Test strip for one-step identification of foot-and-mouth disease virus infection and vaccine-immunized animals and preparation method thereof

Abstract

The invention relates to a test strip for rapidly identifying a foot-and-mouth-disease virus infected and vaccine immunized animal and a preparation method of the test strip. The test strip comprises a supporting layer and an adsorbing layer; the adsorbing layer is attached to the supporting layer; the adsorbing layer comprises a sample adsorbing fiber layer, a gold-labeled antibody fiber layer and a cellulose membrane layer sequentially arranged on the testing end and further comprises a water absorbing material layer arranged on the handle end; a detection blot and a control blot are arranged on the cellulose membrane layer; the colloidal gold labeled staphylococcus protein A is adsorbed on the gold-labeled antibody fiber layer; the detection blot is printed by one or two of a pig foot-and-mouth-disease virus protein structural VPI antigen and a non-structural protein 3ABC antigen which are expressed and purified by an escherichia coli expression system; and the control blot is printed by using a goat anti- or rabbit anti-SPA IgG antibody solution. The test strip is high in specificity, high in sensitivity, simple, direct, exact, wide in application scope, low in cost, capable of being used by professionals or non-professionals for detection at any time and any place and beneficial to popularization and application.

Description

technical field [0001] The invention relates to a device for detecting foot-and-mouth disease virus, in particular to a test strip for quickly identifying foot-and-mouth disease virus infection and vaccine-immunized animals and a preparation method thereof. Background technique [0002] Foot-and-mouth disease (FMD) is a severe infectious disease of cloven-hoofed animals caused by foot-and-mouth disease virus (FMDV). FMDV has the characteristics of polymorphism, variability, wide range of hosts, and strong infectivity. Once it occurs, it will become epidemic and large-scale outbreaks. The outbreak and prevalence of FMD often cause huge losses to the affected areas. In addition to the direct economic losses caused by animal deaths, there are also stagnation of meat and milk production during animal illness, long-term decline in meat and milk production after recovery, and seed value. Loss and other major losses. What's more serious is that FMD is a severe infectious disease,...

AI Technical Summary

Problems solved by technology

This technique is accurate and reliable, but the sensitivity is poor, and there is complement activity in some samples, which affects the detection effect, and requires a specialized laboratory

(2) Virus neutralization test: The interaction between the virus and specific serum neutralizing antibodies is used to make the virus lose its ability to infect susceptible animals and sensitive cells. This method must use live viruses, which cannot be operated by ordinary laboratories

The ELISA method has the advantages of specificity and sensitivity in determination, but the operation is complex, time-consuming and laborious, requiring specific equipment and professional technical personnel, and it is difficult to popularize at the grassroots level

At the same time, the liquid-phase blocking ELISA method requires the participation of inactivated viruses, so there are risks such as incomplete virus inactivation or virus escape.

In addition, in recent years, with the popularization and application of pig O-type FMD synthetic peptide vaccine, it has gradually occupied the main market. It is difficult for traditional liquid-phase blocking ELISA to make accurate detection results for peptide vaccine antibodies.

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