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Method for designing and preparing electrochemical detection-microfluidic multichannel chip based on self-assembled monolayer technology

A self-assembly technology and microfluidic channel technology, which is applied in the field of electrochemical detection-microfluidic multi-channel chip design and preparation, can solve the problem of single application of microelectrodes, achieve small sample consumption, expand application fields, cost reduction effect

Inactive Publication Date: 2012-07-18
EAST CHINA UNIV OF SCI & TECH
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  • Description
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  • Application Information

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Problems solved by technology

[0004] So far, most microfluidic chips based on electrochemical detection have focused on the design of microchannels, and the application of microelectrodes is relatively single. There is still a lot of room for development in the design and preparation of microelectrodes.

Method used

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  • Method for designing and preparing electrochemical detection-microfluidic multichannel chip based on self-assembled monolayer technology
  • Method for designing and preparing electrochemical detection-microfluidic multichannel chip based on self-assembled monolayer technology
  • Method for designing and preparing electrochemical detection-microfluidic multichannel chip based on self-assembled monolayer technology

Examples

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Effect test

Embodiment 1

[0039] Detection of Cu in solution using self-assembled L-cysteine 2+ ion concentration.

[0040] L-cysteine ​​(L-CySteine) is one of the 20 important amino acids in nature and has good electrochemical activity. At the same time, the L-cysteine ​​molecule contains a very active sulfhydryl group (-SH), which is easy to adsorb on the surface of the gold electrode by forming Au-S bonds to form a self-assembled film. According to a number of literature reports, L-cysteine ​​for Cu 2+ The ions coordinate very well, resulting in selective electrical signals.

[0041] In this experiment, L-cysteine ​​was self-assembled on the surface of gold electrodes through microchannels, and then the 2+ Electrochemical detection of ions in the solution to be tested. In this experiment, an 8-electrode chip and an 8-electrode microchannel were used.

[0042] Electrode cleaning: pass 0.5M H into the microchannel 2 SO 4 solution, and perform cyclic voltammetry scanning in the range of -0.2 to ...

Embodiment 2

[0048] The characteristic sequence was detected by self-assembled thiol DNA probe, and methylene blue (MB) was used as indicator.

[0049] In DNA electrochemical biosensors, single-stranded DNA molecules of known sequence (referred to as DNA probes) are immobilized on electrodes, and selectively recognize the target DNA (cDNA) sequence in the sample according to the principle of complementary base pairing. The hybridization process was monitored electrochemically. In this experiment, MB was used as an indicator, because MB has a special affinity for guanine in single-stranded DNA (ssDNA), and its interaction with guanine reduces the electron transporter, resulting in a decrease in the peak current; but when hybridization forms double When stranded DNA (dsDNA) is added, the free guanine on the electrode surface no longer binds to MB, and the peak current increases significantly. Therefore, the detection of characteristic DNA sequences can be achieved through this principle of ...

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Abstract

The invention relates to a method for designing and preparing an electrochemical detection-microfluidic multichannel chip based on a self-assembled monolayer technology. The method provided by the invention realizes designing and preparation of the electrochemical detection-microfluidic multichannel chip, utilizes the combination of the self-assembled monolayer technology and electrochemical detection in the field of the microfluidic, and realizes high-efficiency low-consumption detection of multiple substances. The method provided by the invention comprises the following steps of 1, designing and preparing electrode templates and microchannel templates corresponding to the electrode templates, 2, through microfluidic channels, fixing chemical molecules or biomolecules on the surfaces of microelectrodes through the self-assembled monolayer technology so that self-assembled layers are formed and modify the microelectrodes, 3, feeding an analyte through the microfluidic channels so that the analyte contacts with the modified microelectrodes and undergoes a reaction, and 4, feeding a test solution through the microfluidic channels and carrying out detection of the modified microelectrodes by an electrochemical method. The electrochemical detection-microfluidic multichannel chip obtained by the method has multiple electrode systems. The templates of the electrochemical detection-microfluidic multichannel chip can be prepared according to demands. The method provided by the invention can realize self-assembly and electrochemical detection of a single electrode or multi-electrodes.

Description

【Technical field】 [0001] The invention relates to the technical fields of microfluidic technology, electrochemical detection and self-assembly technology, and specifically relates to a design and preparation method of an electrochemical detection-microfluidic multi-channel chip based on self-assembly technology. 【Background technique】 [0002] Microfluidic technology, which appeared in the early 1980s, is an emerging field integrating engineering, physics, chemistry, biotechnology, and micro-manufacturing technology. Microfluidic technology can effectively and accurately control the behavior of fluids on a chip of a few centimeters square, so as to realize the measurement and analysis of some analytes. Among them, a series of complete experimental processes such as sample collection, pretreatment, separation, and detection on the chip are called "lab-on-a-chip". Microfluidic technology has the characteristics of controllable liquid flow, minimal consumption of samples and r...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N27/26
Inventor 蓝闽波陈晨赵红莉袁慧慧牛湘衡柴燕丁义丽
Owner EAST CHINA UNIV OF SCI & TECH
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