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Biomass enzymolysis and saccharification pretreatment method

A biomass and pretreatment technology, applied in fermentation and other directions, can solve problems such as low efficiency of direct enzymatic hydrolysis and saccharification, and achieve the effects of favorable promotion, mild conditions and easy control.

Inactive Publication Date: 2012-04-04
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the low efficiency of direct enzymatic hydrolysis and saccharification of natural lignocellulosic raw materials, the present invention provides a pretreatment method of biomass enzymolysis and saccharification. Firstly, the lignocellulosic biomass is soaked in a weak alkaline solution and then subjected to cooking pretreatment, and then ground Pulp, pulp after grinding and enzymatic hydrolysis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Accurately measure 697ml of water into a beaker, add 2.5gNaOH and 20gNaOH in sequence 2 SO 3 , Stir constantly to make it fully dissolved, and then a weakly alkaline solution can be obtained. Add the above-mentioned weak alkaline solution into a bucket containing 250g of dry eucalyptus wood chips, stir well to make the chips all wet, and soak for 12 hours at room temperature;

[0022] (2) After the pre-impregnation reaches the specified time, transfer the above wood chips together with the impregnation solution to an electric heating rotary cooking pot for cooking pretreatment, set the cooking temperature to 180°C, the heating time to 60 minutes, and the holding time to 120 minutes. wood chips for further processing;

[0023] (3) The wood chips obtained after cooking are subjected to two-stage refining with an atmospheric pressure high-consistency refiner, wherein the gap between the first stage and the second stage is 0.1mm. After grinding, the slurry is washed,...

Embodiment 2

[0027] (1) Accurately measure 697ml of water into a beaker, add 5.0gNaOH and 25gNaOH in sequence 2 SO 3 , Stir constantly to make it fully dissolved, and then a weakly alkaline solution can be obtained. Add the above-mentioned weak alkaline solution into a bucket containing 250g of dry eucalyptus wood, stir fully to make the wood chips all wet, and soak for 10 hours at room temperature;

[0028] (2) After the pre-impregnation reaches the specified time, transfer the above-mentioned wood chips together with the impregnation solution to an electric heating rotary cooking pot, set the cooking temperature to 170°C, the heating time to 45 minutes, and the holding time to 180 minutes for cooking pretreatment. After the pretreatment, the sample is collected for the next step of processing;

[0029] (3) The wood chips obtained after cooking are subjected to two-stage refining through an atmospheric pressure high-consistency refiner, in which the gap between the first and second stag...

Embodiment 3

[0033] (1) Accurately measure 697ml of water into a beaker, add 7.5gNaOH and 30gNaOH in sequence 2 SO 3 , Stir constantly to make it fully dissolved, and then a weakly alkaline solution can be obtained. Add the above-mentioned weak alkaline solution into a bucket containing 250g of dry eucalyptus wood, stir well to make the wood chips all wet, and soak for 8 hours at room temperature.

[0034] (2) After the pre-impregnation reaches the specified time, transfer the above-mentioned wood chips together with the impregnation solution to an electric heating rotary cooking pot, set the cooking temperature to 160°C, the heating time to 30 minutes, and the holding time to 240 minutes for cooking pretreatment. After the pretreatment, the sample is collected for the next step of processing;

[0035] (3) The wood chips obtained after cooking are subjected to two-stage refining through an atmospheric pressure high-consistency refiner, wherein the gap between the first stage and the seco...

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Abstract

The invention relates to a biomass enzymolysis and saccharification pretreatment method, which comprises the following steps of: mixing an aired wood fiber biomass raw material and alkalescent solution, preimpregnating, decatizing at the temperature of between 160 and 190 DEG C, keeping the temperature for 120 to 240 minutes, and heating for 30 to 60 minutes; performing two-section defibrination on the decatized slurry, wherein in a first section, a gap of an abrasive disc is between 0.3 and 0.4 millimeter, and in a second section, a gap of the abrasive disc is between 0.1 and 0.15 millimeter; and adding cellulose into slurry subjected to the defibrination, and performing enzymolysis to obtain enzymolysis saccharification liquid. In the biomass enzymolysis and saccharification pretreatment method, the biomass raw material is pretreated by a pretreatment method of combining thermochemistry and machinery, and is subjected to enzymolysis and saccharification; and by analyzing ingredients of the enzymolysis liquid, the yield of saccharide in the enzymolysis liquid is high, and subsequent fermentation inhibitors such as furfuraldehyde, acetic acid and the like are avoided.

Description

technical field [0001] The invention relates to biomass pretreatment, in particular to a biomass enzymatic saccharification pretreatment method. Background technique [0002] Woody biomass resources are organic resources produced by plants through photosynthesis. They are mainly composed of three polymer substances, cellulose, hemicellulose and lignin, such as trees and crop straws. They are the most abundant carbohydrate resources in the world. With the increasing depletion of non-renewable resources on the earth, it is of great significance to use biotechnology to bioconvert woody biomass. Enzymatic hydrolysis can convert the cellulose and hemicellulose into high value-added products such as monosaccharides, oligosaccharides, and organic acids, and improve the utilization rate. This is a research hotspot in the field of biomass science at present. However, since the surface of natural fibers has resistance to biological enzymes, the efficiency of direct enzymatic treatmen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14
Inventor 武书彬尉慰奇
Owner SOUTH CHINA UNIV OF TECH
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