Rapid detection kit and detection method for v.parahaemolyticus multiple virulence factor GeXP

Abstract

The invention discloses a rapid detection kit and a detection method of a v.parahaemolyticus multiple virulence factor GeXP. The method comprises the following steps of: extracting a genome DNA (Deoxyribose Nucleic Acid) of the v.parahaemolyticus; performing multiple PCR (Polymerase Chain Reaction) amplification on 13 pairs of polygene PCR primers and a pair of general primers by taking the genome DNA as a template to obtain a multiple PCR reaction product; and detecting and analyzing by using a GenomeLab Gexp genetic analysis system. In the invention, a specific primer is designed specific to 13 kinds of known virulence genes of v.parahaemolyticus, and 13 kinds of genes are detected rapidly and efficiently at one time through a single-tube reaction to detect whether a sample contains v.parahaemolyticus which carries virulence genes and has potential pathogenic ability can be known, so that the detection coverage is wide, the accuracy of pathogenicity evaluation of v.parahaemolyticus can be greatly increased, and a more effective detection measure is provided for pathogenicity evaluation of v.parahaemolyticus. The method has important application prospects on the aspects of aquaculture disease detection, epidemiological investigation, pathogenic bacterium detection in foods, aquatic product import and export detection, hospital infection monitoring, and the like.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to a multiple virulence factor GeXP rapid detection kit and detection method for detecting pathogenic Vibrio parahaemolyticus (VP.) in clinical examination, public health and food inspection . Background technique: [0002] Vibrio parahaemolyticus (V.Parahaemolyticus) is a halophilic Gram-negative bacterium belonging to the genus Vibrio in the family Vibrio. It is a foodborne pathogenic bacterium mainly derived from seafood such as shrimp and shellfish. The bacteria can not only make seafood pathogenic, but also infect the human body, causing diarrhea, nausea, fever and other typical gastroenteritis reactions and food poisoning. [0003] According to the data released by the National Foodborne Disease Surveillance Network, the distribution of pathogens of microbial food poisoning in my country has changed significantly, especially in coastal provinces, the scale of food po...

AI Technical Summary

Problems solved by technology

However, in terms of pathogenicity detection of Vibrio parahaemolyticus, the defects of these methods are: the detection content is too single and cannot fully reflect the potential pathogenicity of the strain

Under natural circumstances, the vast majority of Vibrio parahaemolyticus belong to the normal flora in and on the surface of aquatic animals, and only a few strains with specific virulence genes are pathogenic. However, due to the diversity of virulence gene types , distribution differences, different pathogenic strains often carry different virulence genes

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