Acetobacter strain and application thereof
The technology of Acetobacter and bacterial strain is applied to Acetobacter and its application field in the production of sea buckthorn fruit vinegar, can solve the problems of acetic acid peroxidation, low acetic acid conversion rate and the like, and achieves fast fermentation speed, high acetic acid conversion rate and sour taste. outstanding effect
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Embodiment 1
[0028] Embodiment 1: Acetobacter ( Acetobacter sp. SJA-1) Isolation, screening and identification of CGMCC No.3347
[0029] 1. Isolation and Screening of Strains
[0030] (1) Strain isolation
[0031] Take 10g of the mixed seabuckthorn vinegar mash sample after natural alcohol fermentation and natural acetic acid fermentation at room temperature, put it into a 500mL conical flask with 100mL basal medium, add 6% absolute ethanol, at 120r / min, 30 ℃ in the electric heating air bath shaker, add 1% absolute ethanol every day, take the fermentation broth and dilute it to 10 -5 , 10 -6 , 10 -7 Concentration, spread on the plate separation medium for 5 days, coat 3 plates for each dilution, use 0.1mL dilution for each plate, incubate upside down at 30°C for 48 hours after coating, pick the larger calcium dissolution circle , rich in colonies, single colonies with growth advantages were purified and cultivated, and then the purified strains were inoculated in the slant preservati...
Embodiment 2
[0044] Embodiment 2: Acetobacter ( Acetobacter sp. SJA-1) Application of CGMCC No.3347 in Seabuckthorn Fruit Vinegar Fermentation
[0045] Acetobacter ( Acetobacter sp .SJA-1) CGMCC No.3347 was inoculated into the seed medium (glucose 1.32%, yeast extract powder 1.27%, acetic acid 1.0%, ethanol 3.0%, K 2 HPO 4 0.18%, pH5.0), at 10% inoculum volume, 100mL / 500mL liquid volume, 150r / min shaker speed, culture at 32°C for 24h; then add sea buckthorn wine with 7% inoculum volume , at an initial seabuckthorn wine alcohol content of 5% (vol, 20°C), initial pH of 4.5, liquid volume of 75mL / 500mL, shaker speed of 90r / min, and fermentation at 30°C for 6 days. Under these conditions, the yield of acetic acid can reach 4.82g / 100mL. The fruit vinegar has a suitable sour taste, strong fragrance, harmonious and unified fruit aroma and vinegar aroma, and has distinct characteristics of sea buckthorn fruit vinegar.
Embodiment 3
[0046] Embodiment 3: Acetobacter ( Acetobacter sp. SJA-1) Application of CGMCC No.3347 in Seabuckthorn Fruit Vinegar Fermentation
[0047] Acetobacter ( Acetobacter sp .SJA-1) CGMCC No.3347 was inoculated into the seed medium (glucose 1.32%, yeast extract powder 1.27%, acetic acid 1.0%, ethanol 3.0%, K 2 HPO 4 0.18%, pH5.0), at 10% inoculum volume, 100mL / 500mL liquid volume, 150r / min shaker speed, culture at 32°C for 24h; then add seabuckthorn wine with 8% inoculation volume , at an initial seabuckthorn wine alcohol content of 6% (vol, 20°C), initial pH of 3.5, liquid volume of 100mL / 500mL, shaker speed of 125r / min, and fermentation at 32°C for 5 days. Under these conditions, the yield of acetic acid can reach 5.67g / 100mL. The fruit vinegar has a prominent sour taste, rich and harmonious aroma, and has a unique flavor of sea buckthorn fruit vinegar.
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