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Method for using amantadine hydrochloride drug to culture lily under virus-free state

A technology for amantadine hydrochloride and lily, which is applied in the field of plant tissue culture rapid propagation technology and biology, can solve problems such as low survival rate of shoot tip culture, and achieve the effects of improving detoxification efficiency, high detoxification efficiency and short time.

Inactive Publication Date: 2012-05-09
杨凯 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The survival rate of shoot tip culture is low

Method used

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  • Method for using amantadine hydrochloride drug to culture lily under virus-free state
  • Method for using amantadine hydrochloride drug to culture lily under virus-free state
  • Method for using amantadine hydrochloride drug to culture lily under virus-free state

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0042] Embodiment: (the virus detection of virus-free tissue culture seedling)

[0043] (1) Plant material

[0044] The tissue culture seedlings of the control group and the experimental group were used as materials.

[0045] (2) Extraction of viral RNA

[0046] Adopt TRIzol reagent, carry out total RNA extraction to each test sample respectively, the preparation method of the RNase-free water used in the extraction process: fill up 0.1% (v / v) DEPC ultrapure water in the clean glass bottle, After overnight treatment, the glass bottle is autoclaved; RNase-free plastic and glassware treatment method: glassware can be baked at 150°C for 6 hours; plasticware is soaked in 0.5M NaOH for 10 minutes, then thoroughly washed with DEPC water, and then Autoclave. The specific operation steps are as follows:

[0047] 1) Put the test sample directly into a mortar, add a small amount of liquid nitrogen, quickly grind it into powder, and add 1ml TRIzol for every 50-100mg plant sample. Af...

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Abstract

The invention discloses a method for using an amantadine hydrochloride drug to culture lily under a virus-free state and belongs to the fields of plant tissue culturing fast propagation technique and biologic technique. The method comprises the following steps of: inspecting lily mother bulb virus, preparing a culture medium and reserving, sterilizing and inoculating explants, culturing the explants, inspecting virus of tissue culturing seedlings, and the like, thereby quickly obtaining lots of tissue culturing seedlings. According to an orthogonal method, a formula of the best induced culture medium of buds and root-less tissue culturing seedlings directly differentiated from lily is obtained; an amantadine hydrochloride antiviral drug with a series of concentration gradients is prepared and an optimum detoxifying concentration is selected, so that the detoxifying efficiency can reach 87%; a period of induced culturing is shortened, and meanwhile, the detoxifying efficiency is high and the enhancement factor is high; and a technical basis for researching on the culturing for lily virus-free plants and the popularization for virus-free culturing is provided.

Description

1. Technical field [0001] The invention discloses a method for detoxifying lilies by using amantadine hydrochloride agent, and belongs to the field of plant tissue culture rapid propagation technology and biotechnology. 2. Background technology [0002] Lily is the general name of the genus Lilium (Lilium) of the monocot class Liliaceae (Liliaceae), which is a perennial herbaceous flower. There are more than 90 species of Lilium in the world, and about 47 species originate in my country, accounting for 51% of the world total. There are many varieties of lilies, with huge flowers, rich colors, and beautiful flowers. They can be used as cut flowers, potted flowers, and in gardens and green spaces. Most lilies are edible, and they are the best nourishing products. Lily has the meaning of "a hundred years of harmony" and is deeply loved by people. Lilies are mainly distributed in temperate regions such as China, Japan, North America and Europe. In recent years, the production ...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12Q1/70C12Q1/68
Inventor 杨凯张克赵祥云安利清王树栋王文和
Owner 杨凯
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