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Efficient lily detoxification method

A lily mottled virus and lily technology, applied in the field of lily detoxification by inducing callus, lily detoxification, can solve the problems of difficult stem tip stripping, high technical difficulty, complicated process, etc., and achieve callus Good condition, high callus induction rate, and difficult operation

Inactive Publication Date: 2020-06-16
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The problems of this detoxification method are: the demand for detoxification materials is huge, the stem tip is not easy to strip and the survival rate is low, the process is complex, time-consuming, and the technical requirements are high, so it is difficult to apply to factory production
In addition, the Chinese patent No. CN201410586353.4 "Comprehensive detoxification method for lily bulbs", which first detoxifies by heat treatment at 36-38°C and humidity of 70-86% in a greenhouse environment, and then conducts external detoxification. Disinfection of implants and collection and induction of growth points. The problems of this detoxification method are: high equipment cost, difficulty in stripping growth points, and high technical difficulty. The formed detoxified bulb bulbs are seriously degraded. virus, but it is easy to be infected with the virus again, so the practical application value is low
In addition, the existing patents basically do not conduct virus detection on the lily bulbs before treatment to confirm whether the raw materials are infected with viruses, so the statistics of the virus-free rate are not scientific to a certain extent

Method used

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  • Efficient lily detoxification method
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  • Efficient lily detoxification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Embodiment 1 A kind of efficient lily detoxification method, the detailed steps of this lily detoxification method are as follows:

[0073] 1) Select healthy and non-rotten lily scales to extract RNA, and carry out virus detection by reverse transcription: select 0.5g of non-rotten and healthy lily scales, freeze them in liquid nitrogen, and use the Trizol method to extract RNA; then use takara reverse transcription reagent Carry out reverse transcription of cassette; Design the primers (see table 1) of cucumber mosaic virus, lily asymptomatic virus and lily mottled virus, adopt 20 microliter system to carry out PCR amplification, detect whether to contain these three kinds of viruses;

[0074] 2) Select healthy lily scales in the middle and inner layer for explant disinfection, and cultivate a large number of sterile seedlings with good growth: select bulbs with fewer diseased spots, peel off the healthy scales in the middle and inner layer, and wash them under running ...

Embodiment 2

[0078] Embodiment 2 A kind of efficient lily detoxification method, the detailed steps of this lily detoxification method are as follows:

[0079] 1) Select healthy and non-rotten lily scales to extract RNA, and carry out virus detection by reverse transcription: select 0.5g of non-rotten and healthy lily scales, freeze them in liquid nitrogen, and use the Trizol method to extract RNA; then use takara reverse transcription reagent Carry out reverse transcription of cassette; Design the primers (see table 1) of cucumber mosaic virus, lily asymptomatic virus and lily mottled virus, adopt 20 microliter system to carry out PCR amplification, detect whether to contain these three kinds of viruses;

[0080] 2) Select healthy lily scales in the middle and inner layer for explant disinfection, and cultivate a large number of sterile seedlings with good growth: select bulbs with fewer diseased spots, peel off the healthy scales in the middle and inner layer, and wash them under running ...

Embodiment 3

[0084]Embodiment 3 A kind of efficient lily detoxification method, the detailed steps of this lily detoxification method are as follows:

[0085] 1) Select healthy and non-rotten lily scales to extract RNA, and carry out virus detection by reverse transcription: select 0.5g of non-rotten and healthy lily scales, freeze them in liquid nitrogen, and use the Trizol method to extract RNA; then use takara reverse transcription reagent Carry out reverse transcription of cassette; Design the primers (see table 1) of cucumber mosaic virus, lily asymptomatic virus and lily mottled virus, adopt 20 microliter system to carry out PCR amplification, detect whether to contain these three kinds of viruses;

[0086] 2) Select healthy lily scales in the middle and inner layer for explant disinfection, and cultivate a large number of sterile seedlings with good growth: select bulbs with fewer diseased spots, peel off the healthy scales in the middle and inner layer, and wash them under running w...

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Abstract

The invention discloses an efficient lily detoxification method which comprises the following steps: 1) selecting healthy and non-rotten lily scales to extract RNA, and carrying out virus detection through reverse transcription; 2) selecting lily scales with healthy middle and inner layers for explant disinfection, and culturing to obtain a large number of aseptic seedlings with good growth vigor;3) cutting off small scales of the aseptic seedlings, and inducing to obtain compact calluses; 4) performing differentiation culture on the calluses and inducing the calluses into seedlings; and 5) cutting the leaves in the step 4) for virus detection, wherein the lily virus-free seedlings are qualified. According to the lily detoxification method, the non-toxic seedlings are rapidly obtained byinducing the calluses, the problems that an existing lily detoxification process is complex, high in technical requirement and long in consumed time are solved, the lily detoxification method has theadvantages of being high in detoxification efficiency, simple in process and low in production equipment requirement, and the industrial production requirement can be met.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture and detoxification, and relates to a lily detoxification method, in particular to a lily detoxification method by inducing callus for detoxification. Background technique [0002] As a bulbous flower, lily occupies a very important position in the flower family. But its long-term use of bulbs, scales, bulbils and other vegetative bodies for asexual reproduction is very prone to virus infection, and often accompanied by bulbous degeneration, which seriously affects the quality and yield of lily. Among them, viral diseases are one of the main diseases that have occurred worldwide in recent years. With the rapid increase of the import, introduction and planting area of ​​lily in my country, as well as the irregular planting and self-propagation of bulbs, viral diseases began to spread in the lily planting areas of my country. The general incidence rate is 40%-50%, and the disease-carry...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 滕年军吴慧君吴泽张德花蓝令
Owner NANJING AGRICULTURAL UNIVERSITY
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