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Virus removing method for lily tissue culturing seedling by treatment with chemical agent

A technology of chemical agent and lily group, which is applied in the field of plant tissue culture, can solve the problems of great difference in treatment time and difficulty in determining the effect of plant treatment, etc., and achieve the effect of good detoxification effect, bright color and strong growth potential of plants

Inactive Publication Date: 2007-01-31
潘利军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical agents that can be used at this stage include purine and pyrimidine compounds, amino acids, antibiotics, ribavirin, etc. Due to different plant species, there are great differences in the selection of agents, concentration deployment and treatment time, and the treatment effects of different agents on different plants It is also difficult to determine; it has been reported that ribavirin can be used to carry out detoxification treatment to caryophyllum plants, the drug concentration is 5mg / L, and the detoxification rate of CMV (cucumber mosaic virus) is 80%; and for the chemical medicament detoxification process of lily There is no detailed report yet, how to ensure the normal development of lily and better detoxification effect is the key to the detoxification treatment of lily chemical agents

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1 The method of detoxification of lily tissue culture seedlings treated with chemical agents:

[0014] 1) Culture medium preparation: add ribavirin to the sterilized but not yet condensed MS medium under aseptic conditions after filter sterilization (see Table 2 for the composition of the medium);

[0015] 2) Induction and multiplication of lily tissue culture seedlings: when lily buds are 5-10 cm long, take the shoot tip meristem, inoculate the explants in the above MS medium, add BA (6-benzylaminopurine) 0.5mg / l, NAA (naphthalene acetic acid) 0.5mg / l and sucrose 30g / l carry out induction culture; 12~14 hours (this embodiment is 14 hours) light conditions every day at 2000~3000LX (this embodiment is 2000LX) Under the temperature condition of 20~26 ℃, after 30~40 days (the present embodiment is 30 days), many compact adventitious buds are directly induced; observe its induction rate and detoxification effect;

[0016] concentration of ribavirin

(μ...

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PUM

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Abstract

A chemical method for detoxicating the tissue-cultured seedlings of lily features that its culture medium for tissue culture contains ribavirin (200-505 mu mol / L). Its advantages are high survival rate and high detoxicating effect.

Description

technical field [0001] The invention relates to plant tissue culture, in particular to a detoxification method for lily tissue culture seedlings treated with chemical agents. Background technique [0002] Plant tissue culture refers to the method of inoculating a part of a plant, such as a small stem, a small leaf, or even a cell, on a medium under in vitro conditions to make them regenerate into a complete plant. The steps of tissue culture include the preparation of culture medium and the disinfection of inoculated materials, inoculation, plant induction, rooting and transplanting. [0003] Element Use concentration (mg / L) Large amount of yuan white Potassium nitrate KNO 3 1900 Ammonium nitrate NH 4 NO 3 1650 Potassium dihydrogen phosphate KH 2 PO 4 170 Magnesium Sulfate MgSO 4 ·7H 2 o 370 Calcium Chloride CaCl 2 2H 2 o 440 Trace elements whi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 潘利军
Owner 潘利军
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