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Method for preparing inactivated vaccine of anti-Listonella-anguillarum egg yolk antibody

A technology of Listonella eel and inactivated vaccine is applied in the field of inactivated vaccine preparation method of egg yolk antibody to achieve the effects of improving economic benefit, enhancing immunity and increasing yield

Inactive Publication Date: 2012-12-26
HUAIHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most farmers use chemically synthesized drugs or antibiotics to control the occurrence and prevalence of vibriosis in aquaculture animals, and there is a lack of an easy-to-use immune system against Listonia anguillarum that is suitable for juveniles of aquaculture animals in the market. preparation

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  • Method for preparing inactivated vaccine of anti-Listonella-anguillarum egg yolk antibody

Examples

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Effect test

Embodiment 1

[0017] Example 1, a method for preparing an inactivated vaccine for preparing yolk antibodies against Listonia anguillarum, the method uses the pathogenic Listonia anguillarum BH1 ( Listonella anguillarum BH1) was inactivated by formaldehyde solution as an immunogen, white oil, Span 80 and aluminum stearate were used as oil emulsion, and astragalus extract was used as an immune enhancer, and emulsified to prepare an oil emulsion inactivated vaccine; the specific steps are as follows:

[0018] (1) Inoculate the pathogenic Listonia eel BH1 strain on common nutrient agar broth medium, culture on a shaker at 30°C for 24 hours, add formaldehyde with a final volume concentration of 0.3% at 28°C for 48 hours, and centrifuge to obtain inactivated bacteria body;

[0019] (2) Soak Astragalus membranaceus in distilled water for 60 minutes, decoct, filter, concentrate to a final concentration of 1 g / mL of the original drug, and obtain Astragalus membranaceus extract;

[0020] (3) Heat ...

Embodiment 2

[0023] Example 2, a method for preparing an inactivated vaccine for preparing yolk antibodies against Listonia anguillarum, the method uses the pathogenic Listonia anguillarum BH1 ( Listonella anguillarum BH1) was inactivated by formaldehyde solution as an immunogen, white oil, Span 80 and aluminum stearate were used as oil emulsion, and astragalus extract was used as an immune enhancer, and emulsified to prepare an oil emulsion inactivated vaccine; the specific steps are as follows:

[0024] (1) Inoculate the pathogenic Listonia eel BH1 strain on ordinary nutrient agar broth medium, culture it on a shaker at 29°C for 24 hours, add formaldehyde with a final volume concentration of 0.3% at 28°C for 48 hours, and centrifuge to obtain inactivated bacteria body;

[0025] (2) Soak Astragalus membranaceus in distilled water for 60 minutes, decoct, filter, concentrate to a final concentration of 1 g / mL of the original drug, and obtain Astragalus membranaceus extract;

[0026] (3) ...

Embodiment 3

[0029] Example 3, a method for preparing an inactivated vaccine for preparing yolk antibodies against Listonia anguillarum, which uses the pathogenic Listonia anguillarum BH1 ( Listonella anguillarum BH1) was inactivated by formaldehyde solution as an immunogen, white oil, Span 80 and aluminum stearate were used as oil emulsion, and astragalus extract was used as an immune enhancer, and emulsified to prepare an oil emulsion inactivated vaccine; the specific steps are as follows:

[0030] (1) Inoculate the pathogenic Listonia eel BH1 strain on ordinary nutrient agar broth medium, culture it on a shaker at 28°C for 24h, add formaldehyde with a final volume concentration of 0.3% at 28°C for 48h, and centrifuge to obtain the inactivated bacteria body;

[0031] (2) Soak Astragalus membranaceus in distilled water for 45 minutes, decoct, filter, concentrate to a final concentration of 1 g / mL of the original drug, and obtain Astragalus membranaceus extract;

[0032] (3) Heat the wh...

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Abstract

The invention relates to a method for preparing inactivated vaccine of anti-Listonella-anguillarum egg yolk antibody. The method comprises the following steps: inactivating and centrifuging etiology Listonella-anguillarum BH1 so as to obtain inactivated thallus; preparing Astragalus mongholicus leachate; preparing oil phase by white oil, aluminium stearate and span-80; preparing vaccine water phase by the inactivated thallus and the Astragalus mongholicus leachat; finally, mixing the oil phase with the water phase according to the volume ratio of 2:1, emulsifying and milling for 5 minutes so as to obtain the Vibrio parahaemolyticus oil emulsion inactivated vaccine in which Astragalus mongholicus and oil white are used as united adjuvant. By adoption of the vaccine, the anti-Listonella-anguillarum egg yolk antibody can be obtained; by adoption of the anti-Listonella-anguillarum egg yolk antibody, the immunity of marine fishes such as cynoglossus semilaevis to etiology Listonella-anguillarum is improved, so that the survival rate is improved, the yield is improved, and the economic benefit of culture production of marine fishes such as cynoglossus semilaevis is improved. The problem that the Listonella-anguillarum caused diseases frequently occur in the culture production of marine fishes such as cynoglossus semilaevis, is solved.

Description

technical field [0001] The invention relates to a preparation method of an inactivated vaccine, in particular to an inactivated vaccine preparation method for preparing egg yolk antibody against Listonia anguillarum. Background technique [0002] Listonella anguillarum has long been recognized as a pathogenic bacterium of aquaculture animals. The bacterium was first isolated from an eel with "red plague" in 1893 by Canestrini in Italy. It is known to cause steelhead trout, salmon, turbot, sweetfish, eel, Pacific salmon, yellowtail, jack mackerel, sea bream, perch, plaice, cod, mackerel, various tropical freshwater fish As well as shrimps, crabs, oysters, clams, abalone and other aquaculture animals, lethal vibriosis occurs. Diseases caused by Listeria anguillarum have caused great losses to the production of aquatic animals. Therefore, effective prevention of diseases caused by Listeria anguillarum to minimize its harmfulness will significantly To improve the economic bene...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/395A61P31/04
Inventor 张晓君毕可然陈丽闫斌伦秦国民
Owner HUAIHAI INST OF TECH
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