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Culture method for inhibiting browning of bolete mycelium

A cultivation method and mycelium technology, which are applied in the directions of botanical equipment and methods, horticulture, and application, can solve the problems of easy browning of culture medium and mycelium, high price, and natural yield decline, and reduce browning. factors, the effect of shortening the culture period and reducing the degree of browning

Inactive Publication Date: 2012-10-10
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Boletus is a kind of mycorrhizal fungi that live in symbiosis with plants. Its output is severely limited by the symbiotic environment, climate of the place of production, collection and preservation methods, etc., and the output is limited.
At present, boletus is still difficult to carry out artificial cultivation, and its fruiting bodies are completely dependent on wild harvesting.
Yunnan is rich in natural resources, and the output of wild boletus is large. However, due to climate change and ecological damage in recent years, its natural output has been severely reduced and the price is expensive. It is difficult to go to the public table. Therefore, the artificial cultivation of boletus is still a The ultimate goal of scientific research workers
There are still many problems in the research on the artificial cultivation of boletus. Among them, mycelium grows extremely slowly on the artificial medium. Important factors for mass reproduction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0017] Mature fruiting bodies picked in the field with good growth, no pests, unopened umbrellas, thicker stipe, and thicker caps;

[0018] Bring the fruiting body back to the laboratory, wipe the surface of the cap and the stipe with an alcohol cotton ball on the ultra-clean table to remove the surface sediment or soil, divide the fruiting body into two from the middle of the cap, and dissect it Take the internal tissue block at the junction of the stipe and the cap with a knife, and cut into about 0.3-0.50cm 2 small pieces;

[0019] Gently embed the small tissue pieces cut above into the surface of the test tube slant induction medium (induction medium: potato 200.00g / L, glucose 20.00g / L, MgSO 4 1.00g / L, CaCl 2 1.00g / L, KH 2 PO 4 0.50g / L, NH 4 NO 3 0.40g / L, KNO 3 0.40g / L, V B1 0.10mg / L, wort juice 150 ml / L, glutamic acid 0.16 g / L, agar 13.00g / L, control the pH value to be about 5.4), culture temperature is 22~23℃, dark culture for 7 days, around the bacteria block...

example 2

[0023] Mature fruiting bodies picked in the field with good growth, no pests, unopened umbrellas, thicker stipe, and thicker caps;

[0024]Bring the fruiting body back to the laboratory, wipe the surface of the cap and the stipe with an alcohol cotton ball on the ultra-clean table to remove the surface sediment or soil, divide the fruiting body into two from the middle of the cap, and dissect it Take the internal tissue block at the junction of the stipe and the cap with a knife, and cut into about 0.3-0.50cm 2 small pieces;

[0025] Gently embed the small tissue pieces cut above into the surface of the test tube slant induction medium (induction medium: potato 200.00g / L, glucose 20.00g / L, MgSO 4 1.30g / L, CaCl 2 1.20g / L, KH 2 PO 4 0.60g / L, NH 4 NO 3 0.40g / L, KNO 3 0.40g / L, V B1 0.30mg / L, wort 150 ml / L, glutamic acid 0.20 g / L, agar 15.00 g / L, control the pH value to be about 5.4), culture temperature is 22~23℃, dark culture for 8 days, around the bacteria block The w...

example 3

[0029] Mature fruiting bodies picked in the field with good growth, no pests, unopened umbrellas, thicker stipe, and thicker caps;

[0030] Bring the fruiting body back to the laboratory, wipe the surface of the cap and the stipe with an alcohol cotton ball on the ultra-clean table to remove the surface sediment or soil, divide the fruiting body into two from the middle of the cap, and dissect it Take the internal tissue block at the junction of the stipe and the cap with a knife, and cut into about 0.3-0.50cm 2 small pieces;

[0031] Gently embed the small tissue pieces cut above into the surface of the test tube slant induction medium (induction medium: potato 200.00g / L, glucose 20.00g / L, MgSO 4 1.20g / L, CaCl 2 0.80g / L, KH 2 PO 4 0.40g / L, NH 4 NO 3 0.40g / L, KNO 3 0.40g / L, V B1 0.20mg / L, wort juice 150 ml / L, glutamic acid 0.13g / L, agar 10.00g / L, control the PH value to be about 5.4), culture temperature is 22~23℃, dark culture for 12 days, around the bacteria block...

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PUM

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Abstract

The invention relates to a culture method for inhibiting the browning of bolete mycelium and belongs to the technical field of organism (macro fungi culture). The artificial culture of bolete is difficult to realize at present, and bolete fruiting bodies completely rely on wild picking. In recent years, because of the reasons of climate change, ecological damage and the like, the natural yield isseriously reduced, and the price is high. The growth of bolete hyphae on an artificial culture medium is slow, the culture medium and the hyphae easily brown after the hyphae grow for a period of time, and the subsequent growth is influenced. According to the method provided by the invention, a strain of unopened boletus edulis fruiting body produced in Yunnan is adopted as materials, paths such as mycelium inducing, mycelium activation and the like are adopted, and a culture medium capable of inhabiting or reducing the browning is provided. The culture medium has the characteristics that ingredients are clear and simple, the cost is low, the growth of the mycelium is fast, the bacterial colony is white and clean, and the like. Important foundation is laid for the scaled culture, artificial or semi-artificial domestication and the like of the bolete mycelium.

Description

technical field [0001] The present invention relates to a kind of wild edible fungus - broad sense delicious boletus ( Boletus edulis s.l.) mycelium culture method, belongs to the field of biotechnology, specifically belongs to the large fungus mycelium culture category. Background technique [0002] Boletus ( Boletus ) belongs to Basidiomycota, Agaricomycetes, Boletales, and Boletaceae. It is a large genus widely distributed in the world. The warm regions from the Arctic to the equator are also rarely distributed in Malaysia in the tropics and Colombia in the southern hemisphere. There are currently 273 species (including subspecies, varieties and types) of this genus recorded in the database. There are 5 genera of boletus in China, among which there are about 129 distinguishable species, which are mainly distributed in southwestern regions such as Yunnan, Guizhou, and Sichuan. Boletus fruiting bodies are large in size, fleshy and delicious. It is a kind of delicious e...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 李宗菊王鹏飞张曦予李彪周文吴鹏
Owner YUNNAN UNIV
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