Method for preparing high-purity agaricus blazei murrill polysaccharide

Abstract

The invention discloses a method for preparing a high-purity agaricus blazei murrill polysaccharide and provides a method capable of obtaining high content of the agaricus blazei murrill polysaccharide and suitable for large-scale production. The method comprises the following steps of: adding cellulase and pectinase to an agaricus blazei murrill powder; after adding water to the agaricus blazei murrill powder and uniformly stirring the water and the agaricus blazei murrill powder, regulating the pH value of a mixture to 6.0+ / -0.1; performing enzymolysis on the mixture for 40 minutes; carrying out microwave extraction on the mixture; freezing and drying agaricus blazei murrill water extract so as to obtain a concentrated product; carrying out decolorizing and primary impurity removal treatment on an agaricus blazei murrill polysaccharide extract solution by using a macroporous adsorbing resin; and then carrying out deproteinization treatment on the agaricus blazei murrill polysaccharide extract solution by using an ion exchange resin; further purifying the agaricus blazei murrill polysaccharide after deproteinization by using an ultrafiltration technology, so as to eliminate micromolecular water soluble substances; pre-freezing an agaricus blazei murrill polysaccharide ultrafiltrate to -60 DEG C; maintaining the agaricus blazei murrill polysaccharide ultrafiltrate at -60 DEG C for at least 4 hours; and drying the agaricus blazei murrill polysaccharide ultrafiltrate at -45 DEG C in a vacuum state so as to obtain the agaricus blazei murrill polysaccharide. Through the adoption of a microwave synergistic complex enzyme method, a resin deproteinization technology, an ultrafiltration process purification and freeze-drying technology, which are combined with each other, the purity of the agaricus blazei murrill polysaccharide reaches 90%.

Description

Technical field [0001] The invention relates to the technical field of health food processing, in particular to a method for preparing high-purity Agaricus blazei polysaccharides. Background technique [0002] Agaricus Blazei Murill, also known as Brazilian mushroom, is a kind of medicinal and edible fungus to be developed in my country. It is native to Brazil, Peru and other places in southern North America. Each 100 grams of dried Agaricus blazei mushroom contains 40%-45% protein, 38%-45% sugar, 3%-4% crude fat, 6.73% crude fiber, and its rich vitamins and trace elements. As food full of nutrition. Agaricus blazei is rich in biologically active substances, which can be used as medicines and health products; the main biologically active substances include polysaccharides, sterols, nucleic acids, unsaturated fatty acids, dietary fibers, etc. According to Japanese reports, the anti-cancer effect of Agaricus blazei It is significantly better than other 14 kinds of large fungi wit...

AI Technical Summary

Problems solved by technology

[0005] At present, Agaricus blazei polysaccharides in the domestic and foreign markets are mainly specifications with a content of 10%-50%, and the extraction method is mostly extracted by hot water extraction (the main problem is low extraction efficiency and poor stability), while the polysaccharide purification method usually uses ethanol precipitation method, Sevgag method and trichloroacetic acid method, the main disadvantage is the introduction and residue of organic solvents (only the content of the prior art can be written in the background technology, and the content related to the invention is written in the content of the invention)

[0006] Products with low content specifications greatly limit the application of Agaricus blazei polysaccharides in pharmaceutical products

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