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Method for extracting serrate clubmoss herb huperzia total alkali

An extraction method and technology of a thousand-layer tower are applied in the field of alkaloid extraction, which can solve the problems of low recovery rate and low extraction efficiency, and achieve the effect of improving recovery rate and removing impurities.

Inactive Publication Date: 2012-07-25
重庆市秀山红星中药材开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, Patent No. 200710018515.4 "A method for extracting and separating Huperzine A from Melaleuca Tower", which simply uses organic solvents to soak and extract; A new method” is simply soaking and extracting with dilute sulfuric acid. These methods can only precipitate a small amount of alkaloids hidden in the cell wall, the recovery rate is very low, and the extraction efficiency is not high.

Method used

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  • Method for extracting serrate clubmoss herb huperzia total alkali
  • Method for extracting serrate clubmoss herb huperzia total alkali
  • Method for extracting serrate clubmoss herb huperzia total alkali

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Cleanly select 100kg of Melaleuca medicinal materials, pulverize them with a pulverizer, the particle size is 10-20 mesh, and sieve; then soak in 2000kg of 0.8% HCl solution for 60min, then use 600W ultrasonic power to assist extraction for 15min, then soak for 60min, and then Centrifuge the soaking solution on a centrifuge, take the supernatant, and soak the filter residue with 1800 kg of 0.8% HCl solution for 45 minutes, then use 600W ultrasonic power to assist extraction for 10 minutes, then soak for 45 minutes, then centrifuge the soaking solution on the centrifuge, take Supernatant, combine the two supernatants; then use a rotary evaporator to concentrate the filtrate to 1000L, add 10% ammonia water to adjust the pH value to 9, then add 17L chloroform to mix with the concentrated solution, extract 4 times, and combine the chloroform phases , and then extracted once; concentrated to 100L with a rotary evaporator to obtain Huperzine total alkaloids in chloroform conce...

Embodiment 2

[0024] Cleanly select 100kg of Melaleuca medicinal materials, pulverize with a pulverizer, the particle size is 10-20 mesh, and sieve; then soak in 2500kg of 0.8% HCl solution for 60min, then use 600W ultrasonic power to assist extraction for 15min, then soak for 60min, and then Centrifuge the soaking solution on a centrifuge, take the supernatant, and soak the filter residue with 2200 kg of 0.8% HCl solution for 45 minutes, then use a 600W power ultrasonic to assist extraction for 10 minutes, then soak for 45 minutes, then centrifuge the soaking solution in a centrifuge, and take Supernatant, combine the two supernatants; then use a rotary evaporator to concentrate the filtrate to 1000L, add 10% ammonia water to adjust the pH value to 9, then add 17L chloroform to mix with the concentrated solution, extract 4 times, and combine the chloroform phases , and then extracted once; concentrated to 100L with a rotary evaporator to obtain Huperzine total alkaloids in chloroform concen...

Embodiment 3

[0026] Cleanly select 100kg of melaleuca medicinal materials, pulverize them with a pulverizer, the particle size is 10-20 mesh, and sieve; then soak in 2200kg of 0.8% HCl solution for 60min, then use 600W ultrasonic power to assist extraction for 15min, then soak for 60min, and then Centrifuge the soaking solution on a centrifuge, take the supernatant, and soak the filter residue with 2000 kg of 0.8% HCl solution for 45 minutes, then use a 600W power ultrasonic to assist extraction for 10 minutes, then soak for 45 minutes, then centrifuge the soaking solution on the centrifuge, take Supernatant, combine the two supernatants; then use a rotary evaporator to concentrate the filtrate to 1000L, add 10% ammonia water to adjust the pH value to 9, then add 17L chloroform to mix with the concentrated solution, extract 4 times, and combine the chloroform phases , and then extracted once; concentrated to 100L with a rotary evaporator to obtain Huperzine total alkaloids in chloroform con...

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Abstract

The invention relates to a method for extracting serrate clubmoss herb huperzia total alkali. The method comprises the following steps of: dilute acid soaking, ultrasonic auxiliary extract, extract, re-extraction edulcoration and the like. The method for extracting the serrate clubmoss herb huperzia total alkali has the benefits of soaking and extracting by utilizing acid water, converting natural base into natural alkali salt to dissolve out, carrying out the ultrasonic auxiliary extract during extraction to crack cell walls of serrate clubmoss herb so as to greatly dissolve the natural base in soaking liquid and improve a recovery rate. According to the method for extracting the serrate clubmoss herb huperzia total alkali, disclosed by the invention, based on a test, the huperzia total alkali is extracted, thus the recovery rate can reach 98.3% and is improved, and a limit resource is saved; in addition, the extract and re-extraction are carried out by the dilute acid and dilute base so that impurities can be greatly removed; and based on the test, the purity of the huperzia total alkali extracted by the method disclosed by the invention can reach 28.1%.

Description

technical field [0001] The invention relates to an extraction method of alkaloids, in particular to an extraction method of total alkaloids from Melaleuca huperzine. Background technique [0002] The Melaleuca Pagoda is a fern of the genus Huperzia in the family Huperzaceae, also known as Huperzia serrata and King of Help. It is mainly distributed in the Northeast, the Yangtze River Basin and Fujian, Guangdong, Guangxi, Yunnan, Guizhou and other provinces. Folks often use the whole herb as medicine, which is poisonous and used for reducing fever, hemostasis, reducing swelling and detoxification, and traumatic injuries. The alkaloids huperzine A (HupA) and huperzine B (HupB) contained in Melaleuca are acetylcholinesterase inhibitors with the advantages of low toxicity, high efficiency, reversibility and high selectivity. They are useful for the treatment of myasthenia gravis, It has a good curative effect on middle-aged and elderly dementia and improving memory, and has a c...

Claims

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Application Information

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IPC IPC(8): A61K36/36A61P21/04A61P25/28
Inventor 赵勇彪祝利杨再江向廷军何全慧
Owner 重庆市秀山红星中药材开发有限公司
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