Preparation method of keratin material for biomedical engineering

Abstract

The invention relates to preparation of a keratin material used in the field of biomedical engineering. The keratin material is prepared by washing human hair and nails used as raw materials with water, drying, bleaching, washing with water, drying, adding 1-15% by weight of imidazolium chloride ionic liquid, and stirring and dissolving at 100-150 DEG C for 2-10 hours to obtain colloidal keratin ionic liquid; removing ionic liquid from the colloidal keratin ionic liquid with a water dialysis method to obtain a cross-linked keratin hydrogel solution; or spraying methanol or acetonitrile to the colloidal keratin ionic liquid to separate out keratin, drying and grinding into powder; pressing keratin into a film shape, a granular shape and various irregular structures and drying; and after the above processes, detecting residual amount of imidazolium chloride. The obtained keratin material is suitable for injury repairing materials, skin coating materials, medical beauty materials and tissue adhesion prevention materials which are used in biomedical engineering.

Description

Field of invention: [0001] The present invention is the preparation of keratin material used in the field of biomedical engineering. It is used in the manufacture of various implant materials, wound covering materials, tissue engineering frame materials, repairing nerve defects, repairing tendon, ligament defects, and soft tissue filling materials. Background technique: [0002] Human hair is the only human-derived protein that can be obtained in large quantities in vitro. Its physical properties have strong mechanical properties: namely, toughness, elasticity and stretch resistance. potential ability. Furthermore, since it is easy to obtain materials, there is no damage to the donor, and materials can be obtained repeatedly. As early as the 1960s, hair as a biological material attracted the attention of scholars (United States Patent 2993794 Process for preparing keratin protein). [0003] Structure and Composition of Human Hair Hair is derived from keratinized epitheliu...

AI Technical Summary

Problems solved by technology

With the in-depth research on keratin biomaterials and the improvement of its performance requirements, the problems of the traditional production process of keratin have been exposed: firstly, the reagent group of strong acid, strong alkali, strong reduction and strong oxidation method has poor targeting , in addition to destroying the disulfide bond and hydrogen bond of the protein, the peptide bond of the primary structure of the protein is severely damaged, and the molecular weight of the regenerated protein is low, and the molecular weight of the regenerated keratin is proportional to its material strength

Second, the traditional technology uses a large amount of environmentally unfriendly reagents such as inorganic acids and reducing agents, the production process is complicated, and there are many problems in the environmental follow-up treatment