Hydrogel micro-fluidic chip and processing method thereof

Abstract

The invention provides a hydrogel micro-fluidic chip, comprising a substrate layer, a chip layer and a gland layer, wherein the chip layer is formed by solidifying PEG (polyethylene glycol) base hydrogel pre-polymer and composed of an upper layer unit and a lower layer unit; a first layer unit comprises a micro-well array used for capturing cells and fixing and cultivating the captured cells; a second layer unit comprises a direct current channel used for pouring cell culture solution and rectangular grooves used for collecting and detecting cell metabolin; the micro-well array of a first layer chip unit is overlapped with the direct current channel area of a second layer chip unit; the rectangular grooves of the first layer chip unit and the second layer chip unit are overlapped; the chip layer is arranged between the substrate layer and the gland layer; and the gland layer is provided with a sample inlet and a sample outlet which are communicated with the direct current channel and the rectangular grooves. According to the invention, a processing method for the micro-fluidic chip is also provided; the hydrogel micro-fluidic chip is manufactured by poly(ethylene glycol) diacrylate (PEGDA), thus, a chip is manufactured more quickly and efficiently; and the processing method has practical importance at aspects of drug research and drug selection.

Description

technical field [0001] The invention belongs to the field of biomedicine and provides a microfluidic chip, in particular to a microfluidic chip used for cell culture and metabolite detection. Background technique [0002] Cells are the basic unit of the morphological structure and life activities of organisms. To understand the laws of life activities of organisms, people must base their research on cells, investigate the structure and function of cells, and explore the life activities of cells. Cell culture is just able to realize this purpose. [0003] With the development of micro-manufacturing technology, microfluidic chip has gradually become an emerging platform for cell culture. Compared with the commonly used methods in the laboratory, the operation and analysis of cells on the microfluidic chip has many advantages: the size of the microchannel is equivalent to the size of the cell, and the perfusion culture method used by the chip is closer to the physiological sta...

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Problems solved by technology

[0005] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a hydrogel microfluidic chip for cell culture and metabolite detection, which solves the difficulty in simulating the growth environment of cells in the body due to the single function of the microfluidic chip in the prior art , which is not conducive to real-time collection and detection by the outside world

[0006] The invention also provides a fast, simple and low-cost chip manufacturing process, which solves the complicated problem of the existing microfluidic chip processing technology

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