation method thereof. Background technique [0002] Tricalcium phosphate (TCP) is an artificial bone graft material developed in recent years. It is mainly composed of calcium and phosphorus ions, and its chemical formula is Ca 3 (PO 4 ) 2 , similar to the inorganic components in bone, including high-temperature α-phase and low-temperature β-phase. TCP has been used as a bone repair material for more than 30 years. However, when tricalcium phosphate is used alone as a bone repair material, there are various defects, which limit its clinical application. The main disadvantages of tricalcium phosphate are: (1) It has poor toughness, high brittleness, low flexural strength, and cannot bear weight, so it can only be used for non-load-bearing bone defects; (2) Lack of inductive ac...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/12A61L27/56
Inventor 疏秀林施庆珊欧阳友生陈仪本
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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