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Preparation and application of cellulose nanocrystals (CNCs)-reinforced collagen compound substrate

A nano-microcrystalline and composite matrix technology, which is applied in skin care preparations, cosmetic preparations, dressing preparations, etc., can solve problems such as poor mechanical properties, difficulty in shaping, and inability to support tissue reconstruction, and achieve high production efficiency , Improved mechanical strength, and excellent liquid absorption

Active Publication Date: 2015-04-22
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tissue engineered skin prepared from pure collagen matrix has the advantages of good biocompatibility, easy processing, plasticity, and the ability to promote cell adsorption and proliferation, but it also has poor mechanical properties, is difficult to shape when it contains water, and cannot support tissue reconstruction.

Method used

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  • Preparation and application of cellulose nanocrystals (CNCs)-reinforced collagen compound substrate
  • Preparation and application of cellulose nanocrystals (CNCs)-reinforced collagen compound substrate
  • Preparation and application of cellulose nanocrystals (CNCs)-reinforced collagen compound substrate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039]Add 15g of cotton cellulose to 150mL of 64%w / w concentrated sulfuric acid, in a constant temperature water bath at 45°C, mechanically stir the reaction for 6 hours, add 800mL of deionized water to terminate the reaction, after cooling, ultrasonically (35kHz) disperse for 30min, remove After dialysis with ionized water for 3 days, freeze overnight at -20°C, and then freeze-dry in a lyophilizer for 12 hours to prepare nanocrystalline cellulose (cellulose nanocrystals, CNCs). Use the 0.3% acetic acid solution as the solvent to prepare the CNCs solution and the collagen solution with a mass concentration of 1%; get 0.5 g of the above CNCs solution and drop it into the 9.5 g of the above collagen solution, and mechanically stir for 2 hours to obtain the CNCs solution with a content of 5% w / w CNCs / collagen mixed solution, then pour the mixed solution into a polytetrafluoroethylene mold, put it in an oven at 35°C, and make a 5wt% CNCs-reinforced collagen composite film after the...

Embodiment 2

[0041] Rinse the commercially available bacterial cellulose with clean water several times to remove the culture medium and impurities on the surface of the membrane, then soak the membrane in 0.5mol / L NaOH solution, cook at 90°C for 20min or more, cool, place in deionized water and soak repeatedly until Neutral, bacterial cellulose solid was obtained after freeze-drying. Take 5g of dried bacterial cellulose, add it to 100mL of sulfuric acid with a mass percentage of 35%, react in a constant temperature water bath at 50°C for 4 hours, add 800mL of deionized water to terminate the reaction, and after cooling, disperse with ultrasonic (35kHz) for 30min, and dialyze with deionized water for 3 days After drying, nanocrystalline cellulose (cellulose nanocrystals, CNCs) is obtained. Use acetic acid solution with a mass concentration of 1% as a solvent to prepare CNCs solution and collagen solution with a mass concentration of 3%; take 1g of the above CNCs solution and drop it into 9...

Embodiment 3

[0043] The method of degrading cotton cellulose with sulfuric acid in Example 1 was used to prepare nanocrystalline cellulose (cellulose nanocrystals, CNCs). Use acetic acid solution with a mass concentration of 0.5% as a solvent to prepare CNCs solution and collagen solution with a mass concentration of 2%; respectively take different volumes of the above CNCs solution and drop them into the above collagen solution, and mechanically stir for 2 hours to obtain the CNCs solution. 1wt%, 3wt%, 5wt%, 7wt% and 10wt% CNCs / collagen mixed solution, then pour the mixed solution into a polytetrafluoroethylene mold, put it in an oven at 40°C, and evaporate the solvent to make 1wt %, 3wt%, 5wt%, 7wt% and 10wt% CNCs reinforced collagen composite membranes. The swelling rate of the composite membrane was measured by weighing method, and the results were as follows: Figure 5 shown. Using a mechanical strength testing machine (Instron5543), each group of 5 parallel samples was used to meas...

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Abstract

The invention discloses a preparation method and an application of a cellulose nanocrystals (CNCs)-reinforced collagen compound substrate, which belong to the field of tissue engineering. The preparation method comprises the following steps of: preparing CNCs by adopting a method for degrading cellulose with sulfuric acid; preparing a CNCs solution and a collagen solution, and mixing the CNCs solution and the collagen solution physically; and pouring the mixed solution into a mold, and molding to obtain the CNCs-reinforced collagen compound substrate. According to the CNCs-reinforced collagen compound substrate prepared with the method, the problems of poor mechanical strength and poor hydrophilicity of the conventional collagen material are solved; and the CNCs-reinforced collagen compound substrate can be applied in the fields of wound dressings, tissue inducing regeneration membranes, soft tissue patches, tissue engineering skin, facial masks and the like. A preparation process technic provided by the invention is easy and practicable, has high repeatability, and is suitable for industrial production of CNCs-reinforced collagen compound substrates of different requirements.

Description

technical field [0001] The invention belongs to the field of tissue engineering and relates to a collagen matrix, in particular to the preparation and application of a nano-microcrystalline cellulose-reinforced collagen composite matrix. Background technique [0002] Collagen is the main component of the extracellular matrix and the most abundant structural protein in the human body, accounting for about 25% to 35% of the total protein in the body, mainly found in connective tissue. Because of the high content of collagen, connective tissue has certain structural and mechanical properties, such as tensile strength, tension, elasticity, etc., to achieve the functions of support and protection. [0003] Collagen has good biocompatibility, nutrition, repair, moisturizing, compatibility and affinity, so it is widely used in functional products such as biomedical materials, cosmetics, food and health products. Collagen has the functions of beauty (anti-wrinkle, moisturizing, whi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/32A61L15/28A61L27/48A61L27/60A61L31/12A61K8/02A61K8/64A61K8/73A61Q19/00
Inventor 张渊明郭瑞李卫昌
Owner JINAN UNIVERSITY
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