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Cell inactivated vaccine, egg yolk antibody injection, and preparation method of cell inactivated vaccine

A technology of inactivated vaccine and egg yolk antibody, applied in the field of cell inactivated vaccine, egg yolk antibody injection and preparation thereof, can solve the problems of time-consuming and labor-intensive, reduce production steps, reduce the loss of antibody, and reduce the probability of disease Effect

Inactive Publication Date: 2012-10-10
河南后羿生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, in actual production, animal blood collection is not only a great stress on the animal itself, but also time-consuming and labor-intensive. Large-scale production is impractical, and it is much more convenient to use laying hens to produce antibodies

Method used

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  • Cell inactivated vaccine, egg yolk antibody injection, and preparation method of cell inactivated vaccine
  • Cell inactivated vaccine, egg yolk antibody injection, and preparation method of cell inactivated vaccine
  • Cell inactivated vaccine, egg yolk antibody injection, and preparation method of cell inactivated vaccine

Examples

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Embodiment 1

[0025] Example 1: Preparation of porcine circovirus cell inactivated vaccine in this example. The disease material was collected from pig farms with severe porcine circovirus disease in Shandong area. After clearing and filtering through a 0.22 μm filter membrane, a sensitive monolayer of PK-15 cells was inoculated. After the PK-15 cells were continuously passaged for 6 generations, part of the cells were frozen and thawed to extract the DNA of the PCV-2 virus, and the target gene was amplified with PCR primers specific to the PCV-2 virus ORF1 gene. The amplified product was cloned into the PMD-18T vector for sequencing, identified and named SD strain. Multiple viral epitopes with antigenic immune activity were screened out in this strain, and five antigenic epitopes were finally selected after specific primer PCR, product electrophoresis, sequencing, cloning, purification, and detection of protein immune activity of each epitope. site, each antigen site is connected by splic...

Embodiment 2

[0047] Embodiment 2: The preparation of porcine circovirus cell inactivated vaccine of the present embodiment collects disease material from pig farm that serious porcine circovirus takes place in Shandong area, and disease material is after homogenization, freeze-thaw repeatedly 3 times, high-speed centrifugation, take After the supernatant was filtered through a 0.22 μm filter membrane, a sensitive monolayer of PK-15 cells was inoculated. After the PK-15 cells were continuously passaged for 6 generations, part of the cells were frozen and thawed to extract the DNA of the PCV-2 virus, and the target gene was amplified with PCR primers specific to the PCV-2 virus ORF1 gene. The amplified product was cloned into the PMD-18T vector for sequencing, identified and named SD strain. Multiple viral epitopes with antigenic immune activity were screened out in this strain, and five antigenic epitopes were finally selected after specific primer PCR, product electrophoresis, sequencing, ...

Embodiment 3

[0069] Embodiment 3: The preparation of porcine circovirus cell inactivated vaccine of this embodiment collects disease material from pig farm that serious porcine circovirus takes place in Shandong area, and disease material is after homogenization, freeze-thaw repeatedly 3 times, high-speed centrifugation, take After the supernatant was filtered through a 0.22 μm filter membrane, a sensitive monolayer of PK-15 cells was inoculated. After the PK-15 cells were continuously passaged for 6 generations, part of the cells were frozen and thawed to extract the DNA of the PCV-2 virus, and the target gene was amplified with PCR primers specific to the PCV-2 virus ORF1 gene. The amplified product was cloned into the PMD-18T vector for sequencing, identified and named SD strain. Multiple viral epitopes with antigenic immune activity were screened out in this strain, and five antigenic epitopes were finally selected after specific primer PCR, product electrophoresis, sequencing, cloning...

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Abstract

The invention relates to a cell inactivated vaccine, an egg yolk antibody injection and a preparation method of the cell inactivated vaccine. The cell inactivated vaccine comprises a porcine circovirus antigenic epitope and a goose circovirus antigenic epitope. The preparation method of the cell inactivated vaccine comprises the following steps: firstly screening high-pathogenicity strains from the porcine circovirus, then selecting a plurality of virus epitopes having antibody immunocompetence from the strains, inserting the point of the porcine circovirus antigenic epitope into a goose circovirus genome by technologies such as purification and clone, and then carrying out viral multiplication in a large scale to obtain the cell inactivated vaccine. The invention also discloses the egg yolk antibody injection taking the cell inactivated vaccine as an antibody at the same time, the injection can be used for treating porcine circovirus diseases, takes effect quickly and has remarkable effects, meanwhile, a small dose can also be used for preventing the occurrence of the porcine circovirus diseases, so that the incidence rate is reduced and the economic benefit is improved. Meanwhile, the egg yolk antibody can also be used for treating the goose circovirus diseases. The preparation has lower stimulation on a human body, the working efficiency is further improved, and the feeding cost is lowered.

Description

technical field [0001] The invention belongs to the technical field of immune prevention, relates to a cell inactivated vaccine, and also relates to an egg yolk antibody injection made from the cell inactivated vaccine and a preparation method thereof. Background technique [0002] Porcine circovirus type 2 (PVC-2) is the main pathogen of multisystem failure syndrome and nephrotic dermatitis syndrome in weaned piglets. At the same time, the disease can also cause immunosuppression, which is easy to cause secondary or concurrent infection of other diseases, and has high The characteristics of incidence, high prevalence, high pathogenicity, and high mortality have caused huge economic losses to the pig industry. The pathogenic mechanism of PVC-2 is still unclear, and its destruction of the pig immune system is considered to be the main cause of related pig diseases. Due to the decline of immunity, pigs are vulnerable to other pathogens during stress and pathogenic infection. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/12A61K39/42A61K9/08A61P31/20C07K16/08C07K16/02
Inventor 吴红云郭俊清徐进
Owner 河南后羿生物工程股份有限公司
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