Nocardia culture medium

A Nocardia and culture medium technology, applied in the field of Nocardia culture medium, can solve the problems of misdiagnosis and achieve high separation rate and reliable detection

Inactive Publication Date: 2013-07-24
王京军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And if it is a sputum sample, it is likely to be covered by the normal flora in the sputum sample and cause a misdiagnosis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1, the formula for preparing 1000ml medium contains: cornstarch 2g; beef liver powder 20g; magnesium sulfate 0.8g; agar 15g; oxacillin 0.02g; , inositol 0.2g; add distilled water to 1000ml.

Embodiment 2

[0022] Example 2, the formula for preparing 1000ml medium contains: pea starch 4g; beef liver powder 10g; magnesium sulfate 1.1g; agar 20g; oxacillin 0.005g; , inositol 0.01g; add distilled water to 1000ml.

Embodiment 3

[0023] Example 3, the formula for preparing 1000ml medium contains: potato starch 2g; beef liver powder 20g; magnesium sulfate 0.8g; agar 15g; oxacillin 0.02g; , inositol 0.2g, thymidine 0.05g; add distilled water to 1000ml.

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PUM

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Abstract

The invention discloses a Nocardia culture medium. The Nocardia culture medium is characterized in that the formulation for preparing 1000 ml of the culture medium contains 2-4 g of soluble starch, 10-20 g of bovine liver powder, 0.005-0.02 g of oxacillin, 3-5 million units of multisubtilin, 0.1-0.4 g of allithiamine, 0.01-0.2 g of inositol, 0.8-1.1 g of magnesium sulfate, 15-20 g of agar, and distilled water which is added to reach the total volume of 1000 ml. Compared with the prior art, the Nocardia culture medium has the characteristics of reliable detection and high separation rate.

Description

technical field [0001] The invention relates to the field of test microorganism culture, in particular to a culture medium suitable for Nocardia. Background technique [0002] There are nearly 30 species of Nocardia genus, and nearly 10 species related to medicine. They belong to the genus Actinomycetes and are aerobic bacteria that exist in soil and can cause nocardiosis in humans. Bacteria-carrying dust, soil or food enters the human body through the respiratory tract, skin wound or digestive tract, and then is confined to a certain organ or tissue, or spreads to the brain, kidney or other organs through blood circulation. Because the bacterium is not very common and grows slowly, it takes several weeks to make a more accurate identification using traditional methods, so it is easy to misdiagnose clinically. Commonly used general antibiotics are used for treatment, resulting in delayed time, causing multiple lesions to metastasize, such as metastasizing to the brain, whic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04C12R1/04
Inventor 王京军李艳刘森
Owner 王京军
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