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Aging oil biodesulfurization microbial agent and method for treatment of aging oil with microbial agent

A biological desulfurization and aging oil technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of electrode and hanging plate damage, loss of desulfurization effect, easy adhesion of sulfide, etc.

Inactive Publication Date: 2013-06-19
武汉华美天策生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] (1) A large amount of black water and black transition layer are generated in the whole system, which affects the control and adjustment of the automatic control instrument
[0006] (2) It has extremely strong conductivity. If the presence of sulfide in the electric dehydrator exceeds a certain ratio, the electric dehydrator will discharge and lose the point dehydration effect
And the sulfide is easy to adhere to the electrode insulating rod and insulating hanging plate of the electric dehydrator, causing damage and damage to the electrode and hanging plate
Oil-water interlayers are formed in the electric dehydrator, resulting in water in the exported crude oil or substandard sewage treatment
[0007] (3) When the upper part of the sewage settling tank is enriched with a ferrous sulfide transition layer, it is easy to cause the electric dehydrator to cross the electric field during crude oil recovery, which affects the normal oil recovery
This method fails to achieve the treatment effect of aging oil after only two tests, and the aging oil is still transported to the recycling station for centralized treatment

Method used

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  • Aging oil biodesulfurization microbial agent and method for treatment of aging oil with microbial agent
  • Aging oil biodesulfurization microbial agent and method for treatment of aging oil with microbial agent
  • Aging oil biodesulfurization microbial agent and method for treatment of aging oil with microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The water sample that will be taken from in the fish culture landscape pond is brought back to the laboratory and is used in aseptic room with 400mL enriched culture solution (in the purple sulfur bacteria seed culture medium, adding mass concentration is 2% Na 2 S·9H 2 O is the enrichment medium) into a 500mL Erlenmeyer flask, pour the collected water sample to the mouth of the bottle, airtight, oscillate and mix, and cultivate in a 37°C, 3000-5000lx light incubator for 10 days until cultured When the substance turns red, use a pipette gun to transfer 1mL of the enriched culture to another Erlenmeyer flask filled with fresh enriched culture solution. Repeat the above operation 2 to 3 times to obtain well-growing light and bacterial enrichment. Cultures. Then the enriched culture was diluted in a gradient, and then separated from the solid medium with agar added, and the colony was picked and cultured in the liquid medium (i.e. seed medium) for 7 days, and then repeate...

Embodiment 2

[0052] Soil samples collected from acidic (pH about 2 to 3) and moist soil (the sampling soil layer is 15cm away from the ground) were added to the Thiobacillus denitrifica seed medium in batches in a natural state, and the cultured soil samples were taken out after 3 weeks for separation nourish. Weigh 0.5g of the cultured soil sample, quickly pour it into a sterile water bottle with glass beads (the amount of glass beads should fill the bottom of the bottle is the best), shake for 5-10 minutes to fully break up the soil sample, which becomes 10 -2 soil suspension; use a sterile pipette to aspirate 10 -2 0.5mL of the soil suspension, put into 4.5mL sterile water and serve 10 -3 Dilution, so repeated, can make 10 -3 ~10 -8 After cultivation, take the turbid bacterial solution at the last level, dilute it in a gradient, and then separate it from the solid medium with agar added, pick but the colonies are cultured in the liquid medium for 7 days, and repeat the solid medium ...

Embodiment 3

[0055] The composition of the seed culture medium of purple sulfur bacteria PSE with the preservation number CCTCC NO: M2011276 is CH 3 COONa 3g / L, (NH 4 ) 2 SO 4 1.0g / L, MgSO 4 0.2g / L, NaCl 1g / L, KH 2 PO 4 0.3g / L, K 2 HPO 4 0.5g / L, CaCl 2 0.05g / L, yeast extract 0.1g, V B1 1Ml, 1L of distilled water, the isolated purple sulfur bacterium PSE with the preservation number CCTCC NO: M2011276 is cultured in the seed medium for 7 days under light, and the seed liquid of the bacteria can be obtained.

[0056] The composition of the seed culture medium of Thiobacillus denitrificans with the preservation number CCTCC NO: M2011135 is Na 2 HPO 4 1.2g / L, KH 2 PO 4 1.8g / L, NH 4 Cl 0.5g / L, KNO 3 5g / L, Na 2 S 2 o 3 10g / L, NaHCO 3 1g / L, MgSO 4 ·7H 2 O, CaCl 2 , MnSO 4 , FeCl 3 A small amount, 1L of distilled water, and the isolated Thiobacillus denitrificans with the preservation number CCTCC NO: M2011135 are placed in an oven at about 30°C for more than 7 da...

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Abstract

The invention belongs to the field of reuse of aging oil, and discloses an aging oil biodesulfurization microbial agent and a method for treatment of aging oil with the microbial agent. The aging oil biodesulfurization microbial agent mainly comprises purple sulfur bacteria PSE (Ectothiorhodospira sp.PSE) with the preservation number of CCTCC NO.: M2011276, thiobacillus denitrificans with the preservation number of CCTCC NO.: M2011135 and inorganic salts, and the quantities of the two types of bacteria are no less than 109 per mL. The method for treatment of the aging oil with the aging oil biodesulfurization microbial agent comprises culture of the aging oil biodesulfurization microbial agent in an aging oil biodesulfurization reactor, addition of the aging oil biodesulfurization microbial agent and desulfurization of the aging oil. The biodesulfurization microbial agent can be produced simply with low cost, the problems of environmental pollution and production caused by sulfides inthe aging oil can be solved, the efficiency of recovery and treatment of the aging oil can be improved, the cost can be reduced, and significant economic and social benefits can be achieved.

Description

technical field [0001] The invention belongs to the field of aging oil reuse, and relates to a biological desulfurization bacterial agent for aging oil and a method for treating aged oil with the biological desulfurization bacterial agent, in particular to a biological desulfurization bacterial agent for aging oil, and using the bacterial agent as a reaction medium to The sulfide in the aging oil is oxidized into sulfur element, and the aging oil is treated by using an aging oil biological treatment tank which can automatically control the removal of the sulfur compound in the aging oil and recover the sulfur element. Background technique [0002] With the continuous development of oil fields, the problem of aging oil treatment is becoming more and more serious. In the dehydration process of the combined station, the problem of difficult treatment of aged oil is often encountered. Overseas oilfields that are difficult to treat aged oil have all built aging oil treatment sys...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C10G32/00C12R1/01
Inventor 刘宏芳秦双
Owner 武汉华美天策生物科技有限公司
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