New strains K05 and K03 of lentinus edodes and breeding method for strains

A new strain and strain technology, applied in the field of edible fungus breeding, can solve the problems of unstable transformants, over-density, and long-term use, and achieve obvious mutagenesis effects, improved production traits, and enhanced adaptability

Inactive Publication Date: 2012-07-11
YUANAN KELISON MUSHROOM
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Problems solved by technology

However, the main problems currently existing in genetic engineering breeding are: 1. The interference of false positive colonies; 2. The integration rate of exogenous DNA is not high; 3. The expression rate of exogenous genes is not high after integration; 4. The transformant is unstable
Lentinus edodes "135" strain is a low-temperature late-maturing variety used in the 1970s. It has good mushroom shape and quality, but it has been used for a long time. Rotten cylinder
Lentinus edodes "939" strain has a high yield and is easy to manage, but the mushrooms are too much, too dense, and there are many clusters of mushrooms, which seriously affect the quality of lentinus edodes

Method used

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  • New strains K05 and K03 of lentinus edodes and breeding method for strains
  • New strains K05 and K03 of lentinus edodes and breeding method for strains
  • New strains K05 and K03 of lentinus edodes and breeding method for strains

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Experimental program
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Embodiment Construction

[0028] 1. Materials and methods

[0029] 1.1 Materials

[0030] 1.1.1 Parental strains

[0031] Shiitake 135 (introduced by Sanming Institute of Fungi), Shiitake 303 (crossbreeding by our company).

[0032] 1.1.2 Medium

[0033] Test medium: PDA+20% fungus chaff boiled juice.

[0034] Mutation medium: PDA+0.1% colchicine.

[0035] Cultivation medium: miscellaneous sawdust 83%, bran 15%, sugar 1%, gypsum 1%.

[0036] 1.1.3 Main reagents

[0037] Colchicine (C22H25NO 5 ): 98% content, analytically pure, imported by Shanghai Huishi Biochemical Reagent Company.

[0038] Fluorescent dye: Hoechst33258 (trade name), bisbenzimidazole (C 25 h 24 N 5 o 13 HCL) chemical name, imported from Switzerland.

[0039] 1.2 Method

[0040] 1.2.1 Mutagenesis treatment of dikaryon strains

[0041] Dikaryon strains of Lentinus edodes 135 and 303 were respectively inoculated on slant medium containing 0.1% colchicine, cultured in a constant temperature incubator at 25°C, and each strain...

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Abstract

The invention relates to new strains K05 and K03 of lentinus edodes and a breeding method for the strains. The K05 is a dicaryon mutant strain of a lentinus edodes 135 strain, and the K03 is a dicaryon mutant strain of a lentinus edodes 303 strain. The strains are obtained by induced culture of a potato dextrose agar (PDA) culture medium containing colchicine; on the basis that the conventional excellent characters of the starting strains are not changed, compared with the staring strains, the production characters of the new strains on yield, quality, adaptability and the like are greatly promoted; and the method is a simple, convenient, practical and effective new edible fungus breeding technology.

Description

technical field [0001] The invention relates to new Lentinula edodes strains K05 and K03 and a breeding method thereof, belonging to the field of edible fungus breeding. Background technique [0002] At present, the breeding methods of edible fungi mainly include training of wild species, cross breeding, mutation breeding, protoplast fusion breeding and genetic engineering breeding. The training of wild species is to obtain mycelia from wild fruiting bodies, and obtain excellent strains through cultivation tests. This method needs to collect and isolate a large number of strains, from which to screen high-yield and high-quality varieties, but cannot create new species. Hybrid breeding is a widely used and effective breeding technology. It can combine the excellent traits of both parents, show strong hybrid vigor, and breed excellent hybrids that surpass the parents. However, hybrid breeding is a labor-intensive and time-consuming work. The process of determining the parent...

Claims

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Application Information

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IPC IPC(8): A01H1/08
Inventor 徐年声
Owner YUANAN KELISON MUSHROOM
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