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Method for extraction of anemonin from ranunculus

An extraction technology of pulsatilla, which is applied in the biological field, can solve problems such as difficult industrial production, easy poisoning of operators, and expensive equipment, and achieve the effects of less solvent capacity, short extraction time, and simple operation

Inactive Publication Date: 2013-01-30
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the steam distillation temperature is too high, it is easy to change the pulsatilla, and the chloroform extraction in the sodium chloride aqueous solution is inefficient, and the chloroform is highly toxic, and the operator is easy to be poisoned. Preparation method and application of its composition", this invention also adopts distillation method to prepare pulsatilla
with supercritical CO 2 Although the extraction method is efficient and clean, it is difficult to realize industrial production due to the high cost of equipment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Take fresh ranunculus raw material, select and remove impurities, crush, weigh 1kg, put it into the extraction kettle, seal it, pour in deionized water with pH 5, extract for 30 minutes at a temperature of 150°C and a pressure of 2MPa, and add the obtained extract to D101 macropore Adsorbed in a resin column, eluted impurities with 3 column volumes of 50% ethanol solution, and then eluted active ingredients with 4 column volumes of 95% ethanol. The eluent was concentrated under reduced pressure, refrigerated and crystallized, and the crystals were dried at low temperature to obtain 1.1 g of pulsatilla. The content was determined to be 96.3%.

Embodiment 2

[0019] Take the dried ranunculus raw material, select and remove impurities, crush, weigh 2kg, put it into the extraction kettle, seal it, pour in deionized water with pH 5, extract for 40 minutes at a temperature of 130°C and a pressure of 1.5MPa, and add the obtained extract to HPD100 Adsorbed in a porous resin column, eluted impurities with 3 column volumes of 30% ethanol solution, and then eluted active ingredients with 4 column volumes of 90% ethanol. The eluent was concentrated under reduced pressure, refrigerated and crystallized, and the crystals were dried at low temperature to obtain 1.8g of pulsatilla , Assay 95.1%.

Embodiment 3

[0021] Take fresh ranunculus raw materials, select and remove impurities, crush, weigh 5kg, put it into an extraction kettle, seal it, pour deionized water with pH 5, extract for 40 minutes at a temperature of 120°C and a pressure of 1MPa, and add the obtained extract to AB-8 Adsorbed in a macroporous resin column, 4 column volumes of 30% ethanol solution to elute impurities, and then 5 column volumes of 90% ethanol to elute active ingredients, the eluent was concentrated under reduced pressure, refrigerated and crystallized, and the crystals were dried at low temperature to obtain 4.3g of pulsatilla , Assay 91.8%.

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Abstract

The invention discloses a method for extraction of anemonin from ranunculus. Characterized by simple operation, high efficiency and low pollution, the method consists of the steps of: crushing ranunculus to a size of 20-60 meshes, putting the crushed ranunculus into a subcritical extraction kettle, introducing deionized water with pH of 4-5, conducting dynamic extraction under a temperature of 100-180DEG C and a pressure of 0.3-2MPa for 20-60min so as to obtain an extracted solution; and adding the extracted solution into a macroporous resin column to perform adsorption, carrying out ethanol solution gradient elution, and subjecting the eluent to concentration crystallization, thus obtaining the anemonin. During production, the method provided in the invention can reduce solvent capacity, increase product yield, and is easy to realize industrialization.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for extracting pulsatilla from ranunculus. Background technique [0002] Pulsatilla root is also known as anemone, pulsatilla root. Widely present in medicinal plants of the genus Ranunculus, such as Pulsatilla, Clematis, etc., it has a significant antibacterial effect: the inhibitory concentration for Staphylococcus, Streptococcus, and Bacillus diphtheria is 1:12500, for Mycobacterium tuberculosis 1:50000, and for large intestine Bacillus also has a similar antibacterial effect, which is effective against Gram-negative bacteria. It excites and then paralyzes the central nervous system, has analgesic and analgesic effects, and has strong nail fungal activity. Clinically, it has been used as a treatment for dysentery, a sedative and analgesic. [0003] Pulsatilla is a small molecule compound, molecular formula: C 10 h 8 o 4 , molecular weight 192.16, melting point 157-15...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/10
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
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