Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for constructing chroogomphis rutillus fruiting body surrounding soil fungus community internet testing services (ITS) library

A technology of rivet mushroom seeds and soil, which is applied in the field of constructing the ITS library of soil fungal communities around the fruiting body of blood red rivet mushroom, and achieves the effect of important application value

Active Publication Date: 2012-07-11
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on the microbial population in its growth environment has not been reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for constructing chroogomphis rutillus fruiting body surrounding soil fungus community internet testing services (ITS) library
  • Method for constructing chroogomphis rutillus fruiting body surrounding soil fungus community internet testing services (ITS) library
  • Method for constructing chroogomphis rutillus fruiting body surrounding soil fungus community internet testing services (ITS) library

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1. Construction of the ITS library of the soil fungal community around the fruiting body of the blood-red rivet mushroom

[0053] 1. Collection of samples

[0054] Soil samples were collected at an altitude of 790 meters in Beijing (GPS coordinates N4057.186 E11629.923) at a place 10 cm below the fruiting body of the rivet mushroom, and placed in a sterile kraft paper bag.

[0055] 2. Extraction of metagenomic DNA from soil samples

[0056] (1) Weigh 5g of the soil sample collected in step 1, suspend in 3ml TENP buffer, vortex and shake for 10min, centrifuge at 12000rpm for 10min, and take the precipitate.

[0057] (2) Wash the precipitate in step (1) three times with TENP buffer, and then add 3 ml of PBS buffer to wash once.

[0058] (3) The precipitation of step (2) is carried out the extraction of metagenomic group, the extraction of metagenomic group adopts Mega Preps PowerMax Soil DNA Isolation Kit (Mo Bio, Carlsbad, CA, USA) to carry out according to ins...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for constructing a chroogomphis rutillus fruiting body surrounding soil fungus community internet testing services (ITS) library. The method comprises the following steps of: extracting a metagenome of chroogomphis rutillus fruiting body surrounding soil; performing polymerase chain reaction (PCR) amplification by using the metagenome as a template and using ITS1 and ITS4, and reclaiming DNA fragments of 700bp; connecting each DNA fragment and a pMD19-T vector, performing PCR amplification by using RV-M and M13-47, performing enzyme digestion on each PCR product by using HinfI, Hae III, Msp I, Taq I and Mbo I respectively, and performing electrophoresis to obtain a molecular map. By using the method, the chroogomphis rutillus fruiting body surrounding soil fungus community ITS library can be constructed; and the method has significance for determining the growth environment microbial fungus community structure, the heredity and the functional diversity of chroogomphis rutillus fruiting bodies, provides important information for deeply discussing artificial acclimation and culture of chroogomphis rutillus, and has important application value.

Description

technical field [0001] The invention relates to a method for constructing an ITS library of a soil fungal community around the fruit body of the rivet mushroom. Background technique [0002] Blood red rivet mushroom (Chroogomphis rutillus), Basidiomycotina, Phytomycetes, Agaricaceae, Rivetaceae, Chroogomphis genus, commonly known as red mushroom, pine umbrella, pine mushroom, meat mushroom, shaped like a rivet, the color is dark Red, rare wild mycorrhizal edible fungus, is an important ectophytic large mycorrhizal fungus of coniferous trees, growing in groups or scattered in summer and autumn. The mushroom is thick, smooth, delicate, delicious, high in nutritional value, rich in protein, contains 8 kinds of essential amino acids, calcium, iron and other minerals and a large amount of carbohydrates needed by the human body, and is also a dietary fiber and natural antioxidant An important source of and loved by the people. [0003] Blood red rivet mushroom is an ectomycorrhi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10C12N15/63C12N15/11C12Q1/68C40B40/06C40B50/06A01G1/04
Inventor 许峰刘宇王守现赵爽尹永刚王鹏王兰青耿小丽孟莉莉
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com