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Method for dilution crystallizationelution and crystallization of cytidine 5'-disodium phosphate

A technology of disodium phosphate and dissolving crystallization, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problem of inconvenient storage, transportation and use, poor particle size distribution and crystal habit quality, and amorphous products containing problems such as low water volume, to achieve the effect of good gloss, uniform particle size and short operation time

Inactive Publication Date: 2013-03-06
NANJING UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, domestic production of cytidine 5'-disodium phosphate is basically amorphous, and the particle size distribution and crystal habit quality of crystalline products are poor, such as poor fluidity and small specific gravity, and amorphous products have low water content and are unstable. Subsequent storage, transportation and use bring inconvenience
At present, there is little research on the crystallization process of cytidine 5'-phosphate disodium salt in China, and there is no relevant literature report

Method used

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  • Method for dilution crystallizationelution and crystallization of cytidine 5'-disodium phosphate
  • Method for dilution crystallizationelution and crystallization of cytidine 5'-disodium phosphate
  • Method for dilution crystallizationelution and crystallization of cytidine 5'-disodium phosphate

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Effect test

Embodiment 1

[0026] Add 10 L of cytidine 5'-phosphate disodium aqueous solution with a concentration of 10 g / L, pH 7.9, and slowly add 29 L of anti-solvent anhydrous ethanol under the conditions of controlling the water bath at 20 °C and stirring at 150 rpm. After the crystallization was complete, the suspension was filtered with suction, washed with a small amount of ethanol to obtain white crystals, and placed in an oven at 45°C for vacuum drying. The crystallization yield was 95.1%, and the purity was 99.1%.

Embodiment 2

[0028] Add 6 L of cytidine 5'-phosphate disodium aqueous solution with a concentration of 100 g / L, pH 7.0, and slowly add 14 L of anti-solvent methanol under the conditions of controlling the water bath at 10 ° C and a stirring rate of 250 rpm. After the crystallization was complete, the suspension was filtered with suction, washed with a small amount of ethanol to obtain white crystals, and placed in an oven at 45°C for vacuum drying. The crystallization yield was 96.2%, and the purity was 99.3%.

Embodiment 3

[0030] Add 2L of 180g / L cytidine 5'-disodium phosphate aqueous solution, pH 8.0, in a water bath controlled at 30°C and a stirring rate of 200rpm, and slowly add 4.4L of anti-solvent anhydrous ethanol. After the crystallization was complete, the suspension was filtered with suction, washed with a small amount of ethanol to obtain white crystals, and placed in an oven at 45°C for vacuum drying. The crystallization yield was 95.7%, and the purity was 99.5%.

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Abstract

The invention discloses a method for dilution crystallizationelution and crystallization of cytidine 5'-disodium phosphate. An organic solvent of 0.5-3.0 time(s) of aqueous solution of cytidine 5'-disodium phosphate is added as an anti-solvent into the aqueous solution of cytidine 5'-disodium phosphate with the pH value of 7.0-9.0 and the concentration of 10-300g / L, mixing is performed at temperature of 10-40 DEG C and at a controlled speed of 50-230rmp, suction filtration is performed after complete crystallization, the crystal is washed with ethanol, and vacuum drying is performed to obtained the cytidine 5'-disodium phosphate crystal. By adopting the method, the final product quality can be improved significantly, and the yield of the crystalline product can be increased stably. Moreover, the particle size of the crystal can be controlled by changing the mixing speed or temperature for crystallization. The method has the advantages of simpleness in operation and good repeatability and is suitable for industrial production of the cytidine 5'-disodium phosphate.

Description

technical field [0001] The invention belongs to the field of biological product processing, and in particular relates to a method for dissolution and crystallization of cytidine 5'-phosphate disodium. Background technique [0002] Cytidine 5'-Disodium Phosphate (5'Cytidine Monophosphate Disodium Salt,5'-CMPNa 2 ) chemical formula is C 9 h 12 N 3 Na 2 o 8 p. The molecular weight is 367.16. It is colorless to white crystal or white crystalline powder. The chemical structure is as follows. [0003] [0004] Cytidine 5'-phosphate disodium has a special taste and a melting point of 300°C. Soluble in water, stable in aqueous solution. It has low solubility in organic solvents and is almost insoluble in organic solvents such as methanol and ethanol. The maximum absorption spectrum of 0.01mol / L hydrochloric acid solution is 279nm±2nm. [0005] Cytidine 5'-phosphate disodium salt can be used as a flavoring agent MSG together with 5'-guanylate disodium salt as a food addi...

Claims

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Application Information

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IPC IPC(8): C07H19/10C07H1/06
Inventor 应汉杰俞金吴菁岚陈勇陈晓春谢婧婧
Owner NANJING UNIV OF TECH
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