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Preparation method of high-lysine fermentation and detoxification cottonseed meal

A technology of high lysine and cottonseed meal, which is applied in application, animal feed, animal feed, etc., can solve the problems of unbalanced amino acid ratio, animal growth retardation, stunting, etc., and achieve wide application of finished products, speed up, and improve palatability effect

Inactive Publication Date: 2012-12-05
XINJIANG XIPU BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At the same time, detoxified cottonseed meal can be used as a safe protein source, but its nutritional components, especially the unbalanced ratio of amino acids, such as its high content of arginine, are harmful to breastfeeding and young children. Animals are beneficial, however, the lack of lysine, methionine, etc. is a major disadvantage of it
If it is fed alone as feed, lysine must be added separately, otherwise it may cause growth retardation or even stunted growth of animals
[0005] In addition, in the production of lysine, Brevibacterium flavum and Corynebacterium are generally used as the main production bacteria. It is also reported that yeast can be used to produce lysine, but the use of yeast to produce high-lysine detoxified cotton meal rarely reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A preparation method for high-lysine fermented detoxified cottonseed meal, comprising the steps of:

[0033] 1) Activation of strains:

[0034] a. Inoculate the cells of Candida tropicalis, Candida utilis, and Saccharomyces cerevisiae into the Erlenmeyer flasks filled with yeast liquid culture medium, and culture them with shaking at 30°C for 36 hours until various bacterial liquids Concentration is 10 8 ~10 9 cfu / mL;

[0035] in:

[0036] The concentration of each component in the yeast seed culture medium is: peptone 4.55g / L, yeast extract 14g / L, sodium chloride 3.5g / L, glucose 9g / L, and the pH value is 7.0.

[0037] The concentration of each component in the Lactobacillus seed medium is: peptone 4.5g / L, glucose 3.5 g / L, sodium chloride 2.5g / L, potassium dihydrogen phosphate 2.5g / L, dipotassium hydrogen phosphate 2.5 g / L, Starch 1.8 g / L, magnesium chloride 0.2 g / L, calcium sulfate 0.05 g / L.

[0038] b. Use a pipette to draw 100 μL of liquid Lactobacillus cells ...

Embodiment 2

[0049] A preparation method for high-lysine fermented detoxified cottonseed meal, comprising the steps of:

[0050] 1) Activation of strains:

[0051] a. Inoculate the cells of Candida tropicalis, Candida utilis, and Saccharomyces cerevisiae into the Erlenmeyer flasks filled with yeast liquid culture medium, and culture them with shaking at 30°C for 40 h until various bacterial liquids Concentration is 10 8 ~10 9 cfu / mL;

[0052] in:

[0053] The concentration of each component in the yeast seed culture medium is: peptone 4.5g / L, yeast extract 14g / L, sodium chloride 5g / L, glucose 10g / L, and the pH value is 7.0.

[0054] The concentration of each component in the Lactobacillus seed medium is: protein 4 g / L, glucose 4 g / L, sodium chloride 2 g / L, potassium dihydrogen phosphate 2 g / L, dipotassium hydrogen phosphate 2 g / L, starch 1.5 g / L, magnesium chloride 0.2 g / L, calcium sulfate 0.05 g / L.

[0055] b. Use a pipette to draw 100 μL of liquid Lactobacillus cells into a 250 mL...

Embodiment 3

[0066] A preparation method for high-lysine fermented detoxified cottonseed meal, comprising the steps of:

[0067] 1) Activation of strains:

[0068] a. Inoculate Candida tropicalis, Candida utilis, and Saccharomyces cerevisiae into the triangular flasks filled with yeast liquid culture medium respectively, and culture them with shaking at 37°C until the concentration of various bacterial solutions is 10 8 ~10 9 cfu / mL;

[0069] in:

[0070] The concentration of each component in the yeast seed culture medium is: peptone 5.5g / L, yeast extract 15g / L, sodium chloride 5g / L, glucose 10g / L, and the pH value is 7.0.

[0071] The concentration of each component in the Lactobacillus seed medium is: protein 5g / L, glucose 5 g / L, sodium chloride 3g / L, potassium dihydrogen phosphate 3g / L, dipotassium hydrogen phosphate 3 g / L, starch 2g / L L, magnesium chloride 0.2 g / L, calcium sulfate 0.05 g / L.

[0072] b. Use a pipette to draw 100 μL of liquid Lactobacillus cells into a 250 mL Erl...

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Abstract

The invention discloses a preparation method of high-lysine fermentation and detoxification cottonseed meal and aims at providing the preparation of high-lysine fermentation and detoxification cottonseed meal, which has the advantages of simultaneously realizing the cottonseed meal detoxification and improving the nutrient substance content in the cottonseed meal, particularly the lysine content. The preparation method has the main technical scheme that the method sequentially comprises the following steps of 1) bacterial strain activation: candida tropicalis, candida utilis and saccharomyces cerevisiae are respectively inoculated to a saccharomycete culture medium, and lactobacilli is inoculated into a culture medium containing liquid lactobacilli; 2) preparation of seeds for production; 3) fermentation substrate preparation; and 4) fermentation substrate inoculation. The preparation method belongs to the technical field of feed preparation.

Description

technical field [0001] The invention relates to a method for preparing fermented cottonseed meal, in particular to a method for preparing fermented detoxified cottonseed meal with high lysine content, and belongs to the technical field of feed preparation. Background technique [0002] With the improvement of people's living standards, the consumption of meat is increasing day by day, which promotes the vigorous development of my country's aquaculture industry to a certain extent. However, in our country, protein feed resources are seriously lacking, and will become a bottleneck restricting the development of animal husbandry in our country for a long time. Studies have found that cottonseed meal is rich in protein, which can be used as a good source of protein feed, but at the same time, cottonseed meal also contains free gossypol, which is toxic to livestock and poultry. Gossypol poisoning is manifested as loss of appetite, weight loss, General edema, weakness, dyspnea, h...

Claims

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Application Information

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IPC IPC(8): A23K1/14
Inventor 王华荣恽辉余忠丽赵大伟程林春李伟霞张玲耿玉静
Owner XINJIANG XIPU BIOLOGICAL SCI & TECH
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