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Method for extracting medicine for treating human cardiovascular diseases from thalictrum robustum

A thick and strong Tang pine grass, crude extraction technology, applied in cardiovascular system diseases, pharmaceutical formulations, drug combinations and other directions, can solve the problems of high total alkaloid impurity content, high blood pressure dosage, low blood pressure activity, etc., and achieves high prevention and control. Blood pressure disease, small effect of activity, effect of simple steps

Inactive Publication Date: 2012-10-10
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] This method is not very ideal for extracting the active ingredients that have the effect of preventing and treating hypertension, and the activity of the total alkaloids obtained in preventing and treating hypertension is not high.
[0004] Also, as disclosed in CN102008558A, the extraction method of Siye Tangsongcao adopts decoction, decompression concentration and other methods, and the impurity content in the total alkaloids extracted by this method is too high, which leads to excessive dosage when it is used in the prevention and treatment of hypertension.

Method used

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  • Method for extracting medicine for treating human cardiovascular diseases from thalictrum robustum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Put 200 grams of pulverized thick tang pine grass leaf powder in an ultrasonic extractor, the ethanol concentration is 95%, the weight ratio of thick tang pine grass and solvent is 1: 10, soak for 50min, ultrasonic frequency 25kHz, extraction temperature 50 ℃ , the extraction time is 60min, the extract after ultrasonic extraction is suction-filtered, and the supernatant is concentrated under reduced pressure at 50°C to obtain the crude extract of Pinus tangensis;

[0021] (2) Add water 5 times of the weight of the crude extract to the crude extract of Pinus tangus and stir into a crude extract suspension, pass the suspension through 001×7 cation exchange resin, and use 4% aqueous sodium hydroxide solution to cation Exchange the resin for analysis to obtain an analysis solution; acidify the analysis solution with hydrochloric acid to pH=5.4, and concentrate under reduced pressure at 50° C. to obtain a total alkaloid extract.

Embodiment 2

[0023] (1) Put 200 grams of pulverized thick tang pine grass leaf powder in an ultrasonic extractor, the ethanol concentration is 99%, the weight ratio of thick tang pine grass and solvent is 1: 15, soak for 60min, ultrasonic frequency 35kHz, extraction temperature 60 ℃ , the extraction time was 80min, the extract after ultrasonic extraction was subjected to suction filtration, and the clear liquid was concentrated under reduced pressure at 60°C to obtain the crude extract of Pine Grass Tang;

[0024] (2) Add 10 times the water of the crude extract weight to the crude extract of Pinus tangerae and stir into a crude extract suspension, pass the suspension through the TS-92 model cation exchange resin, and use 4-8% sodium hydroxide Analyze the cation exchange resin with the aqueous solution to obtain the analysis solution; acidify the analysis solution with hydrochloric acid to pH = 5.8, and concentrate under reduced pressure at 60°C to obtain the total alkaloid extract.

experiment Embodiment 1

[0030] The total alkaloid extracts obtained in Examples 1-2 and Comparative Examples 3-4 were diluted with 5 times the mass of distilled water, and then injected into the duodenum of drug-anesthetized rats at a dose of 0.5ml / 100g rat body weight. Intestinally, systolic blood pressure, diastolic blood pressure, heart rate, onset time and maintenance time of said heart rate were measured in rats. The specific results are as follows:

[0031]

[0032] All data above P<0.05

[0033] For the same stout tang pine grass leaf powder, different extraction methods are used to carry out experiments, and it is found that the extract obtained by the extraction method of the present invention has a good effect in preventing and treating hypertension, and the impact time on heart rate is very short, and the side effects small.

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Abstract

The invention relates to a method for extracting medicine for treating human cardiovascular diseases from thalictrum robustum. The method comprises the following steps that thalictrum robustum is subjected to ultrasonic extraction and cation exchange resin desorption, wherein the solvent of the ultrasonic extraction is ethanol, the ultrasonic frequency is 25 to 35 kHz, the cation exchange resin desorption adopts sodium hydroxide water solution, and hydrochloric acid is adopted for desorption in desorption solution. According to the method, through ultrasonic extraction and cation exchange resin active ingredient adsorption, the finally obtained extracts can be used for effectively preventing and treating hypertensive diseases. The method has the advantages that the steps are simple, the influence on the activity of active ingredients is small, and the active ingredients of the extracts cannot be weakened, Compared with the existing extracting method, the method obtains the outstanding progress.

Description

technical field [0001] The invention belongs to the field of medicine preparation, and in particular relates to a method for extracting medicine for treating human cardiovascular diseases from Tangsongcao bracken. Background technique [0002] At present, there are many methods for extracting medicines from plants of the genus Echinacea, for example: the method disclosed in CN101278989A, which is to add Echinacea to a polar organic solvent for extraction, stir with water into a crude extract suspension, and pass through the adsorption resin successively. Separate the separation column and cation exchange resin to obtain total saponins; use 1-10% NaOH aqueous solution or organic solvent to analyze the cation exchange resin, and then acidify the analysis solution with hydrochloric acid or sulfuric acid to obtain the total alkaloid extract; the above organic Solvents include methanol, ethanol, and the like. [0003] This method is not very ideal for extracting the effective co...

Claims

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Application Information

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IPC IPC(8): A61K36/71A61P9/12
Inventor 陈峰
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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