Anti-foot-and-mouth disease type O virus-like particle vaccine and preparation method thereof

Abstract

The invention discloses an anti-foot-and-mouth disease type O virus-like particle vaccine and a preparation method thereof, relating to the field of genetic engineering and immunology. The invention provides a safe and reliable anti-foot-and-mouth disease type O virus-like particle vaccine capable of effectively preventing the viruses of foot-and-mouth diseases, a preparation method of the particle vaccine, an amino acid sequence and a deoxyribonucleic acid (DNA) sequence of the anti-foot-and-mouth disease type O virus-like particle vaccine, a safe and stable prokaryotic expression vector 28a-CP-VP1 and the application of the anti-foot-and-mouth disease type O virus-like particle vaccine. The anti-foot-and-mouth disease type O virus-like particle vaccine has, shown in SEQ ID NO.1, the amino acid sequence which forms the anti-foot-and-mouth disease type O virus-like particle vaccine, and can be expressed by escherichia col and independently packed to be a virus-like particle structure in escherichia coli cells and exist as the virus-like particles. The vaccine can induce animal to generate anti-foot-and-mouth disease antibodies at the same time after immunization; the preparation is safe; and the vaccine has no pathogenic action probably caused by attenuated vaccine and inactivated vaccine.

Description

technical field [0001] The present invention relates to the fields of genetic engineering and immunology, in particular to the expression and preparation of an MS2-mediated virus-like particle vaccine against type O foot-and-mouth disease, the amino acid sequence of protein molecules constituting the virus-like particle and the corresponding gene sequence, and The preparation method and application of the virus particle. Background technique [0002] Foot-and-mouth disease is an acute, highly contagious, febrile infectious disease that harms artiodactyla animals, known for its rapid spread and high infectivity ([1] Bachrach, H.L., Moore, D.M., McKercher, P.D. & Polatnick, J.Immune and Antibody responses to an isolated capsid protein of foot-and-mouth disease virus. The Journal of Immunology 115, 1636 (1975); [2] Brooksby, J. Portraits of viruses: foot-and-mouth disease virus. Intervirology 18, 1-23 (1982); [3] Pereira, H.G. Foot-and-mouth disease.333-363 (1981); [4] Sobrino...

AI Technical Summary

Problems solved by technology

But there are also the following disadvantages: 1. The operation requirements are strict in the production process, and there are potential safety hazards

The safety of production is poor, and it is necessary to take protective measures for the laboratory and the staff participating in the experiment to ensure that they will not be contaminated by pathogenic substances and that the virus will not leak

At the same time, during the production process, the virus may not be completely killed or fully attenuated, which will cause the vaccine to contain highly toxic pathogenic substances, which will cause the disease of the immunized animals and cause a large-scale epidemic of the disease ([10]King, A., Underwood, B., McCahon, D., Newman, J. & Brown, F. Biochemical identification of viruses causing the 1981 outbreaks of foot and mouth disease in the UK. Nature293, 479-480(1981))

2. The validity period is very short, and often need to be frozen, which increases the difficulty of promoting the use of vaccines in rural areas

3. Inactivated vaccine immunity is indistinguishable from FMD-infected animals, which limits the export of animals

4. Production depends on FMDV, so FMDV cannot be completely eliminated

The study of displaying the epitope on VP1 of foot-and-mouth disease virus on the surface of virus-like particles of MS2 to form a foot-and-mouth disease vaccine has not been reported

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